Amodiaquine Metabolism is Impaired by Common Polymorphisms in CYP2C8: Implications for Malaria Treatment in Africa

Department of Medicine, University of California, San Francisco, San Francisco, California, United States
Clinical Pharmacology &#38 Therapeutics (Impact Factor: 7.9). 09/2007; 82(2):197-203. DOI: 10.1038/sj.clpt.6100122
Source: PubMed


Metabolism of the antimalarial drug amodiaquine (AQ) into its primary metabolite, N-desethylamodiaquine, is mediated by CYP2C8. We studied the frequency of CYP2C8 variants in 275 malaria-infected patients in Burkina Faso, the metabolism of AQ by CYP2C8 variants, and the impact of other drugs on AQ metabolism. The allele frequencies of CYP2C8*2 and CYP2C8*3 were 0.155 and 0.003, respectively. No evidence was seen for influence of CYP2C8 genotype on AQ efficacy or toxicity, but sample size limited these assessments. The variant most common in Africans, CYP2C8(*)2, showed defective metabolism of AQ (threefold higher K(m) and sixfold lower intrinsic clearance), and CYP2C8(*)3 had markedly decreased activity. Considering drugs likely to be coadministered with AQ, the antiretroviral drugs efavirenz, saquinavir, lopinavir, and tipranavir were potent CYP2C8 inhibitors at clinically relevant concentrations. Variable CYP2C8 activity owing to genetic variation and drug interactions may have important clinical implications for the efficacy and toxicity of AQ.


Available from: Jean-Bosco Ouédraogo, Apr 26, 2014
Amodiaquine Metabolism is Impaired by
Common Polymorphisms in CYP2C8:
Implications for Malaria Treatment in Africa
S Parikh
, J-B Ouedraogo
, JA Goldstein
, PJ Rosenthal
and DL Kroetz
Metabolism of the antimalarial drug amodiaquine (AQ) into its primary metabolite, N-desethylamodiaquine, is mediated
by CYP2C8. We studied the frequency of CYP2C8 variants in 275 malaria-infected patients in Burkina Faso, the
metabolism of AQ by CYP2C8 variants, and the impact of other drugs on AQ metabolism. The allele frequencies of
CYP2C8*2 and CYP2C8*3 were 0.155 and 0.003, respectively. No evidence was seen for influence of CYP2C8 genotype on
AQ efficacy or toxicity, but sample size limited these assessments. The variant most common in Africans, CYP2C8*2,
showed defective metabolism of AQ (threefold higher K
and sixfold lower intrinsic clearance), and CYP2C8*3 had
markedly decreased activity. Considering drugs likely to be coadministered with AQ, the antiretroviral drugs efavirenz,
saquinavir, lopinavir, and tipranavir were potent CYP2C8 inhibitors at clinically relevant concentrations. Variable CYP2C8
activity owing to genetic variation and drug interactions may have important clinical implications for the efficacy and
toxicity of AQ.
Malaria, in particular that caused by Plasmodium falciparum,
remains among the leading causes of morbidity and mortality
in the developing world.
Recent estimates suggest that more
than 500 million episodes of P. falciparum malaria occurred
in 2002, leading to one to three million deaths.
The burden
of malaria is heaviest in sub-Saharan Africa, where resistance
to the most commonly employed antimalarials, in particular
chloroquine and sulfadoxine–pyrimethamine (PYR), is wide-
spread. In addition, given the level of transmission in many
areas, individuals may receive several short courses of
antimalarial therapy every year.
To combat the emergence
and spread of resistance, the World Health Organization
(WHO) has recommended the use of combination anti-
malarial therapy for P. falciparum malaria. Amodiaquine
(AQ), a 4-aminoquinoline similar in structure to chloro-
quine, is included in two of five recommended regimens,
AQ þ artesunate and AQ þ sulfadoxine–PYR.
In addition,
AQ monotherapy is still widely used to treat malaria in
AQ was introduced as an antimalarial in the 1940s. In the
mid-1980s, AQ administration increased largely owing to
increased prophylactic use in Western travelers. However,
several reports soon emerged suggesting an unacceptable level
of toxicity of AQ, in particular agranulocytosis (estimated
1:2,100 users with a fatality rate of 1:31,000)
hepatotoxicity (1:15,600 with numerous fatalities).
commendations for chemoprophylaxis with AQ were
dropped, and the WHO removed AQ from its Essential
Drugs List in 1990.
