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Törjék O, Witucka-Wall H, Meyer RC, Korff M, Kusterer B, Rautengarten C et al.. Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci. Theor Appl Genet 113: 1551-1561

Institute of Plant Breeding, Seed Science and Population Genetics, Hohenheim University, Stuttgart, Baden-Württemberg, Germany
Theoretical and Applied Genetics (Impact Factor: 3.79). 12/2006; 113(8):1551-61. DOI: 10.1007/s00122-006-0402-3
Source: PubMed

ABSTRACT

A new large set of reciprocal recombinant inbred lines (RILs) was created between the Arabidopsis accessions Col-0 and C24 for quantitative trait mapping approaches, consisting of 209 Col-0 x C24 and 214 C24 x Col-0 F(7 )RI lines. Genotyping was performed using 110 evenly distributed framework single nucleotide polymorphism markers, yielding a genetic map of 425.70 cM, with an average interval of 3.87 cM. Segregation distortion (SD) was observed in several genomic regions during the construction of the genetic map. Linkage disequilibrium analysis revealed an association between a distorted region at the bottom of chromosome V and a non-distorted region on chromosome IV. A detailed analysis of the RILs for these two regions showed that an SD occurred when homozygous Col-0 alleles on chromosome IV coincided with homozygous C24 alleles at the bottom of chromosome V. Using nearly isogenic lines segregating for the distorted region we confirmed that this genotypic composition leads to reduced fertility and fitness.

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Available from: Carsten Rautengarten, Jun 05, 2015
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    • "The relative length of the chromosomes in cM is larger than has been seen in maps with other populations (Lister and Dean, 1993;Alonso-Blanco et al., 1999;Loudet et al., 2002;Clerkx et al., 2004). We note an unusually large amount of segregation distortion in our population, especially on chromosome 5. Chromosome 5 distortions have also been seen to a lesser extent in other populations (Loudet et al., 2002;el-Lithy et al., 2006;Törjék et al., 2006). The basis for segregation distortion is unclear, partly because it could be the result of a number of different phenomena, including postzygotic selection, pollenpistil interactions, gamete competition, epistatic interactions , and/or seed abortion (Hormaza and Herrero, 1992;Li et al., 1997;Lord and Russell, 2002;Lu et al., 2002). "
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