Article

Picobiliphytes: A Marine Picoplanktonic Algal Group with Unknown Affinities to Other Eukaryotes

Department of Biology, Laval University, Quebec City, Quebec, Canada
Science (Impact Factor: 33.61). 02/2007; 315(5809):253-5. DOI: 10.1126/science.1136264
Source: PubMed

ABSTRACT

Environmental sequencing has revealed unimagined diversity among eukaryotic picoplankton. A distinct picoplanktonic algal
group, initially detected from 18S ribosomal DNA (rDNA) sequences, was hybridized with rRNA λ-targeted (rRNA-targeted) probes, detected by tyramide signal amplification–fluorescent
in situ hybridization, and showed an organelle-like body with orange fluorescence indicative of phycobilins. Using this fluorescence
signal, cells were sorted by flow cytometry and probed. Hybridized cells contained a 4′,6′-diamidino-2-phenylindole–stained
organelle resembling a plastid with a nucleomorph. This suggests that they may be secondary endosymbiotic algae. Pending the
isolation of living cells and their formal description, these algae have been termed picobiliphytes.

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    • "icomo - nas , which should stabilise trees further ( Roure et al . , 2013 ) , and for a rappemonad as a deep - branching outgroup , as would obtaining completer transcriptomes or genome sequences . Environmental DNA sequences show that telonemids and picomonads are deep clades with numerous lineages available for culturing ( Bråte et al . , 2010 ; Not et al . , 2007 ; Seenivasan et al . , 2013 ; Shalchian - Tabrizi et al . , 2007 ; Yoon et al . , 2011 ) ; there is at least another undescribed microhelid ( Yabuki et al . , 2012 ) , but more important is to obtain Heliomorpha to break the endohelean branch more deeply ."
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