However, subsequent review has
suggested that AQ toxicity was primarily seen in non-
Africans receiving long-term chemoprophylaxis and the drug
was reinstated in 1996 by the WHO as an option for treating
malaria, with millions of dosages subsequently given each
The metabolism of AQ has been characterized in studies
using human liver microsomes and recombinant enzymes.
AQ is metabolized to its primary metabolite, N-desethyla-
modiaquine (DEAQ), by the cytochrome P450 (CYP) 2C8
which accounts for 7% of the total microsomal
CYP content of the liver
and is estimated to carry out
oxidative metabolism of at least 5% of drugs.
relatively common sequence-altering variants are denoted as
nature publishing group
Received 10 November 2006; accepted 20 December 2006; published online 14 March 2007. doi:10.1038/sj.clpt.6100122
Department of Medicine, San Francisco General Hospital, University of California, San Francisco, USA;
Institut de Recherche en Science de la Sante, Bobo-
Dioulasso, Burkina Faso;
Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences/National Institutes of Health, Research
Triangle Park, North Carolina, USA;
Department of Biopharmaceutical Sciences, School of Pharmacy, University of California, San Francisco, USA. Correspondence:
S Parikh (
Page 1
CYP2C8*2, CYP2C8*3, and CYP2C8*4, with the wild-type
denoted as CYP2C8*1 (Table 1,
CYP2C8*2 is most prevalent in those of African
descent, whereas CYP2C8*3 is more prevalent among
Several other single nucleotide polymorphisms
have been described, including coding polymorphisms and
two translational stop codons in Japanese, but at much lower
Both CYP2C8*2 and CYP2C8*3 are
defective in metabolism of the anticancer agent paclitaxel,
and CYP2C8*3 has reduced activity towards the endogenous
substrate arachidonic acid.
The aim of this study is to assess
the impact of CYP2C8 polymorphisms on AQ response and
toxicity in a cohort of malaria infected patients in Burkina
Faso and to examine the in vitro metabolism of AQ by
CYP2C8 variants. Additionally, we investigate the potential
for significant CYP2C8 inhibition by other drugs likely to be
coadministered with AQ in sub-Saharan Africa.
A total of 280 subjects in the AQ monotherapy arm
completed the clinical trial and had efficacy outcomes; 275
of these subjects had DNA available and were genotyped for
three nonsynonymous CYP2C8 variants (Table 2). All
genotypes were in Hardy–Weinberg equilibrium. The allelic
frequency for CYP2C8*2 (805A4T) was 0.115 and 25% of
the population were heterozygotes. Only five (2%) indivi-
duals were homozygotes for the CYP2C8*2 variant. The
CYP2C8*3 allele (416G4A and 1196A4G) was much less
common in this African population (allele frequency 0.004).
The CYP2C8*4 (792C4 G) allele was not detected.
Association with treatment outcome and adverse events
Overall, 82.2% of participants in the AQ treatment arm of the
study responded successfully to treatment, with no evidence
of clinical malaria or parasitemia over 28 days of follow-up.
Efficacy outcomes did not vary between CYP2C8*1 homo-
zygotes and CYP2C8*2 heterozygotes (Table 3). In addition,
time to therapeutic failure did not vary between these two
groups (data not shown). All five CYP2C8*2 homozygotes
responded to therapy.
Adverse events were uncommon in the AQ monotherapy
There was an increase in the self-reported rate of
abdominal pain in both heterozygotes and homozygotes for
the variant CYP2C8*2 genotype compared with wild-type
genotype (52 vs 30%, Po0.01). No other associations were
seen between CYP2C8*2 genotype and specific adverse
events, including nausea, vomiting, fatigue, and jaundice.
AQ metabolism by recombinant CYP2C8 proteins
High-performance liquid chromatography (HPLC)/UV ana-
lysis for the metabolites of AQ after incubation with
recombinant CYP2C8 revealed a single metabolite. The
metabolite was identified as DEAQ by comigration with a
reference standard. Retention times for DEAQ, primaquine
diphosphate, and AQ were 14.8, 16.9, and 18.1 min,
respectively (Figure 1). Formation of DEAQ in CYP2C8
incubations was linear with time and protein concentration.
