Article

Eukaryotic genome size database

Department of Integrative Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Nucleic Acids Research (Impact Factor: 9.11). 02/2007; 35(Database issue):D332-8. DOI: 10.1093/nar/gkl828
Source: PubMed

ABSTRACT

Three independent databases of eukaryotic genome size information have been launched or re-released in updated form since
2005: the Plant DNA C-values Database (www.kew.org/genomesize/homepage.html), the Animal Genome Size Database (www.genomesize.com) and the Fungal Genome Size Database (www.zbi.ee/fungal-genomesize/). In total, these databases provide freely accessible genome size data for >10 000 species of eukaryotes assembled from more
than 50 years' worth of literature. Such data are of significant importance to the genomics and broader scientific community
as fundamental features of genome structure, for genomics-based comparative biodiversity studies, and as direct estimators
of the cost of complete sequencing programs.

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    • "Despite the advent of NGS, developing fungal microsatellite markers is still challenging (Dutech et al. 2007; Schoebel et al. 2013a, b). While the small size of fungal genomes, most ranging from 10 to 60 Mbp (Gregory et al. 2007), makes it easier to explore the genome to find microsatellite loci, it appears that microsatellites are less abundant in fungi than in any other organism (Dutech et al. 2007). Fungal microsatellite motifs are also known to have low numbers of repetitions (most fewer than eight), with a high proportion of mononucleotide motifs, making it difficult to find highly polymorphic SSRs (Dutech et al. 2007). "
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    • "Glomeromycota genome size estimations showed great variations according to species, ranging from 0.18 to 1.08 pg of DNA per nucleus (Bianciotto & Bonfante, 1992; Hosny, Gianinazzi-Pearson, & Dulieu, 1998), that is, 176 Mb to over 1 Gb using the conversion formula of Doležel, Bartoš, Voglmayr, and Greilhuber (2003). Glomeromycota hence present the largest genome sizes among fungi (37 Mb on average—Gregory et al., 2007). Flow cytometry assays performed on isolate DAOM197198 first led to a genome size estimation of around 15 Mb (Hijri & Sanders, 2004), but later measurements using different standards indicated that the genome size could be in fact 10 times higher (154.8 "
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