Article

Inhibitory effects of neoandrographolide on nitric oxide and prostaglandin E2 production in LPS-stimulated murine macrophage

Institute of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Zhangjiang Hi-Tech Park, Shanghai, 201203, P. R. China.
Molecular and Cellular Biochemistry (Impact Factor: 2.39). 04/2007; 298(1-2):49-57. DOI: 10.1007/s11010-006-9349-6
Source: PubMed

ABSTRACT

Activated macrophages express inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), produce excessive amounts of nitric oxide (NO) and prostaglandin E(2) (PGE(2)), which play key roles in the processes of inflammation. Andrographis paniculata Nees is a traditional Chinese herb commonly used for treatment of infection, inflammation, and diarrhea. However, the mechanism of its therapeutic function is not well known. In the present study, the effect of neoandrographolide, one of bioactive components in A. paniculata, on iNOS-mediated NO production and COX-2-mediated PGE(2) in bacterial lipopolysaccharide (LPS) stimulated-murine macrophages was investigated. Neoandrographolide at concentrations (30-90 microM) significantly (p<0.05) inhibited the productions of NO and PGE(2) in LPS stimulated macrophages without inducing cytotoxicity. The effect of neoandrographolide also has been investigated on iNOS and COX-2 expression in activated macrophage by using RT-PCR and immunoblotting. The inhibition of NO release by neoandrographolide can be attributed to the block of iNOS mRNA transcription followed by inhibiting protein expression. However, neoandrographolide inhibited COX-2 protein expression only but without inhibiting COX-2 mRNA expression, which was involved in the inhibitory activity against the PGE(2 )overproduction. This suggests that the effect of neoandrographolide on iNOS expression may occur at the transcriptional level and the inhibition of COX-2 expression occurs at the translational level. Furthermore, we have found that the addition of neoandrographolide inhibited the activation of p38 mitogen-activated protein kinase (MAPKs) instead of JNK, ERK1/2, or NF-kappaB. These results indicated that the anti-inflammatory properties of neoandrographolide might result from the inhibition of iNOS and COX-2 expression through inhibiting p38 MAPKs activation. Therefore, neoandrographolide isolated from A. paniculata could be offered as a leading compound for anti-inflammation.

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Available from: Lili Ji, Nov 05, 2014
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    • "Antiviral (against chikungunya) Wintachai et al. (2015) Anti-hepatitis C virus activity Lee et al. (2014) Antihyperglycaemic Rammohan (2009) Neoandrographolide Antipyretic activity Deng (1978); Deng et al. (1982) Anti-inflammatory and antioxidant activities Batkhuu et al. (2002); Liu et al. (2007) Hepatoprotective Mohamed Saleem (2010) 14-deoxy-11,12- didehydroandrographolide Antipyretic Deng et al. (1982) Anti-inflammatory and anti-oxidant activities Parichatikanond et al. (2010); Yin and Guo (1993); Kumar et al. (2004); Deng et al. (1982) Anticancer activity Geethangili et al. (2008); Kumar et al. (2004) Cardiovascular activity Sheeja et al. (2007); Woo et al. (2008); Thisoda et al. (2006); Reyes et al. (2006) 14-deoxyandrographolide Antipyretic Deng (1978); Deng et al. (1982) Anti-inflammatory effect Anticancer activity Kumar et al. (2004) Andrographiside Hepatoprotective effect Kapil et al. (1993) The chemical constituents present in A. serpyllifolia exert biological activity of the flavones like apigenin 7, 4 " -dimethyl ether, tectochrysin, acylated flavone and glucosides components with their specific activity. Serpyllin, which comes under the category of flavone, has been isolated from A. serpyllifolia and its structure has been determined as 5-hydroxy-7, 8, 2′, 3′, 4′- pentamethoxyflavone. "

