Article

Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5

Centro de Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário, Caixa Postal 354, 96010-900, Pelotas, RS, Brazil.
Veterinary Immunology and Immunopathology (Impact Factor: 1.54). 03/2007; 116(1-2):79-84. DOI: 10.1016/j.vetimm.2007.01.003
Source: PubMed

ABSTRACT

Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines.

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Available from: Fabrício Souza Campos, Oct 30, 2015
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    • "The composition of the propolis depends upon the time, vegetation, and the area of collection [9]. Propolis has several biological and pharmacological properties, as antimicrobial [10], anti-inflammatory [11], antioxidant [12] [13] [14] [15], antiparasitic [16], immune modularity and immune stimulant effects and it increased the percentage of protected animals suggesting its use in vaccines as an adjuvant [17] [18]. A reasonable approach to control and eliminate pasteurellosis in rabbits is to develop an improved vaccine as the current experimental vaccines do not provide a complete protection [19]. "

    Full-text · Dataset · Feb 2014
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    • "The composition of the propolis depends upon the time, vegetation, and the area of collection [9]. Propolis has several biological and pharmacological properties, as antimicrobial [10], anti-inflammatory [11], antioxidant [12] [13] [14] [15], antiparasitic [16], immune modularity and immune stimulant effects and it increased the percentage of protected animals suggesting its use in vaccines as an adjuvant [17] [18]. A reasonable approach to control and eliminate pasteurellosis in rabbits is to develop an improved vaccine as the current experimental vaccines do not provide a complete protection [19]. "
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    ABSTRACT: The present study was conducted to study the protective effect of ethanolic extract of propolis given subcutaneously (S/C) either alone or in combination with inactivated formalized Pasteurella multocida (P. multocida) vaccine in rabbits challenged with virulent P. multocida strain. Twenty-eight New-Zealand rabbits, 6-8 weeks old and not vaccinated against pasteurellosis, were randomly divided into four equal groups. Group (1) was kept as nonvaccinated control. Group (2) was injected S/C with propolis. Group (3) was vaccinated (S/C) with P. multocida vaccine only. Group (4) was injected with vaccine mixed with propolis as adjuvant. Groups (2, 3, and 4) received the same doses of propolis and vaccine after 4 weeks as a booster dose. The experiment continued for six weeks during which clinical signs, body weight, and mortality rate were recorded. Blood samples were collected every 2 weeks of treatment for evaluating the erythrogram and biochemical parameters. At the end of six weeks, all groups were subjected to challenge with a virulent strain of P. multocida. Two weeks later, tissue specimens were collected from different organs for histopathological investigation. Results showed that before challenge all rabbits of different groups were apparently healthy and had good appetite. After challenge, control group (1) showed acute form of the disease, 100% mortality rate, and severe histopathological changes. Rabbits of groups (2 and 3) showed less severe clinical signs, mortality rate, and histopathological changes than control. Rabbits of group (4) were apparently healthy with normal histological picture. In conclusion, an ethanolic extract of propolis injected alone or combined with formalized inactivated P. multocida vaccine improved general health conditions, liver and kidney functions in addition to reduction of the severity of adverse clinical signs, mortality rates, and histopathological changes associated with challenge of rabbits with P. multocida strain.
    Full-text · Article · Aug 2013
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    • "When administered together with aluminum hydroxide, the propolis extract increased both cellular and humoral responses [60]. A combination of an inactivated oil vaccine against bovine herpes virus type 5 and propolis extract also increased the humoral immune response in cattle [61]. Fractions of green propolis were tested against SuHV-1. "
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    ABSTRACT: Propolis is a bee product, composed mainly of plant resins and beeswax, therefore its chemical composition varies due to the geographic and plant origins of these resins, as well as the species of bee. Brazil is an important supplier of propolis on the world market and, although green colored propolis from the southeast is the most known and studied, several other types of propolis from Apis mellifera and native stingless bees (also called cerumen) can be found. Propolis is usually consumed as an extract, so the type of solvent and extractive procedures employed further affect its composition. Methods used for the extraction; analysis the percentage of resins, wax and insoluble material in crude propolis; determination of phenolic, flavonoid, amino acid and heavy metal contents are reviewed herein. Different chromatographic methods applied to the separation, identification and quantification of Brazilian propolis components and their relative strengths are discussed; as well as direct insertion mass spectrometry fingerprinting. Propolis has been used as a popular remedy for several centuries for a wide array of ailments. Its antimicrobial properties, present in propolis from different origins, have been extensively studied. But, more recently, anti-parasitic, anti-viral/immune stimulating, healing, anti-tumor, anti-inflammatory, antioxidant and analgesic activities of diverse types of Brazilian propolis have been evaluated. The most common methods employed and overviews of their relative results are presented.
    Full-text · Article · Jun 2011 · Chemistry Central Journal
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