Under the assay conditions used in this study, metabolites
such as N-bisDEAQ, 2-hydroxyDEAQ, or the M2 metabo-
lite, were not seen. This is consistent with the available li-
terature suggesting that metabolites such as M2 are formed
extrahepatically and the lack of evidence that CYP2C8
can catalyze the formation of metabolites other than
Formation of DEAQ by CYP2C8*1 exhibited typical
Michaelis–Menten kinetics (Figure 2). For the wild-type
allele, CYP2C8*1, the apparent V
was 0.2370.09 mmol/
min/mmol P450, with a K
of 0.8170.23 mM. The corre-
sponding intrinsic clearance (defined as V
) was 0.30 l/
min/mmol P450. The CYP2C8*2 allele had a significantly
lower V
of 0.1670.06 mmol/min/mmol P450 (P ¼ 0.04)
and a threefold higher K
, 2.5571.06 mM (P ¼ 0.05). The
intrinsic clearance of AQ for CYP2C8*2 was sixfold lower
than that for CYP2C8*1 (0.05 vs 0.30 l/min/mmol P450,
Po0.01). Metabolic activity was not sufficient to estimate
Table 1 Major CYP2C8 alleles in Caucasians and Africans
Allele Location
CYP2C8*1 NA None None
CYP2C8*2 Exon 5 805A4T I269F
CYP2C8*3 Exon 3, Exon 8 416G4A, 1196A4G R139K, K399R
CYP2C8*4 Exon 5 792C4G I264M
NA, not available.
Table 2 CYP2C8 allele frequencies in this study and other
Allele N CYP2C8*2 CYP2C8*3 CYP2C8*4 Ref
Burkina Faso 275 0.115 0.004 Not found This study
Zanzibar 165 0.139 0.021 0.006
Northern Ghana 200 0.168 Not found Not found
African Americans 82 0.183 0.018 Not found
Portugal 164 0.012 0.198 0.064
British Caucasians 116 0.004 0.150 0.075
Sweden 1468 Not reported 0.095 Not reported
Malaysian Indians 123 0.008 0.12 Not found
Japanese 360 Not found Not found Not found
Table 3 Association of CYP2C8*2 with treatment outcome and
adverse events
CYP2C8*2 genotype, n (%)
Outcome AA (n=199)
AT (n=67)
TT (n=5)
ACPR 164 (82) 57 (85) 5 (100)
Recrudescence 20 (10) 6 (9) 0
New infection 15 (7.5) 4 (6) 0
ACPR, adequate clinical and parasitological response.
Parasite genotyping results
were not obtained for four specimens.
198 VOLUME 82 NUMBER 2 | AUGUST 2007 |
Page 2
adequately the kinetic parameters for CYP2C8*3, as no
metabolism was detectable until AQ concentrations of
15–25 m
M were tested and solubility prevented measurements
within the saturable range for this enzyme.
Interactions between recombinant CYP2C8 and other drugs
We studied six drugs for their impact on the AQ
N-desethylase activity of recombinant CYP2C8 proteins
(Table 4). Trimethoprim (TMP), a widely used dihydrofolate
reductase inhibitor, inhibited CYP2C8 (IC
¼ 40.67
12.7 m
M). These results correlated with previous reports.
PYR, another dihydrofolate reductase inhibitor, also inhibited
CYP2C8 at similar concentrations (IC
¼ 45.1712.2 mM).
Efavirenz, an antiretroviral non-nucleoside reverse transcrip-
tase inhibitor that is widely used to treat human immuno
deficiency virus (HIV) infection, was a potent inhibitor of
CYP2C8 at clinically relevant concentrations (IC
¼ 4.07
2.5 m
M). HIV-1 protease inhibitors were also potent inhibitors
of CYP2C8-mediated AQ desethylase activity: saquinavir,
lopinavir, and tipranavir all inhibited the metabolism of AQ
at clinically relevant concentrations.