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    • "Antiviral (against chikungunya) Wintachai et al. (2015) Anti-hepatitis C virus activity Lee et al. (2014) Antihyperglycaemic Rammohan (2009) Neoandrographolide Antipyretic activity Deng (1978); Deng et al. (1982) Anti-inflammatory and antioxidant activities Batkhuu et al. (2002); Liu et al. (2007) Hepatoprotective Mohamed Saleem (2010) 14-deoxy-11,12- didehydroandrographolide Antipyretic Deng et al. (1982) Anti-inflammatory and anti-oxidant activities Parichatikanond et al. (2010); Yin and Guo (1993); Kumar et al. (2004); Deng et al. (1982) Anticancer activity Geethangili et al. (2008); Kumar et al. (2004) Cardiovascular activity Sheeja et al. (2007); Woo et al. (2008); Thisoda et al. (2006); Reyes et al. (2006) 14-deoxyandrographolide Antipyretic Deng (1978); Deng et al. (1982) Anti-inflammatory effect Anticancer activity Kumar et al. (2004) Andrographiside Hepatoprotective effect Kapil et al. (1993) The chemical constituents present in A. serpyllifolia exert biological activity of the flavones like apigenin 7, 4 " -dimethyl ether, tectochrysin, acylated flavone and glucosides components with their specific activity. Serpyllin, which comes under the category of flavone, has been isolated from A. serpyllifolia and its structure has been determined as 5-hydroxy-7, 8, 2′, 3′, 4′- pentamethoxyflavone. "
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    ABSTRACT: Andrographis is an important genus of the family Acanthaceae known for its ethnomedicinal claims and for a variety of medicinal properties. The current review briefly summarizes the distribution, phytochemical components, biological and pharmacological activities of Andrographis spp. distributed in Southern Eastern Ghats, South India along with colour photographs of important species. More than 10 species of Andrographis are distributed in Southern Eastern Ghats, South India which includes A. affinis, A. alata, A. beddomei, A. elongata, A. echioides, A. glandulosa, A. lineata, A. ovata, A. paniculata and A. serpyllifolia. Among the different species of Andrographis, only some are studied for their biological and pharmacological activities. The phytochemical components in Andrographis spp. responsible for the biological and pharmacological activities reported in various studies are andrographolide, neoandrographolide, 14-deoxy- 11,12-didehydroandrographolide, 14-deoxy-andrographolide, andrographiside and serpyllin. The important pharmacological effects of Andrographis spp. and their derivatives include the activities like antidiabetic, anticancer, antifertility, anti-inflammatory, antioxidant, antivenom, hepatoprotective, immunomodulatory, antimicrobial, antipyretic, anthelmintic and antiviral (against chikungunya) activity.
    Full-text · Article · Sep 2015
    • "It suppressed in vitro and ex vivo nitric oxide production when macrophages were collected after oral administration and subjected to lipopolysaccharides stimulation (Shen et al., 2002; Batkhuu et al., 2002). Furthermore , neoandrographolide was reported to inhibit prostaglandin E2 synthesis and TNF-α production, as well as to suppress dimethylbenzene-induced ear edema and acetic acid-induced vascular permeability (Liu et al., 2007a, 2007b). Andrograpanin, a hydrolysate from neoandrographolide, was also found to reduce NO, TNF-α, and IL-6 production in lipopolysaccharide (LPS)-activated macrophage cells derived from bone marrow in mice, possibly because of the downregulation of p38 MAPKs signaling pathways (Liu et al., 2008). "
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    ABSTRACT: Hepatic disease is one of the main causes of death in the world and occurs commonly induced by alcohol, viral and drugs. Treatment of hepatic diseases by using synthetic drugs is lead to serious adverse effects. Due to that, herbal treatment has appeared as an alternative treatment with good value in treating hepatic diseases. Herbs have been used traditionally for treating hepatic diseases since the past centuries. Numerous phytochemicals from herbs have been found as hepatoprotective (ability to minimize the effect of hepatic diseases which eventually improve hepatic function) agents. Although there are reports from many researchers on the hepatoprotective agents in various herbs, these scientific data are scattered and no conclusive information especially for various induced hepatic diseases is achieved to date. The researchers just consider the phytochemicals in herbs with specific induced of hepatic diseases. This limits researches on mapping the hepatoprotective properties for various induced of hepatic diseases. Therefore, it is important to compile and study the hepatoprotective agents in herbs in order to provide a baseline for future research such as blending and new formulation of herbs. Phytochemicals such as silymarin, andrographolide, neoandrographolide, picroside, kutkoside, phyllanthin and glycyrrhizin and so on are known to act as hepatoprotective agents for treating hepatic diseases. This review focuses on the herbs and their mechanisms of phytochemical group as hepatopotective agents. It has been found that hepatoprotection agents generally exert multiple effects such as antioxidant, free radical scavenging, antiviral and anti-inflammatory properties. The vital mechanisms of hepatoprotection agents are probably due to the presence structure of flavonoids in herbal plants. The results of this study indicate that extracts of phytochemicals from certain herbs have good potentials for use in various induced of hepatic diseases.
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