Survey of the year 2005 commercial optical biosensor literature. J. Mol. Recognit. 19, 478-534

Center for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84132, USA.
Journal of Molecular Recognition (Impact Factor: 2.15). 11/2006; 19(6):478-534. DOI: 10.1002/jmr.808
Source: PubMed


We identified 1113 articles (103 reviews, 1010 primary research articles) published in 2005 that describe experiments performed using commercially available optical biosensors. While this number of publications is impressive, we find that the quality of the biosensor work in these articles is often pretty poor. It is a little disappointing that there appears to be only a small set of researchers who know how to properly perform, analyze, and present biosensor data. To help focus the field, we spotlight work published by 10 research groups that exemplify the quality of data one should expect to see from a biosensor experiment. Also, in an effort to raise awareness of the common problems in the biosensor field, we provide side-by-side examples of good and bad data sets from the 2005 literature.

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    • "Label-free biosensing devices can incorporate different specific recognition elements, such as antibodies, DNA molecules, or enzymes that convert the reaction with a given analyte into a quantifiable signal such as an optical, acoustic, electrochemical, or mass change [2,5]. Among several optical methods based on surface plasmon resonance, enhanced Raman scattering, wave guiding, Bragg diffraction, and photonic bandgaps, the reflective interference spectroscopy [RIfS] method is recognised as particularly promising for the development of label- free biosensing devices [6-10]. "
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    ABSTRACT: A study of reflective interference spectroscopy [RIfS] properties of nanoporous anodic aluminium oxide [AAO] with the aim to develop a reliable substrate for label-free optical biosensing is presented. The influence of structural parameters of AAO including pore diameters, inter-pore distance, pore length, and surface modification by deposition of Au, Ag, Cr, Pt, Ni, and TiO2 on the RIfS signal (Fabry-Perot fringe) was explored. AAO with controlled pore dimensions was prepared by electrochemical anodization of aluminium using 0.3 M oxalic acid at different voltages (30 to 70 V) and anodization times (10 to 60 min). Results show the strong influence of pore structures and surface modifications on the interference signal and indicate the importance of optimisation of AAO pore structures for RIfS sensing. The pore length/pore diameter aspect ratio of AAO was identified as a suitable parameter to tune interferometric properties of AAO. Finally, the application of AAO with optimised pore structures for sensing of a surface binding reaction of alkanethiols (mercaptoundecanoic acid) on gold surface is demonstrated.
    Full-text · Article · Jan 2012 · Nanoscale Research Letters
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    • "Previous experimental approaches have not provided a clear separation of individual processes, namely cell resistance, rigidity, and binding, and so have limited value in deciphering binding processes [11]. Techniques such as Scatchard analysis, Biacore, and shear plates have generated some thermodynamic and macroscopic values, providing , at best, what are termed the affinity, binding, or association constants [12] [13] [14] [15] [16] [17] [18]. This information is satisfactory up to a point, but it does not provide a satisfactory measurement of the forces of individual molecular interactions. "
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    ABSTRACT: Microbubble science is expanding beyond ultrasound imaging applications to biological targeting and drug/gene delivery. The characteristics of molecular targeting should be tested by a measurement system that can assess targeting efficacy and strength. Atomic force microscopy (AFM) is capable of piconewton force resolution, and is reported to measure the strength of single hydrogen bonds. An in-house targeted microbubble modified using the biotin-avidin chemistry and the CD31 antibody was used to probe cultures of Sk-Hep1 hepatic endothelial cells. We report that the targeted microbubbles provide a single distribution of adhesion forces with a median of 93pN. This interaction is assigned to the CD31 antibody-antigen unbinding event. Information on the distances between the interaction forces was obtained and could be important for future microbubble fabrication. In conclusion, the capability of single microbubbles to target cell lines was shown to be feasible with AFM.
    Full-text · Article · Oct 2010 · Colloids and surfaces B: Biointerfaces
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    • "Even though, all these biosensors are dependent on SPR mode, their utilities are based on the exploitation in biomolecular analysis with high sensitivity [38]. Exhaustive year-wise surveys on SPR biosensor were published by Myszka and his colleague [27] [28] [29] [30] [31] [32] [33] [34] [35] [36]. In the past, SPR biosensor has become an established method of measuring various biochem- Fig. 1. "
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    ABSTRACT: In the past, surface plasmons have been used to improve the surface sensitivity of several spectroscopic measurements. Surface plasmon resonance reflectivity measurements can be used to detect biomolecules and their interactions by changes in the local index of refraction upon adsorption of the target molecule to the metal surface. Using this principle several SPR-based biosensors have been generated in the past with wide range of applications in the field of biology, biomedicine and biochemistry. Among several techniques available for biomolecular interactions, SPR-based Biacore is one of the widely used real-time monitoring systems and proved to be a convenient system for wide range of molecular sizes from small ligands to whole cells. Biacore system can be employed for biomolecular interactions, selection procedures, epitope mapping, molecular discriminations, kinetic analyses, screening processes and other applications. There were several successes reported using the versatility of Biacore system. In this report, several biosensing applications of this system were overviewed.
    Full-text · Article · Oct 2010 · Sensors and Actuators B Chemical
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