Article

A Mouse for All Reasons

Cell (Impact Factor: 32.24). 02/2007; 128(1):9-13. DOI: 10.1016/j.cell.2006.12.018
Source: PubMed

ABSTRACT

Three major mouse knockout programs are underway worldwide, working together to mutate all protein-encoding genes in the mouse using a combination of gene trapping and gene targeting in mouse embryonic stem (ES) cells. Although the current emphasis is on production of this valuable resource, there are significant efforts to facilitate program coordination, to enhance the availability of this resource, and to plan for future efforts in mouse genetics research.

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    • "Third, before the advent of next generation sequencing , attempts at saturation mutagenesis of the mouse genome with chemical mutagens were slow and expensive, requiring extensive breeding programmes to identify mutations [25]. Key research centres in the United States (US), continental Europe, the United Kingdom (UK), and Canada launched the IKMC effort, using gene targeting and, to lesser extent, gene trapping [26]. 1 The IKMC included the KnockOut Mouse Project (KOMP) funded by the National Institutes of Health (NIH, US), "
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    • "The arrows highlight features that are present in gene predictions from Ensembl and HAVANA/Vega but not from NCBI genome features represented at NCBI's Gene resource (Brown et al. 2015; Sayers et al. 2012) and was a primary source of genes for the first phase of The International Knockout Mouse Project (KOMP) (Bradley et al. 2012; International Mouse Knockout et al. 2007). The output from MGI's unified gene catalog process systematically identifies gene models that are potentially problematic in their structural details as well as those that appear to be equivalent across different sources but have contradictory biotype annotations. "
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    • "The identity of cells can be altered by the forced induction of combination of transcription factors (TFs) (Takahashi and Yamanaka, 2006; Vierbuchen et al., 2010; Ieda et al., 2010; Sekiya and Suzuki, 2011; Huang et al., 2011; Hiramatsu et al., 2011), the forced induction of single TFs (Davis et al., 1987; Nishiyama et al., 2009; Correa-Cerro et al., 2011; Yamamizu et al., 2013) or by the repression of single TFs (Skarnes et al., 2004; Ivanova et al., 2006; Collins et al., 2007; Nishiyama et al., 2013). "
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