Few studies are available on the impact of genetic variation
on the metabolism of commonly used antimalarial drugs. We
describe the influence of CYP2C8 polymorphisms on the
metabolism of AQ. In our study population in Burkina Faso,
the variant allele CYP2C8*2 was common, with a prevalence
of 11.5% and the variant most prevalent in Caucasians,
CYP2C8*3, was rare.
These data correlate well with other
published allelic frequencies from West Africa (Table 2).
Compared with the wild-type enzyme, CYP2C8*2 showed a
threefold higher K
and sixfold lower intrinsic clearance for
AQ. These results are consistent with but of greater
magnitude than the twofold higher K
of recombinant
CYP2C8*2 for paclitaxel and the twofold increase in intrinsic
clearance for that substrate.
The decreased AQ desethylase
activity of the CYP2C8*3 variant was more profound than
that of the CYP2C8*2 variant, suggesting that effects of
reduced metabolism of AQ will be most pronounced in
CYP2C8*3 carriers. Indeed, no appreciable metabolism by
CYP2C8*3 was detectable until AQ substrate concentrations
nearly 15–20-fold higher than the K
of the wild-type
enzyme were tested. CYP2C8*3 also had extremely low
turnover for paclitaxel (6% of that of wild-type CYP2C8*1)
and a threefold lower turnover number for arachidonic acid.
In vivo studies have shown a 4.5-fold increase in the half-life
of (R)-ibuprofen in subjects homozygous for the CYP2C8*3
Absorbance (UV 340 nm)
10 15 20
Retention time
Figure 1 Chromatographic separation of (1) DEAQ, (2) primaquine internal
standard, and (3) AQ. This chromatogram shows the product formation from
an incubation of AQ with recombinant CYP2C8*1 protein.
Metabolite formation
(mol/min/mol P450)
[AQ], molar
Figure 2 Plot of velocity vs AQ concentration for the formation of
desethylamodiaquine by recombinant CYP2C8*1 and CYP2C8*2 proteins.
Each point is the mean velocity from triplicate determinations at a given
concentration and the lines were drawn using the estimated
Michaelis–Menten parameters.
Table 4 IC
values for inhibitors of recombinant CYP2C8
Systemic concentration (mM)
Drug IC
(mM)7SE C
Trimethoprim 40.6712.7 2
Pyrimethamine 45.1712.2 2.4
Efavirenz 4.072.5 12.9
Nevirapine b30
21.6 14
Saquinavir 1.870.8 5.5
Lopinavir 4.170.6 15.6
Tipranavir 2.170.3 78–95
Ritonavir 3.0371.14
15.5 5.1
Nelfinavir B40
6 3.3
NA, not available; SE, standard error. IC
data are means7SD from four
experiments. Serum concentrations are from published information. C
and C
are the mean maximum and minimum serum levels achieved under standard dosing
intervals, respectively.
On the basis of trimethoprim 160 mg q.i.d. dosing in HIV-
infected patients.
29 b
On the basis of pyrimethamine 750 mg single dose.
30 c
On the
basis of efavirenz 600 mg q.i.d. in HIV-infected individuals (Brystol–Myers Squibb
prescribing information).
On the basis of nevirapine 200 mg b.i.d. in HIV-1-infected
and Walsky et al.
32 e
On the basis of saquinavir 1200 mg t.i.d (as free
base) in HIV-infected individuals, and coadministered saquinavir soft gel capsule.
1,000 mg/ritonavir 100 mg b.i.d. in HIV-infected individuals (Roche prescribing
33 f
On the basis of lopinavir 400 mg/ritonavir 100 mg b.i.d. in HIV-
infected individuals (Abbott prescribing information).
On the basis of tipranavir
500 mg/ritonavir 200 mg b.i.d. in HIV-infected individuals (Boehringer Ingelheim
Pharmaceuticals prescribing information).
On the basis of ritonavir 600 mg b.i.d. in
healthy and HIV-infected individuals (Abbott prescribing information) and Walsky
et al.
32 i
On the basis of nelfinavir mesylate 1250 mg b.i.d. in HIV-infected individuals;
was determined before morning dosage (Agouron prescribing information) and
Walsky et al.
Page 3
allele compared with the CYP2C9*1 allele.
