A rapid HPLC method for simultaneous determination of tretinoin and isotretinoin in dermatological formulations
A rapid method using an isocratic high-pressure liquid chromatography and UV detection for determination of both all-trans retinoic acid (tretinoin) and 13-cis retinoic acid (isotretinoin) in dermatological preparations is presented. Tretinoin and isotretinoin samples were extracted with acetonitrile by a procedure that can be completed in less than 10 min. Subsequent separation and quantification of amounts as low as 10 pmol was accomplished in less than 15 min using reversed-phase HPLC with isocratic elution with 0.01% trifluoroacetic acid (TFA)/acetonitrile (15:85, v/v). Validation experiments confirmed the precision and accuracy of the method. When applied to commercial tretinoin samples, recoveries of 104.9% for cream formulations and 107.7% for gel formulations were obtained. Application of the method for analysis of a tretinoin cream exposed to solar simulated light (SSL) demonstrated detection of the major photoisomerization product isotretinoin as well as 9-cis retinoic acid, demonstrating the utility of the method for studies of tretinoin photostability. The method should also facilitate studies of the formulation compatibility and photocompatibility of tretinoin with agents that may improve its clinical tolerability.
Available from: Maliheh Barazandeh
- "There are some reported articles in the literature for the determination of clindamycin [3-8] or tretinoin [9,10] alone or via the combination of the two drugs  by HPLC and gas chromatography in bulk or in pharmaceutical dosage form. HPLC method has been used in the USP35- NF30 for determination of clindamycin phosphate and tretinoin in separate gel dosage form but no simultaneous method is reported . "
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ABSTRACT: A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at (1D) 251 nm and (2D) 239 nm and tretinoin at (1D) 364 nm and (2D) 387 nm.
The proposed method showed excellent linearity at both first and second derivative order in the range of 60–1200 and 1.25–25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CV<3.81%, error<3.20%). Good agreement between the found and added concentrations indicates successful application of the proposed method for simultaneous determination of clindamycin and tretinoin in synthetic mixtures and pharmaceutical dosage form.
Available from: Farid Menaa
- "HPLC , Microcalorimetry , UV radiation monitor , thin layer chromatography (TLC) and preparative layer chromatography (PLC) , capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (ME-KC)  have been also employed to study retinoid degradation products in commercial preparation and raw materials. Although the above methods are able to separate and quantify isotretinoin, the extraction procedure is quite complex and HPLC analysis involves long retention times . In that context, the aim of this study was to develop a rapid method using an isocratic high performance liquid chromatography (HPLC) coupled to UV-vis detector to determine and quantify the isotretinoin in soft capsule formulations from three commercial samples. "
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ABSTRACT: The development of facile and rapid quantification of biologically active biomolecules such as isotretitoin in therapeutic drugs contained in many generic formu- lations is necessary for determining their efficiency and their quality to improve the human health care. Isotretritoin finds its applications in the maintenance of epithelial tissues. Different processes to date such as normal phase HPLC, or gas chromatrography am- ong others are able to separate and quantify isote- troin. However, the extraction is quite complex and in the case of HPLC, the analysis requires long retention times. In such context, an isocratic reversed- phase high-performance liquid chromatography (HP- LC) technique coupled with an UV-vis detector is described here for easy separation and quantification of 13-cis-retinoic (isotretinoin) from soft gelatin capsule formulations. The isotretinoin was extracted from three different commercial drug samples with tetrahydrofuran (THF) solvent by a procedure that can be completed in less than 10 minutes. Subsequent separation and quantification were accomplished in less than 5 minutes under isocratic reversed-phase conditions on a Lichrospher RP18 column and a mobile phase consisting of 0.01% TFA/acetonitrile (15/85, v/v) at a flow rate of 1.0 mL/min. Isotretoin was detected for the three samples via its UV-vis absorbance at 342 nm. The method was validated and the results showed good linearity, precision and accuracy for sensitive and selective quantitative determination of isotretinoin in the different pharmaceutical formulations. We found that the average isotretinoin content in two of the three commercial pro- ducts fell outside the 90-110% United States Pha- rmacopeia specifications. Consequently, the facile extraction and the precise method for the biomole- cule quantification open up tremendous possibilities in improving the quality control of drugs which can exist as different generic brands.
Available from: Kakasaheb R Mahadik
- "It acts by binding to the 50s subunit of the bacterial 70s rRNA complex, protein synthesis and subsequently structure/function processes critical for life or replication are inhibited  . Literature review reveals that several analytical methods have been reported for Isotretinoin    and Erythromycin       as individual determination or in biological fluids or in combination with other drugs in pharmaceutical dosage forms. A comprehensive literature survey revealed that no method has been reported for simultaneous estimation of Isotretinoin and Erythromycin by HPTLC in pharmaceutical dosage forms. "
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ABSTRACT: A simple, precise and accurate high performance thin layer chromatographic method has been developed for the simultaneous estimation of Isotretinoin and Erythromycin in pharmaceutical gel. The separation was car-ried out on Merck TLC aluminum sheets of silica gel 60 F 254 , (20 × 10 cm) with 250 µm thickness using toluene: DMSO: methanol (6.5:0.2:2.5, v/v/v) as a mobile phase. HPTLC separation of the two drugs fol-lowed by densitometric measurement of their spots at 340 nm for Isotretinoin before derivatization and 410 nm for Erythromycin after derivatization with 10% H 2 SO 4 and heating at 100C for 15 min. The drugs were satisfactorily resolved with R F values of 0.38 ± 0.02 and 0.55 ± 0.02 for Isotretinoin and Erythromycin, re-spectively. The accuracy and reliability of the method was assessed by evaluation of linearity (30-150 ng spot -1 for Isotretinoin and 1200-6000 ng spot -1 for Erythromycin), precision (intra-day RSD 0.62-0.79% and inter-day RSD 0.43-0.71% for Isotretinoin and intra-day RSD 0.47-1.71% and inter-day RSD 0.42-1.49% for Erythromycin), accuracy (98.91 ± 0.92% for Isotretinoin and 99.27 ± 0.72% for Erythromycin), and specific-ity, in accordance with ICH guidelines.
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