Membrane-Grafted Hyaluronan Films: A Well-Defined Model System of Glycoconjugate Cell Coats

Universität Heidelberg, Heidelburg, Baden-Württemberg, Germany
Journal of the American Chemical Society (Impact Factor: 12.11). 06/2007; 129(17):5306-7. DOI: 10.1021/ja068768s
Source: PubMed


We report on the creation of films of end-grafted hyaluronan, based on solid-supported lipid membranes. We characterize the layer thickness, the grafting density, the mechanical properties and the permeability of these highly hydrated and up to several hundred nanometer-thick monomolecular layers by quartz crystal microbalance with dissipation monitoring, by colloidal probe triple-wavelength reflection interference contrast microscopy, and by reflectometry. The two-dimensional assemblies thus created are expected to serve as versatile platforms to study, in a well-controlled and quantitative manner, the effect of hyaluronan-binding proteins on the structure, properties, and biological function of this type of films.

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