Microsensor Arrays for Saliva Diagnostics

Department of Periodontology and Oral Biology, Boston University, Boston, Massachusetts, United States
Annals of the New York Academy of Sciences (Impact Factor: 4.38). 04/2007; 1098(1):389-400. DOI: 10.1196/annals.1384.031
Source: PubMed


Optical fiber microarrays have been used to screen saliva from patients with end-stage renal disease (ESRD) to ascertain the efficacy of dialysis. We have successfully identified markers in saliva that correlate with kidney disease. Standard assay chemistries for these markers have been converted to disposable test strips such that patients may one day be able to monitor their clinical status at home. Details of these developments are described. In addition, saliva from asthma and chronic obstructive pulmonary disease (COPD) patients is being screened for useful diagnostic markers. Our goal is to develop a multiplexed assay for these protein and nucleic acid biomarkers for diagnosing the cause and severity of pulmonary exacerbations, enabling more effective treatment to be administered. These results are reported in the second part of this article.

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Available from: Eva J Helmerhorst, Sep 17, 2014
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    • "Considering the relatively high co-existence rate for saliva proteins and their counterpart mRNAs, the salivary transcriptome derived from DNA microarray analyses may serve as a good indicator of the diversity and range of the salivary proteome, and can be used as a reference guideline for human saliva mass spectrometry proteomic profiling [12]. For example optical fiber microarrays have been used to screen saliva from patients with end-stage renal disease (ESRD) to ascertain the efficacy of dialysis, where two salivary analytes (nitrite and uric acid) were successfully identified markers in saliva that correlate with kidney disease that were elevated in predialysis patients and were shown to be reduced following dialysis [13]. "
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