Linezolid Alone and in Combination with Rifampicin Prevents Experimental Vascular Graft Infection Due to Methicillin-Resistant Staphylococcus aureus and Staphylococcus epidermidis

Department of Cardiovascular Surgery, School of Medicine, Pamukkale University, Denizli, Turkey.
Journal of Surgical Research (Impact Factor: 1.94). 06/2007; 139(2):170-5. DOI: 10.1016/j.jss.2006.10.003
Source: PubMed


In this report we describe the in vivo antibacterial activity of linezolid in an experimental graft infection model in rats and compare it with teicoplanin. The objective of this study was also to determine the effects of the interaction of linezolid when it was combined with rifampicin and test this effect against strains of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.
Graft infections were established in the subcutaneous tissue of 130 Wistar rats by implantation of Dacron grafts followed by a topical inoculation with 2 x 10(7) CFU of clinical isolates of MRSA and MRSE. The study included a control group and six groups for each of the staphylococcal strains: an inoculated group that did not receive any antibiotic prophylaxis, two inoculated groups that received intraperitoneal prophylaxis with teicoplanin or linezolid alone, an inoculated group that received rifampicin-soaked grafts, and two inoculated groups that received a combination prophylaxis consisting of intraperitoneal teicoplanin or linezolid and rifampicin-soaked grafts.
There was a reduction in the quantitative bacterial graft cultures in all prophylaxis groups when compared with inoculated control groups. There was not a statistically significant difference between linezolid and teicoplanin prophylaxis groups. The best results were obtained by a combination of rifampicin-soaked grafts with linezolid or teicoplanin.
We found no evidence to suggest that linezolid differs from teicoplanin regarding effectiveness in the prevention of prosthetic vascular graft infection. Linezolid plus rifampicin and teicoplanin plus rifampicin are demonstrated to be valuable prophylactic regimens.

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    • "Although linezolid-nonsusceptible strains are unusual [17], long courses of oxazolidinone therapy could select resistant mutants [18, 32]; hence, the use of a combined strategy might be considered in clinical practice. To date, the efficacy of linezolid as part of a combination has been studied against MRSA strains, but very few data has been reported against hGISA or GISA strains [19, 20]. Linezolid plus β-lactams exhibited bactericidal and synergistic activity against MRSA and hGISA strains in experimental models of endocarditis and meningitis [19]. "
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