Our results for
AQ metabolism by CYP2C8 variants and the reported
findings for paclitaxel, arachidonic acid, and ibuprofen
illustrate that the magnitude of the effect of these
polymorphisms depends on the substrate and the relation-
ship of substrate concentration to K
AQ is predominantly metabolized into a single major
metabolite, DEAQ.
Several other metabolites have been
described, but were not identified in our studies (Figure 3).
Metabolism into DEAQ occurs rapidly, with no AQ
detectable within a few hours of oral intake (terminal half-
life, 5.2 h).
In contrast, DEAQ is present in the blood for
an extended period after therapy, with a terminal half-life of
9–18 days and wide interindividual variability in plasma
Both AQ and DEAQ have antimalarial activity, but
AQ is up to threefold more potent.
Nonetheless, owing to
its much higher concentrations, DEAQ is considered the
major active component.
Given our findings of impaired
conversion of AQ into DEAQ by the CYP2C8*2 and
CYP2C8*3 variants, it would be predicted that for both
variants AQ and DEAQ, concentration–time profiles would
be significantly altered, possibly contributing to the observed
pharmacokinetic variability.
However, it is not possible that
such alterations in AQ metabolism would affect therapeutic
efficacy, as both the parent drug and metabolite are active.
Indeed, in our study no impact on antimalarial efficacy was
demonstrated for the common CYP2C8*2 variant.
Of possibly greater importance than impacts on ther-
apeutic efficacy are potential effects of CYP2C8 variants on
AQ toxicity. As noted above, long-term usage of AQ for
malaria chemoprophylaxis led to important risks of blood
dyscrasias and hepatic disorders.
Mechanisms of AQ
toxicity are not known, but both AQ and DEAQ led to
inhibitory effects on bone marrow progenitor cells and
neutrophil function in vitro.
Additionally, several lines of
evidence suggest that AQ toxicity is mediated through the
production of an immunologically reactive quinonei-
a finding supported by the demonstration of
anti-AQ antibodies in individuals with AQ-associated
Importantly, DEAQ is less readily activated to
immunologically reactive compounds compared with
One would thus expect that individuals with
impaired metabolism of AQ into DEAQ may be at increased
risk of toxicity because of higher levels of AQ. We propose
that the severe toxicity seen with AQ usage as a chemopro-
phylactic may have been influenced by a relatively high
prevalence of the functionally defective CYP2C8*3 variant in
Caucasians (15–20%). Although the enzymatic defect with
the CYP2C8*2 allele prevalent in Africa (15–18% frequency)
is less profound than that for CYP2C8*3, individuals with the
variant gene might experience decreased tolerability and
increased toxicity with AQ. It is reassuring that our study did
not identify common toxicities or diminished tolerability for
AQ in CYP2C8*2 variants. Nonetheless, as AQ is now widely
used, with individuals probably receiving repeated courses of
therapy, careful monitoring of larger samples for adverse
events related to AQ and for associations with the CYP2C8*2
genotype are warranted.
(3× more active than
DEAQ, terminal t
5 h)
terminal t
>100 h
N -bisDEAQ
(Churchill, 1985;
Mount DL 1986;
Jewell H, 1995)
(Li Q, 2002)
(Churchill, 1985;
Mount DL, 1986;
Jewell H, 1995)
More readily formed by amodiaquine
Protein conjugation/
cell haptenization
(Harrison 1992; Jewell 1995)
cell hapten
Immune-mediated toxicity
Figure 3 Metabolic pathway for AQ showing the formation of the active DEAQ, other minor metabolites, and the reactive quinoneimine implicated in
immunotoxicity of AQ.
200 VOLUME 82 NUMBER 2 | AUGUST 2007 |
Page 4
We evaluated the impact of several drugs on CYP2C8-
mediated AQ metabolism. TMP is a component of
TMP–sulfamethoxasole, which is widely used to prevent
secondary infections in HIV-infected individuals and recom-
mended for daily usage in all HIV-1-infected Africans by
some authorities.
The related dihydrofolate reductase
inhibitor, PYR, is coformulated with sulfadoxine and
commonly administered with AQ to treat malaria.
