Article

Prevalence of Cryptosporidium Infection in Cattle in Isfahan, Iran

Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Journal of Eukaryotic Microbiology (Impact Factor: 3.22). 02/2007; 54(1):100-2. DOI: 10.1111/j.1550-7408.2006.00236.x
Source: PubMed

ABSTRACT

Cryptosporidium are commonly identified as intestinal pathogens in humans and animals. Fecal samples from 480 cattle randomly selected from 30 regions in Isfahan, Iran, were examined to investigate the prevalence of Cryptosporidium infection. Cryptosporidium oocysts were identified by using sheather's concentration and the Ziehl-Neelsen modified staining technique in 30 of 480 cattle ranging from less than 6 mo of age to older than 1 yr of age. Infected animals were found in 86.6% (26/30) of regions investigated. Overall prevalence of infection was 6.25%, but higher in cattle less than 6 mo of age (10.8%) and this was statistically significant (P<0.05). Both sexes of cattle were infected with Cryptosporidium parasites, but prevalences were higher in diarrheic (56.7%) than in non-diarrheic (39%) cattle. Cryptosporidium appears to be prevalent in cattle in Isfahan.

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Available from: Mehdi Azami
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    • "The intestinal species C. bovis and C. ryanae are considered to be predominant in post-weaned calves (2–11 months) (Santin et al., 2004; Fayer et al., 2006). Previous studies of bovine cryptosporidiosis in Iran have been mostly microscopic examinations (Nouri and Toroghi , 1991; Azami, 2007), although studies of its molecular characterization have been conducted recently (Azami et al., 2007; Meamar et al., 2007; Fallah et al., 2008; Pirestani et al., 2008). Because of difficulty in differentiating species of Cryptosporidium based on morphological characteristics of the oocyst, we carried out the present study to measure the prevalence of bovine cryptosporidiosis and their variability with host age, and by using molecular techniques, to identify the species and genotypes of Cryptosporidium in Qazvin province, Iran. "

    Full-text · Dataset · Nov 2013
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    • "The prevalence (59.1%) of Cryptosporidium spp. in animals obtained in this study was compared with other studies including 6.2% of cattle in Isfahan (10), 35.3% of turkey in the north and west provinces (11), 18.9% of cattle in Kerman (12), and 18.8% of cattle in Qazvin (13). This information indicated that the prevalence of Cryptosporidium in this area is higher than in other parts of Iran. "
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    ABSTRACT: Cryptosporidium spp. is a coccidian parasite infected humans and animals. Prevalence rate of Cryptosporidium spp. infection associated with is some parameters such as sampling, age, season, country and contact to domestic animals. This study aimed to determine Cryptosporidium spp. Infection in humans and some animals in rural areas of Shushtar district from Khuzestan Province, south- west of Iran. In this study, Stool specimens were randomly collected from 45 cattle, 8 buffalos, 35 calves, 22 turkeys, 3 sheep, 2 geese as well as 62 humans in different seasons selected from rural areas of Shushtar district located in Khuzestan in the south- west of Iran from August 2009 to April 2011. The collected stool samples were examined by modified Ziehl-Neelsen staining method. Altogether, 68/115 (59.1%) domestic animals and 9/62 (14.5%) of humans were showed Cryptosporidium spp. infection in the study areas. In this study we found the high frequency of Cryptosporidium spp. infection in the studied areas.
    Full-text · Article · Mar 2012
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    • "The intestinal species C. bovis and C. ryanae are considered to be predominant in post-weaned calves (2–11 months) (Santin et al., 2004; Fayer et al., 2006). Previous studies of bovine cryptosporidiosis in Iran have been mostly microscopic examinations (Nouri and Toroghi , 1991; Azami, 2007), although studies of its molecular characterization have been conducted recently (Azami et al., 2007; Meamar et al., 2007; Fallah et al., 2008; Pirestani et al., 2008). Because of difficulty in differentiating species of Cryptosporidium based on morphological characteristics of the oocyst, we carried out the present study to measure the prevalence of bovine cryptosporidiosis and their variability with host age, and by using molecular techniques, to identify the species and genotypes of Cryptosporidium in Qazvin province, Iran. "
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    ABSTRACT: The aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of Cryptosporidium in cattle from 15 dairy farms in Qazvin province, Iran. Fecal samples, collected from 272 cattle during May 2006 to December 2007, were characterized microscopically. Oocysts from 51 positive samples were analyzed using PCR assay of 18S SSU rRNA, restriction fragment length polymorphism (RFLP) and sequencing. We identified 72.6% of the positive samples as Cryptosporidium parvum, 17.7% as Cryptosporidium andersoni, 7.8% as Cryptosporidium bovis and 1.9% as a novel genotype of C. parvum possessing a single mutation on MboII restriction. An infection rate of 19.5% of C. parvum among 174 pre-weaned calves was significantly higher than the 3.1% among 98 post-weaned calves (P<0.0006). This is the first report of C. bovis and the new subgenotype of C. parvum in Iranian cattle.
    Full-text · Article · Nov 2008 · Veterinary Parasitology
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