High prevalence of methicillin resistant Staphylococcus aureus in pigs

Utrecht University, Utrecht, Utrecht, Netherlands
Veterinary Microbiology (Impact Factor: 2.51). 07/2007; 122(3-4):366-72. DOI: 10.1016/j.vetmic.2007.01.027
Source: PubMed


Recently methicillin resistant Staphylococcus aureus (MRSA) was isolated from pigs and pig farmers in The Netherlands. In order to assess the dissemination of MRSA in the Dutch pig population, we screened 540 pigs in 9 slaughterhouses, where a representative portion of Dutch pigs (63%) was slaughtered in 2005. We found 209 (39%) of the pigs to carry MRSA in their nares. Forty-four of 54 groups of 10 consecutive pigs (81%), each group from a different farm, and all slaughterhouses were affected. All MRSA isolates belonged to 1 clonal group, showing Multi-Locus Sequence Type 398 and closely related spa types (mainly t011, t108 and t1254). Three types of the Staphylococcal Chromosome Cassette (SCCmec) were found: III (3%), IVa (39%) and V (57%). All 44 tested isolates (1 isolate per group) were resistant to tetracycline, reflecting the high and predominant use of tetracyclines in pig husbandry. Twenty-three percent of the isolates were resistant to both erythromycin and clindamycin and 36% to kanamycin, gentamicin and tobramycin but only a single isolate was resistant to co-trimoxazole and none to ciprofloxacin and several other antibiotics. The percentage of MRSA positive pigs was significantly different among slaughterhouses and among groups within slaughterhouses, indicating a high prevalence of MRSA in pigs delivered from the farms as well as cross contamination in the slaughterhouses.

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Available from: Marga G. van Santen-Verheuvel, Oct 13, 2014
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    • "We also observed that pigs from another farm, tested MRSA negative prior to transport, but tested MRSA positive after transportation on a lorry with MRSA-positive pigs (Fig. 6). These findings are in agreement with other studies (Broens et al., 2011;de Neeling et al., 2007). The most probable sources of MRSA are lorries, lairages and/or contact to other pigs, either transported together on the same lorry or in contact at slaughterhouse. "
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    ABSTRACT: Knowledge about the dynamics of methicillin-resistant Staphylococcus aureus (MRSA) in pigs lacks detail at the level of individual animal. The aim of our study was therefore to determine the colonisation status of MRSA in individual pigs from birth to slaughter in order to gain a better understanding of substantial factors involved in transmission. Two farrow-to-finish and two grow-to-finish herds were included in the study. A total of 1728 nasal swabs from 390 pigs and 592 environmental wipes were collected at 11 different time points.
    Full-text · Article · Feb 2016 · Veterinary Microbiology
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    • "Overall, samples were taken from five dairies, which were proven positive for MRSA in previous studies. Isolates could be identified as ST398 spa t011, which also could be detected in pigs, poultry and in humans with close contact to infected animals [18] [22] [23] [24]. MRSA ST398 spa t011 has also been isolated in three dairies in Southwest Germany . "

    Full-text · Dataset · Dec 2015
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    • "The frequent detection of specific clonal MRSA lineages (CC398 MRSA) among livestock, i.e. pigs, cattle, poultry, in recent years in several (European) countries is a matter of concern (de Neeling et al., 2007; Spohr et al., 2010; Richter et al., 2012). These so-called livestock-associated (LA)-MRSA are considered to be zoonotic (van Loo et al., 2007; Witte et al., 2007b; Cuny et al., 2013) and people with occupational contact to livestock, e.g. "
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    ABSTRACT: Staphylococcus aureus is an important food-borne pathogen due to the ability of enterotoxigenic strains to produce staphylococcal enterotoxins (SEs) in food. Methicillin-resistant S. aureus (MRSA) is also an important pathogen for humans, causing severe and hard to treat diseases in hospitals and in the community due to its multiresistance against antimicrobials. In particular, strains harbouring genes encoding for the Panton-Valentine leukocidin (PVL) toxin are of concern from a public health perspective as they are usually capable of causing severe skin and soft tissue infections (sSSTIs) and occasionally necrotizing pneumonia which is associated with high mortality. This is the first report on the detection of MRSA with genes encoding for PVL in wild boar meat. Among the 28 MRSA isolated from wild boar meat in the course of a national monitoring programme in Germany, seven harboured PVL-encoding genes. Six of the isolates were identical according to the results of spa-, MLST-, microarray- and PFGE-typing. They could be assigned to the epidemic MRSA clone USA300. Epidemiological investigations revealed that people handling the food were the most likely common source of contamination with these MRSA. These findings call again for suitable hygienic measures at all processing steps of the food production chain. The results of the study underline that monitoring along the food chain is essential to closely characterise the total burden of MRSA for public health.
    Full-text · Article · Jun 2014 · International Journal of Food Microbiology
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