In our
study, both TMP and PYR inhibited CYP2C8, but only at
concentrations not achieved by standard dosing of these
drugs. Of particular interest for interactions with AQ are
antiretroviral drugs that are increasingly available to treat the
more than 25 million HIV-infected individuals in Africa.
Considering non-nucleoside reverse transcriptase inhibitors,
nevirapine did not appreciably inhibit CYP2C8,
efavirenz was a potent inhibitor, with an IC
below the
mean minimum serum levels achieved under standard dosing
intervals (C
) achieved with standard dosing. Considering
antiretroviral protease inhibitors, previous studies showed
that ritonavir (at high doses), but not nelfinavir, were potent
CYP2C8 inhibitors.
In our study, saquinavir, lopinavir, and
tipranavir were potent CYP2C8 inhibitors at clinically
relevant concentrations. In particular, tipranavir had an
against CYP2C8 approximately 20-fold below the
minimum serum concentration achieved during standard
dosing. Given the tremendous burden of HIV–malaria
coinfection in Africa, such drug interactions are an important
In addition, the inhibition of CYP2C8 by
efavirenz, saquinavir, lopinavir, and tipranavir has implica-
tions not only for AQ administration but also for other
substrates of CYP2C8 such as paclitaxel, thiazolidinediones,
repaglinide, amiodarone, arachidonic acid, loperamide, and
It will be of interest to examine the potential
impact of antiretroviral–antimalarial interactions in clinical
We have described a more significant impact of a CYP2C8
variant on the metabolism of AQ than previously described
for any drug. These findings may explain, at least in part, AQ-
associated toxicity seen with long-term chemoprophylactic
usage in primarily Caucasian populations and they suggest
that differences in AQ efficacy and toxicity may also be
associated with different CYP2C8 genotypes in Africans.
With the high prevalence of the CYP2C8*2 variant in Africa,
and with increasing use of multiple drugs that may affect
CYP2C8-mediated metabolism, additional study of the
metabolism of AQ and careful monitoring for AQ-associated
toxicity are warranted.
Chemicals. Amodiaquine dihydrochloride (AQ), quinine, PYR, and
dilauroylphosphatidylcholine were obtained from Sigma-Aldrich (St
Louis, MO). Primaquine diphosphate and TMP were obtained from
MP Biomedicals (Solon, OH). The antiretrovirals saquinavir (as free
base), lopinavir, tipranavir, and efavirenz were obtained through the
AIDS Reference and Reagent Program, Division of AIDS, NIAID,
, glucose-6-phosphate, and glucose-6-phos-
phate dehydrogenase were obtained from BD Biosciences Discovery
Labware (San Jose, CA). HPLC-grade acetonitrile was purchased
from Fischer Scientific (Hampton, NH). Recombinant rat CYP
reductase was obtained from Fengyun Xu (University of California,
San Francisco, CA).
Subjects and clinical study. Details of the clinical study have been
Briefly, residents of Bobo-Dioulasso, Burkina Faso
more than 6 months of age with uncomplicated falciparum malaria
were randomized to receive sulfadoxine–PYR, AQ, or AQ plus
sulfadoxine–PYR in 2004. For this substudy, only samples from
patients treated with AQ monotherapy were analyzed. Patients were
followed for 28 days and treatment outcomes were classified
according to WHO guidelines with parasite genotyping performed
to distinguish true failures (recrudescences) from new infections.
each follow-up visit study, clinicians assessed patients for adverse
events, defined as any untoward medical occurrence, following
International Conference on Harmonization guidelines.
The study
was approved by the institutional review boards of the University of
California, San Francisco and the Centre Muraz, Bobo-Dioulasso,
Burkina Faso. All research subjects or their parents or guardians
approved the use of clinical specimens for genetic testing.
CYP2C8 genotyping and sequencing. For genetic analysis, DNA was
extracted from filter paper with chelex. Genotyping for CYP2C8*2,
*3, and *4 variants was performed using predesigned primers and
probes for the TaqMan 5’nuclease allelic discrimination assay on an
ABI 7500 real-time polymerase chain reaction system (Applied
Biosystems, Foster City, CA). Variant alleles are listed in Table 1.
