A biocompatibility study of a reinforced acrylic based hybrid denture composite resin with polyhedraligosilsesquioxane

ArticleinJournal of Oral Rehabilitation 34(5):389-95 · June 2007with38 Reads
Impact Factor: 1.68 · DOI: 10.1111/j.1365-2842.2006.01671.x · Source: PubMed
Abstract

Acrylic-based denture materials have several common weak points, such as shrinkage after curing, lack of strength and toxicity. In order to solve these problems, we adapted a hybrid system using acrylic polymer and polyhedraloligosilsesquioxane (POSS). The aim of the study was to investigate the biocompatibility of a reinforced acrylic-based hybrid denture composite resin with POSS. Specimens of a novel polymeric denture base resin, in which POSS was used to partially replace the commonly used base monomer, were fabricated. In order to examine changes in biocompatibility with time, fresh specimens, along with specimens soaked in distilled water for 24 and 72 h were fabricated. Three other types of acrylic denture base resins were used to prepare the resin specimens. Biocompatibility (as measured by a metabolic assay, an agar overlay test, and a mutagenesis assay) of the composites was tested. The metabolic and mutagenesis assays were conducted with pure culture medium as a control. In this study, the reinforced acrylic-based hybrid denture composite resin with POSS showed improved biocompatibility and lower mutagenicity than the control. Statistical examinations showed the cell metabolic activity of the novel polymeric denture base resin in the 72-h immersion case as having almost the same inclination as the control. We hope that these results might aid in the development of a reinforced acrylic-based denture resin.

