Törjék O, Witucka-Wall H, Meyer RC, Korff M, Kusterer B, Rautengarten C et al.. Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci. Theor Appl Genet 113: 1551-1561

Institute of Plant Breeding, Seed Science and Population Genetics, Hohenheim University, Stuttgart, Baden-Württemberg, Germany
Theoretical and Applied Genetics (Impact Factor: 3.79). 12/2006; 113(8):1551-61. DOI: 10.1007/s00122-006-0402-3
Source: PubMed


A new large set of reciprocal recombinant inbred lines (RILs) was created between the Arabidopsis accessions Col-0 and C24 for quantitative trait mapping approaches, consisting of 209 Col-0 x C24 and 214 C24 x Col-0 F(7 )RI lines. Genotyping was performed using 110 evenly distributed framework single nucleotide polymorphism markers, yielding a genetic map of 425.70 cM, with an average interval of 3.87 cM. Segregation distortion (SD) was observed in several genomic regions during the construction of the genetic map. Linkage disequilibrium analysis revealed an association between a distorted region at the bottom of chromosome V and a non-distorted region on chromosome IV. A detailed analysis of the RILs for these two regions showed that an SD occurred when homozygous Col-0 alleles on chromosome IV coincided with homozygous C24 alleles at the bottom of chromosome V. Using nearly isogenic lines segregating for the distorted region we confirmed that this genotypic composition leads to reduced fertility and fitness.

Download full-text


Available from: Carsten Rautengarten, Jun 05, 2015
  • Source
    • "The relative length of the chromosomes in cM is larger than has been seen in maps with other populations (Lister and Dean, 1993;Alonso-Blanco et al., 1999;Loudet et al., 2002;Clerkx et al., 2004). We note an unusually large amount of segregation distortion in our population, especially on chromosome 5. Chromosome 5 distortions have also been seen to a lesser extent in other populations (Loudet et al., 2002;el-Lithy et al., 2006;Törjék et al., 2006). The basis for segregation distortion is unclear, partly because it could be the result of a number of different phenomena, including postzygotic selection, pollenpistil interactions, gamete competition, epistatic interactions , and/or seed abortion (Hormaza and Herrero, 1992;Li et al., 1997;Lord and Russell, 2002;Lu et al., 2002). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. In order to map the loci that control female-mediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) population derived from a cross between Arabidopsis thaliana accessions Vancouver (Van-0) and Columbia (Col-0) and mapped quantitative trait loci (QTL) responsible for nonrandom mating and seed yield traits. We genotyped a population of 490 recombinant inbred lines. A subset of these lines was used to construct an expanded map of 1061.4 cM with an average interval of 6.7±5.3 cM between markers. QTL were then mapped for female- and male-mediated nonrandom mating and seed yield traits. To map the genetic loci responsible for female-mediated nonrandom mating and seed yield, we performed mixed pollinations with Col-NPTII and Van-0 pollen on RIL pistils. To map the loci responsible for male-mediated nonrandom mating and seed yield, we performed mixed pollinations with Col-NPTII and RIL pollen on Van-0 pistils. Composite interval mapping of these data identified four QTLs that control female-mediated nonrandom mating, and five QTLs that control female-mediated seed yield. We also identified four QTLs that control male-mediated nonrandom mating, and three QTLs that control male-mediated seed yield. Epistasis analysis indicates that several of these loci interact. To our knowledge, the results of these experiments represent the first time female-mediated nonrandom mating has been genetically defined.
    Full-text · Article · Mar 2014 · Plant physiology
  • Source
    • "Segregation distortion is a common fact in segregated populations generated from crosses between diverse genotypes [64,65]. In plants, segregation distortion was first reported in maize [66], and subsequently in many species including rice [67], wheat [68], Arabidopsis [69], and cabbage [14]. Segregation distortion can have important implications for the construction of a genetic map and QTL mapping, but if addressed properly, distorted markers can also be helpful for QTL mapping [70]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Expressed sequence tag (EST)-based markers are preferred because they reflect transcribed portions of the genome. We report the development of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers derived from transcriptome sequences in cabbage, and their utility for map construction. Transcriptome sequences were obtained from two cabbage parental lines, C1184 and C1234, which are susceptible and resistant to black rot disease, respectively, using the 454 platform. A total of 92,255 and 127,522 reads were generated and clustered into 34,688 and 40,947 unigenes, respectively. We identified 2,405 SSR motifs from the unigenes of the black rot-resistant parent C1234. Trinucleotide motifs were the most abundant (66.15%) among the repeat motifs. In addition, 1,167 SNPs were detected between the two parental lines. A total of 937 EST-based SSR and 97 SNP-based dCAPS markers were designed and used for detection of polymorphism between parents. Using an F2 population, we built a genetic map comprising 265 loci, and consisting of 98 EST-based SSRs, 21 SNP-based dCAPS, 55 IBP markers derived from B. rapa genome sequence and 91 public SSRs, distributed on nine linkage groups spanning a total of 1,331.88 cM with an average distance of 5.03 cM between adjacent loci. The parental lines used in this study are elite breeding lines with little genetic diversity; therefore, the markers that mapped in our genetic map will have broad spectrum utility. This genetic map provides additional genetic information to the existing B. oleracea map. Moreover, the new set of EST-based SSR and dCAPS markers developed herein is a valuable resource for genetic studies and will facilitate cabbage breeding. Additionally, this study demonstrates the usefulness of NGS transcriptomes for the development of genetic maps even with little genetic diversity in the mapping population.
    Full-text · Article · Feb 2014 · BMC Genomics
  • Source
    • "and do not indicate substantial two - locus linkage disequilibrium as has been previously described in the progeny from some A . thaliana crosses ( Törjék et al . , 2006 ; Simon et al . , 2008 ) . QTL detection was performed using the R / qtl package ( Broman et al . , 2003"
    [Show abstract] [Hide abstract]
    ABSTRACT: Whole-genome duplication resulting from polyploidy is ubiquitous in the evolutionary history of plant species. Yet, polyploids must overcome the meiotic challenge of pairing, recombining, and segregating more than two sets of chromosomes. Using genomic sequencing of synthetic and natural allopolyploids of Arabidopsis thaliana and Arabidopsis arenosa, we determined that dosage variation and chromosomal translocations consistent with homoeologous pairing were more frequent in the synthetic allopolyploids. To test the role of structural chromosomal differentiation versus genetic regulation of meiotic pairing, we performed sequenced-based, high-density genetic mapping in F2 hybrids between synthetic and natural lines. This F2 population displayed frequent dosage variation and deleterious homoeologous recombination. The genetic map derived from this population provided no indication of structural evolution of the genome of the natural allopolyploid Arabidopsis suecica, compared with its predicted parents. The F2 population displayed variation in meiotic regularity and pollen viability that correlated with a single quantitative trait locus, which we named BOY NAMED SUE, and whose beneficial allele was contributed by A. suecica. This demonstrates that an additive, gain-of-function allele contributes to meiotic stability and fertility in a recently established allopolyploid and provides an Arabidopsis system to decipher evolutionary and molecular mechanisms of meiotic regularity in polyploids.
    Full-text · Article · Jan 2014 · The Plant Cell
Show more