Reactions were carried out with the following protocol: 951C for
10 min, then 50 cycles at 921C for 15 s and 601C for 90 s. For
confirmation of TaqMan results, random samples were amplified
and sequenced. Polymerase chain reaction primers for sequencing
were as described
and their products were purified with ExoSAP-
IT before direct sequencing (GE Healthcare Bio-Sciences Corp.,
Piscataway, NJ).
Metabolism of AQ by recombinant CYP2C8. Recombinant wild-type
and variant CYP2C8 allelic proteins were expressed in Escherichia
coli and partially purified as described previously in the laboratory
of author JAG (National Institute of Environmental Health Sciences,
NC). To study enzyme activities, recombinant CYP2C8 proteins
(5 pmol) and rat NADPH–CYP reductase (4 pmol/pmol P450) were
reconstituted with dilauroylphosphatidylcholine (3 mg/10 pmol
P450) and incubated at room temperature for 3 min. The
reconstituted enzymes were then preincubated in 0.1
pH 7.4, containing AQ substrate for 5 min at 371C. Reactions were
initiated by the addition of 1.3 m
, 3.3 mM glucose-6-
phosphate, and 0.4 U/ml glucose-6-phosphate dehydrogenase in a
final volume of 250 m l, incubated at 371C for 15 min, and terminated
with the addition of 125 ml ice-cold acetonitrile and 1 m
primaquine internal standard. Samples were centrifuged at
14,000 g for 10 min, and supernatant was analyzed by HPLC. To
determine enzyme kinetics, AQ was studied at eight different
concentrations ranging from 0 to 100 m
M. All solvent concentrations
were maintained at o0.1%.
Inhibition of AQ metabolism by selected antibiotics and antiretrovir-
als. Supersomes from baculovirus-infected cells expressing human
CYP2C8 were from BD Biosciences. CYP2C8 (2.5 pmol) was pre-
incubated with inhibitor (TMP, PYR, saquinavir, lopinavir,
tipranavir, or efavirenz) and buffer (0.1
, pH 7.4) at 371C
for 5 min, AQ substrate was added (1 m
M, approximately the enzyme
) and incubated for an additional 5 min; the reaction was
initiated by the addition of NADP
, glucose-6-phosphate, and
glucose-6-phosphate dehydrogenase to a final reaction volume of
250 ml and incubated at 371C for 15 min. Reactions were terminated
Page 5
and extracted as described above, with the exception that 1 mM
quinine was used as an internal standard. Typically, IC
determinations were performed in triplicate at seven inhibitor
concentrations, ranging from 0 to 100 m
M. Inhibitor concentrations
were adjusted as needed to adequately span the IC
Analysis of AQ and metabolites. AQ and its major metabolites were
detected and quantified using HPLC with UV detection. The HPLC
system consisted of an Agilent 1100 Series System with an HP
G1311A quaternary pump, an HP G1322A vacuum degasser, an HP
G1314A UV/Vis detector, and an HP G1313A automated liquid
sampler. A reverse-phase Vydac C
column (4.6 250 mm, 10 mM
particle size) was used for analyte separation. The mobile phase
consisted of water with 0.1% trifluoroacetic acid (A) and 95%
acetonitrile with 0.08% trifluoroacetic acid (B). The gradient was
initiated and maintained at 15% B for 5 min, followed by a linear
gradient to 19% B over 20 min. Chromatography was carried out at
a flow rate of 1.0 ml/min and effluent was monitored at 340 nm.
Statistical analysis. All data points represent the means of triplicate
determinations. K
, V
, and IC
data were determined by
nonlinear regression analysis using Prism 4.0 (GraphPad software).
Kinetic data were analyzed using a paired t-test with two-tailed
significance value. Statistical associations between alleles and
treatment outcome or adverse events were assessed by w
We thank the clinical study teams and technicians in the dispensaries of
Colsama, Sarlafao, and Ouezzin-Ville and the study participants and their
parents/guardians. We also thank members of the Kroetz lab for their
assistance. Financial support for this work was provided by the National
Institutes of Health (NIH) (5K23AI060681 and GM61390) and in part by
the Intramural Research Program of the NIH, National Institute of
Environmental Health Sciences.
The authors declared no conflict of interest.
