Prolonged Viral RNA Detection in Blood and Lymphoid Tissues from Coxsackievirus B4 E2 Orally-Inoculated Swiss Mice

Université du Droit et de la Santé Lille 2, Lille, Nord-Pas-de-Calais, France
Microbiology and Immunology (Impact Factor: 1.24). 02/2006; 50(12):971-4. DOI: 10.1111/j.1348-0421.2006.tb03874.x
Source: PubMed


The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT-PCR and semi-nested-RT-PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various postinfectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues.

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Available from: Mahjoub Aouni
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    • "In fact, a single inoculation of the mutant Sabin3-like by i.p route protected all mice challenged 16 days later with a virulent CVB3 and CVB4 E2 which induced, respectively, myocarditis and pancreatitis in the unprotected control mice. Heart and pancreatic damages due to viral CVB3 and CVB4 replication have been demonstrated to be clearly evident at 10 days post-infection (Tracy et al. 1992; Tu et al. 1995; Bopegamage et al. 2003, 2005; Jaidane et al. 2006). However, one mouse immunized with the attenuated Sabin3-like strain, developed pancreatitis after challenge by i.p route with CVB4 E2. "
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    • "CVB4 infection of murine fetal thymic organ cultures also interferes with T cell differentiation (Brilot et al., 2008). Outbred mice can be infected with CVB4 following oral inoculation, which results in systemic spreading of viral RNA and a prolonged detection of CVB4 RNA in thymus, spleen, and blood up to 70 days after inoculation (Jaïdane et al., 2006). Moreover, CVB4 infection of a murine mTEC line induces a dramatic decrease in Igf2 mRNA and IGF-2 protein in this cell line, while Igf1 transcripts were relatively unaffected. "
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