De Coppi, P. et al. Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotech. 25, 100-106

Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1094, USA.
Nature Biotechnology (Impact Factor: 41.51). 02/2007; 25(1):100-6. DOI: 10.1038/nbt1274
Source: PubMed


Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.

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Available from: Mohummad Minhaj Siddiqui, Apr 17, 2014
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    • "ERCs, an attractive novel type of adult MSCs isolated from menstrual blood, were capable of differentiating into 9 lineages[22]. As we all know, MSCs are a group of self-renewing, pluripotent stromal cells derived from tissues such as bone marrow[31], cord blood[32], adipose tissue[33]and amniotic fluid[34], and have been demonstrated that they could promote tissue repair and possess immunomodulatory and anti-inflammatory properties. ERCs express CD9, CD13, CD14, CD29, CD44, CD73, CD90, CD105, CD117, CD133, CD146[35], but not STRO-1, CD31 (endothelial) and CD34 (haemopoietic stem cell and endothelial)[36]. "
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    ABSTRACT: Endometrial regenerative cells (ERCs) is an attractive novel type of adult mesenchymal stem cells that can be non-invasively obtained from menstrual blood and are easily replicated at a large scale without tumorigenesis. We have previously reported that ERCs exhibit unique immunoregulatory properties in experimental studies in vitro and in vivo. In this study, the protective effects of ERCs on renal ischemia–reperfusion injury (IRI) were examined. Renal IRI in C57BL/6 mice was induced by clipping bilateral renal pedicles for 30 min, followed by reperfusion for 48 h. ERCs were isolated from healthy female menstrual blood, and were injected (1 million/mouse, i.v.) into mice 2 h prior to IRI induction. Renal function, pathological and immunohistological changes, cell populations and cytokine profiles were evaluated after 48 h of renal reperfusion. Here, we showed that as compared to untreated controls, administration of ERCs effectively prevented renal damage after IRI, indicated by better renal function and less pathological changes, which were associated with increased serum levels of IL-4, but decreased levels of TNF-α, IFN-γ and IL-6. Also, ERC-treated mice displayed significantly less splenic and renal CD4 + and CD8 + T cell populations, while the percentage of splenic CD4 + CD25 + regulatory T cells and infiltrating M2 macrophages in the kidneys were significantly increased in ERC-treated mice. This study demonstrates that the novel anti-inflammatory and immunoregulatory effects of ERCs are associated with attenuation of renal IRI, suggesting that the unique features of ERCs may make them a promising candidate for cell therapies in the treatment of ischemic acute kidney injury in patients.
    Full-text · Article · Dec 2016 · Journal of Translational Medicine
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    • "Postnatal sources of stem cells can be reached from different kinds of tissues including bone marrow, brain, adipose tissue, umbilical cord blood and amniotic fluid [2] [3] [4] [5] [6]. The umbilical cord is an extraembryonic structure that supplies feeding for the fetus during the intrauterine life. "
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    ABSTRACT: Wharton's Jelly-derived Mesenchymal stem cells (WJ-MSCs) have been found to be an alternative source of stem cells for the regenerative medicine. This is as WJ-MSCs have potential for differentiation, self-renewal and unique immunomodulatory properties. Aim of the work: This work was conducted to assess our lab and staff capabilities in our Tissue Culture and Medical Genetics Unit to isolate, culture, identify and characterize WJ-MSCs derived from the adult human umbilical cord to be used for clinical applications in the future. Material and Methods: WJ-MSCs were isolated from the human umbilical cord collected from late-gestation pregnancy after cesarean section. Isolated WJ-MSCs cultured as P0 and recultured as P1. After confluency, WJ-MSCs were collected and characterized for surface markers expression using flow cytometry technique. Results: WJ-MSCs isolated in this study were positive for CD44, CD90, CD105 and negative for CD34. These findings suggest that WJ-MSCs appeared to be readily obtainable and highly proliferative cell lines that come from noninvasive source. Conclusion: isolated WJ-MSCs may be a good noninvasive source for stem cell therapy and regenerative medicine in the future. However, further studies should be done to evaluate its use as an allogenic cell source and expression of other markers in relation to cell immunogenicity.
    Full-text · Article · Sep 2015
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    • "the mouse subretinal space, could lead to teratomas formation (Arnhold et al. 2004). Among extra-embryonic tissues, amniotic fluid may represent a suitable source of mesenchymal stem cells (MSCs), characterized by carrying minimal somatic mutations and by showing high differentiative potential (Orciani et al. 2011; De Coppi et al. 2007). These cells are able not only to differentiate into cells of mesenchymal lineages in response to specific culture conditions in vitro but also into endoderm-derived cells, such as hepatocytes and astrocytes , olygodendrocytes and neurons (Zagoura et al. 2012; Rennie et al. 2013; Orciani et al. 2011; Salvolini et al., 2011). "
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    ABSTRACT: Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this problem is lacking, though recently, it has been postulated the existence of a subpopulation of RPE stem cells (RPESCs) derived from adult RPE and able to reconstitute a functional RPE. On the other hand, the evidence related to the differentiative potential of human mesenchymal stem cells (MSCs) is continuously increasing. Among others, amniotic fluid-derived MSCs (AF-MSCs) may be a promising candidate, since these cells are characterized by high proliferation and differentiative potential. In this study, AF-MSCs and RPESCs were isolated, characterized to assay their stemness and induced to neuronal/retinal differentiation; specific RPE markers were then analyzed. Our results indicate that RPESCs are more suitable candidates for RPE replacement than AF-MSCs.
    Full-text · Article · Apr 2015 · Cell and Tissue Research
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