Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins

UMR Biodiversité Gènes Communautés, INRA, Equipe de génétique, Cestas, France.
PROTEOMICS (Impact Factor: 3.81). 12/2006; 6(24):6509-27. DOI: 10.1002/pmic.200600362
Source: PubMed


Poplar is the first forest tree genome to be decoded. As an initial step to the comprehensive analysis of poplar proteome, we described reference 2-D-maps for eight tissues/organs of the plant, and the functional characterization of some proteins. A total of 398 proteins were excised from the gels. About 91.2% were identified by nanospray LC-MS/MS, based on comparison with 260,000 Populus sp. ESTs. In comparison, reliable PMFs were obtained for only 51% of the spots by MALDI-TOF-MS, from which 43% (83 spots) positively matched gene models of the Populus trichocarpa genome sequence. Among these 83 spots, 58% matched with the same proteins as identified by LC-MS/MS, 21.7% with unknown function proteins and 19.3% with completely different functions. In the second phase, we studied the effect of drought stress on poplar root and leaf proteomes. The function of up- and down-regulated proteins is discussed with respect to the physiological response of the plants and compared with transcriptomic data. Some important clues regarding the way poplar copes with water deficit were revealed.

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Available from: Francis Michel Martin, Jun 11, 2014
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    • "Plant acclimation to stress is associated with profound changes in leaf proteome, thus, proteomic studies can significantly contribute to unraveling the possible relationship between protein modifications and plant stress acclimation (Kosová et al. 2011), namely those that result from changes in the cell redox state. In recent years, several proteomic studies have been performed on various species under water deficit conditions such as poplar, oak, rice, sunflower and grapevine (Costa et al. 1998; Salekdeh et al. 2002; Hajheidari et al. 2005; Ali and Komatsu 2006; Jorge et al. 2006; Plomion et al. 2006; Vincent et al. 2007; Bonhomme et al. 2009b; Fulda et al. 2011; Sergeant et al. 2011) and it has been showed that proteomic analysis may reveal subtle differences in drought stress responses that are not evident from mRNA measurements (Hajheidari et al. 2005). However, to the best of our knowledge, this is the first report to study simultaneously the dynamics of the leaf proteome alongside photosynthesis, antioxidants and plant hormones in C. albidus shrubs growing in their natural habitat and subjected to long-term summer drought stress. "
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    ABSTRACT: Main conclusion The interaction between enzymatic and non-enzymatic antioxidants, endogenous levels of ABA and ABA-GE, the rapid recuperation of photosynthetic proteins under re-watering as well the high level of antioxidant proteins in previously drought-stressed plants under re-watering conditions, will contribute to drought resistance in plants subjected to a long-term drought stress under Mediterranean field conditions. This work provides an overview of the mechanisms of Cistus albidus acclimation to long-term summer drought followed by re-watering in Mediterranean field conditions. To better understand the molecular mechanisms of drought resistance in these plants, a proteomic study using 2-DE and MALDI-TOF/TOF MS/MS was performed on leaves from these shrubs. The analysis identified 57 differentially expressed proteins in water-stressed plants when contrasted to well watered. Water-stressed plants showed an increase, both qualitatively and quantitatively, in HSPs, and downregulation of photosynthesis and carbon metabolism enzymes. Under drought conditions, there was considerable upregulation of enzymes related to redox homeostasis, DHA reductase, Glyoxalase, SOD and isoflavone reductase. However, upregulation of catalase was not observed until after re-watering was carried out. Drought treatment caused an enhancement in antioxidant defense responses that can be modulated by ABA, and its catabolites, ABA-GE, as well as JA. Furthermore, quantification of protein carbonylation was shown to be a useful marker of the relationship between water and oxidative stress, and showed that there was only moderate oxidative stress in C. albidus plants subjected to water stress. After re-watering plants recovered although the levels of ABA-GE and antioxidant enzymes still remain higher than in well-watered plants. We expect that our results will provide new data on summer acclimation to drought stress in Mediterranean shrubs.
    Full-text · Article · Dec 2014 · Planta
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    • "RSS and RLS are both important components of Rubisco, the key enzyme involved in photosynthetic CO 2 assimilation (Caruso et al., 2009). There have been contrasting results in different studies on the regulation of Rubisco in response to drought stress, as some studies found upregulation (Zhou et al., 2011; Budak et al., 2013), some reported downregulation (Ali and Komatsu, 2006; Plomion et al., 2006), and some reported both up-and downregulation (Caruso et al., 2009). "
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    ABSTRACT: Drought stress occurring during the reproductive growth stage leads to considerable reductions in crop production and has become an important limiting factor for food security globally. In order to explore the possible role of drought priming (pre-exposure of the plants to mild drought stress) on the alleviation of a severe drought stress event later in development, wheat plants were subjected to single or double mild drought episodes (soil relative water content around 35–40%) before anthesis and/or to a severe drought stress event (soil relative water content around 20–25%) 15 d after anthesis. Here, single or double drought priming before anthesis resulted in higher grain yield than in non-primed plants under drought stress during grain filling. The photosynthesis rate and ascorbate peroxidase activity were higher while malondialdehyde content was lower in primed plants than in the non-primed plants under drought stress during grain filling. Proteins in flag leaves differently expressed by the priming and drought stress were mainly related to photosynthesis, stress defence, metabolism, molecular chaperone, and cell structure. Furthermore, the protein abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit, Rubisco activase and ascorbate peroxidase were upregulated in primed plants compared with non-primed plants under drought stress during grain filling. In conclusion, the altered protein expression and upregulated activities of photosynthesis and ascorbate peroxidase in primed plants may indicate their potential roles in alleviating a later-occurring drought stress episode, thereby contributing to higher wheat grain yield under drought stress during grain filling.
    Full-text · Article · Dec 2014 · Journal of Experimental Botany
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    • "The protocol for protein extraction from poplar leaves was based on the published protocols [30] [31] [32] [33] with some modifications . Fresh leaves were ground in liquid N 2 using a mortar and pestle. "

    Full-text · Dataset · Oct 2014
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