Article

Effects of light on the feedback control of GA-20 oxidase gene homolog in DongJinByeo seedlings

Division of Life Science, Soonchunhyang University, Chungnam, 336-600, Korea.
Journal of Environmental Biology (Impact Factor: 0.56). 06/2006; 27(2 Suppl):367-71.
Source: PubMed

ABSTRACT

The effects of gibberellin (GA) on the expression of GA-20 oxidase gene homolog were examined in light-grown seedlings and dark-grown seedlings of DongJinByeo. The growth rates of the stems of etiolated seedlings were faster than those of green seedlings. However, upon addition of GA to these seedlings, the stem growth rates of green seedlings were faster than those of etiolated seedlings. To understand the molecular mechanism of GA gene regulation in DongJinByeo, total RNA from DongJinByeo was hybridized with cDNA of GA-20 oxidase gene homolog. Greater accumulation of transcript of GA-20 oxidase gene homolog was observed in green seedlings than in etiolated seedlings. However, upon addition of GA, higher accumulation of the gene transcript was found in etiolated seedlings than in green seedlings, indicating that expression of the transcript of GA-20 oxidase gene homolog might be inhibited by light. These results suggest that light might regulate feedback control of the transcript of GA-20 oxidase gene homolog in DongJinByeo.

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    ABSTRACT: To understand the mechanism by which light regulates a gibberellin (GA)-insensitive gene in DongJinByeo (Oryza sativa cv DongJin), both green and etiolated DongJinByeo seedlings were submerged in water and treated with GA. Total RNA from the seedlings was isolated and hybridized with cDNA of a GA-insensitive gene homolog. The amount of transcript for the GA-insensitive gene homolog was higher in green seedlings than in etiolated seedlings in the absence of GA. However upon the addition of GA, greater accumulations of the gene transcript occurred in etiolated seedlings than in green seedlings. This result indicates the possibility that the expression of the GA-insensitive gene homolog transcript may be inhibited by light in the presence of GA. Light seems to regulate multilaterally the accumulation of the transcript of the GA-insensitive gene homolog in DongJinByeo (Oryza sativa cv DongJin).
    Preview · Article · Feb 2008 · Journal of Environmental Biology