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  • Source
    • "Nevertheless, more AEs occurred in the AS/AQ compared to the AS/SP group, due perhaps to the amodiaquine component that has been known to cause serious side effects such as hepatitis, decrease mean absolute neutrophil count and induced neutropenia [51]. Moreover, AEs due to AQ may be associated with genetic polymorphisms in the CYP450 genes that have been shown to reduce CYPCC8 expression in sub-Saharan Africa, leading to the poor metabolizer phenotype [52,53]. Although most of the AEs were self-limiting and disappeared by day 14, further evaluation of the safety of the AS/AQ combination is necessary, since its undesirable side effects remain a problem to indigenous of malaria endemic countries. "
    Full-text · Article · Feb 2016
  • Source
    • "A study on AQ in Burkina Faso showed a significant increased rate of adverse events in both heterozygotes and homozygotes for the variant CYP2C8*2 genotypes compared with the wild-type genotype (52% vs 30%, P < 0.01) (Parikh et al., 2007). Moreover, the recombinant CYP2C8*2 enzyme showed a defective metabolism of AQ (3-fold higher K m and 6-fold lower intrinsic clearance than the functional enzyme) in vitro (Parikh et al., 2007). The paper from Paganotti et al. (2011) reported a statistically significant trend among the three different genotypes for carrying CQ-resistant P. falciparum parasites in Burkina Faso (38.1%, 50.0%, 54.2% for AA, AT, TT genotypes, respectively, P = 0.02), an observation that might be explained by the pharmacokinetic of CQ. "
    [Show abstract] [Hide abstract] ABSTRACT: Human cytochrome P450 2C8 is a highly polymorphic gene and shows variation according to ethnicity. The CYP2C8*2 is a slow drug metabolism allele and shows 10–24% frequency in Black populations. The objective of this study was to assess the prevalence of CYP2C8*2 allele in Botswana among the San (or Bushmen) and the Bantu ethnic groups. For that purpose we recruited 544 children of the two ethnicities in three districts of Botswana from primary schools, collected blood samples, extracted DNA and genotyped them through PCR-based restriction fragment length polymorphism analysis. The results demonstrated that in the San the prevalence of the CYP2C8*2 allele is significantly higher than among the Bantu-related ethnic groups (17.5% and 8.5% for San and Bantu, respectively; P = 0.00002). These findings support the evidence of a different genetic background of the San with respect to Bantu-related populations, and highlight a possible higher risk of longer drug clearance or poor level of activation of pro-drugs among the San group.
    Full-text · Article · Feb 2016 · Acta tropica
  • Source
    • "Overall, it seems that the allele shows its highest frequency in Caucasian populations (Pechandova et al., 2012), while its frequency in Asia varies from 4.0% in India (Minhas et al., 2013) to 0.0% in Japan (Nakajima et al., 2003). In Africa, the reported frequency of the allele is 0.4% in Burkina Faso (Parikh et al., 2007), 0.0% in Ghana (Rower et al., 2005; Kudzi et al., 2009), 0.0% in Tanzania (inland) (Staehli Hodel et al., 2012), 2.1% in Zanzibar (Cavaco et al., 2005Cavaco et al., , 2013). The pfmdr1 86Y allele has been shown to be associated with CQ and AQ resistance, while the 86N allele (in combination with other pfmdr1 alleles) seems to be associated with artemeter-lumefantrine tolerance and/or resistance (Sisowhat et al., 2007). "
    [Show abstract] [Hide abstract] ABSTRACT: Study of host pharmacogenetics can improve our knowledge of mechanisms of drug resistance selection and spread. This issue has recently been addressed with respect to chloroquine and amodiaquine in malaria endemic areas of West and East Africa. Here we report, from surveys performed in two different areas of Uganda, that the human CYP2C8∗3 allele, which had been reported to be strongly associated with parasite drug resistance in Zanzibar, is absent, being a marker of genetic admixture of the Zanzibari population with a Caucasoid component. Moreover, a retrospective analysis of CYP2C8∗2 and the Plasmodium falciparum drug resistant pfmdr1 86Y allele does not show any association, which may be related to the high level of drug resistance.
    Full-text · Article · Aug 2014 · Infection Genetics and Evolution
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