    • "...eifalian, 2006; Kannan, Salacinski, Odlyha, Butler, & Seifalian, 2006; Kannan et al., 2007; Kim et al., 2007; Punshon et al., 2005; Wang et al., 2014), and ability to modulate hydrolytic biodegradation (Gu, Wu..."
      MC is used as a binder/thickener (Cellulose & Cellulose Derivatives, 1963), as a coating material for drug tablets, in controlled drug release, sometimes in combination with the water insoluble ethylcellulose (EC) (Technical Handbook, 2000), or water-soluble polymers such as chitosan (Chen, Yeh, & Chiang, 1996; Rabea, Badawy, Stevens, Smagghe, & Steurbaut, 2003; Synytsya, Grafová, Slepicka, Gedeon, & Synytsya, 2011), polyvinyl alcohol (PVA) and polyethylene glycols (Kim, Won, Lim, & Kim, 2012; Sakellariou, Rowe, & White, 1987), and in hydrogel scaffolds (Chang & Zhang, 2011; Shoichet, 2010). Polyhedral oligomeric silsesquioxanes (POSS) nanocomposites have been investigated for use in medical device and tissue engineering applications (Kannan, Salacinski, Butler, & Seifalian, 2005;), partly due to their nontoxicity and cytocompatibility (Kannan, Salacinski, Sales, Butler, & Seifalian, 2006; Kannan, Salacinski, Odlyha, Butler, & Seifalian, 2006; Kannan et al., 2007; Kim et al., 2007; Punshon et al., 2005; Wang et al., 2014), and ability to modulate hydrolytic biodegradation (Gu, Wu, & Mather, 2011; Knight, Lee, Chung, & Mather, 2009; Lee, Knight, Chung, & Mather, 2008) and thus drug release (Guo, Knight, Wu, & Mather, 2010). Polyethylene glycol (PEG), a nontoxic oligomer of polyethylene oxide (PEO), is available in many molecular weights, is water soluble, and is used in a wide range of biomedical applications due to its ability to resist protein adhesion (Hoare & Kohane, 2008; Tessmar & Gopferich, 2007).
    [Show abstract] [Hide abstract] ABSTRACT: Blends of methyl cellulose (MC) and liquid pegylated polyoctahedralsilsesquioxane (POSS-PEG) were prepared from non-gelled, aqueous solutions at room temperature (RT), which was below their gel temperatures (Tm). Lamellar, fibrillated films (pure MC) and increasingly micro-porous morphologies with increasing POSS-PEG content were formed, which had RT moduli between 1 and 5 GPa. Evidence of distinct micro-phase separated MC and POSS-PEG domains was indicated by the persistence of the MC and POSS-PEG (at 77 K) crystal structures in the X-ray diffraction data, and scanning transmission electron images. Mixing of MC and POSS-PEG in the interface region was indicated by suppression of crystallinity in the POSS-PEG, and increases/decreases in the glass transition temperatures (Tg) of POSS-PEG/MC in the blends compared with the pure components. These interface interactions may serve as cross-link sites between the micro-phase separated domains that permit incorporation of high amounts of POSS-PEG in the blends, prevent macro-phase separation and result in rubbery material properties (at high POSS-PEG content). Above Tg/Tm of POSS-PEG, the moduli of the blends increase with MC content as expected. However, below Tg/Tm of POSS-PEG, the moduli are greater for blends with high POSS-PEG content, suggesting that it behaves like semi-crystalline polyethylene oxide reinforced with silica (SiO1.5).
    Full-text · Article · Jan 2016 · Carbohydrate Polymers
    0Comments 1Citation
    • "...nd commercial availability, POSS has been shown to offer high potential in practical applications505152535455 . However, to the best of our knowledge , amphiphilic polymer containing monofunctional POS..."
      POSScontaining amphiphilic polymers can self-assemble in aqueous solutions and form polymeric micelles which have great potential applications for drug delivery and other biological applications46474849. In addition, owing to their well-defined nanostructure, superior biocompatibility , nontoxicity, and commercial availability, POSS has been shown to offer high potential in practical applications505152535455 . However, to the best of our knowledge , amphiphilic polymer containing monofunctional POSS and thermoresponsive P(MEO 2 MA-co-OEGMA) is yet to be reported.
    [Show abstract] [Hide abstract] ABSTRACT: This article reports on the synthesis of a novel amphiphilic polyhedral oligomeric silsesquioxane (POSS) end-capped poly(2-(2-methoxyethoxy)ethyl methacrylate)-co-oligo(ethylene glycol) methacrylate) (POSS-P(MEO2MA-co-OEGMA)). These thermoresponsive organic–inorganic hybrid polymers exhibit critical phase transition temperature in water, which can be finely tuned by changing the feed ratio of OEGMA and MEO2MA. The lower critical solution temperature (LCST) of POSS-P(MEO2MA-co-OEGMA) increases from 31 to 59 °C with the increasing of OEGMA content. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) studies show that these polymers can self-assemble into spherical micelles with the thermosensitive block into the corona and the POSS forming the core, and larger aggregates are formed when the temperature values are above their LCSTs. These thermoresponsive polymers POSS-P(MEO2MA-co-OEGMA) with self-assembly behavior and tunable tempetature-responsive property have the potential applications in material science and biotechnology.
    Full-text · Article · Nov 2014 · Colloid and Polymer Science
    Shasha Li Shasha Li Yong Liu Yong Liu Sha Ji Sha Ji +1 more author... Zheng Zhou Zheng Zhou
    0Comments 1Citation
    • "...e used as fillers with polymers developed for biomedical applications, e.g., dental composites [14,86], drug delivery systems [20] and tissue engineering [98,99]. The biocompatibility of POSS because of..."
      POSS particles possess unique structures and superior properties that allow them to be used as fillers with polymers developed for biomedical applications, e.g., dental composites [14,86], drug delivery systems [20] and tissue engineering [98,99]. The biocompatibility of POSS because of their inert nature, as well as reduced inflammatory response, makes POSS nanoparticles very important in nanomedicine [14].
    [Show abstract] [Hide abstract] ABSTRACT: Hybrid materials with superior structural and functional properties can be obtained by incorporating nanofillers into polymer matrices. Polyhedral oligomeric silsesquioxane (POSS) nanoparticles have attracted much attention recently due to their nanometer size, the ease of which these particles can be incorporated into polymeric materials and the unique capability to reinforce polymers. We review here the state of POSS-containing polymer nanocomposites. We discuss the influence of the incorporation of POSS into polymer matrices via chemical cross-linking or physical blending on the structure of nanocomposites, as affected by surface functional groups, and the POSS concentration.
    Full-text · Article · Dec 2012 · Nanomaterials
    0Comments 41Citations
    • "...and bone repair [6] [7]. Other weak points of PMMA materials include lack of strength and toxicity [8]. Therefore, the search for innovative solutions addressing these problems is of special interest in..."
      Microbial adhesion has also been a limiting factor for other PMMA biomedical applications, such as ophthalmic prostheses, contact lenses and bone repair [6] [7]. Other weak points of PMMA materials include lack of strength and toxicity [8]. Therefore, the search for innovative solutions addressing these problems is of special interest in the development of acrylic materials-based implants.
    [Show abstract] [Hide abstract] ABSTRACT: Nowadays, most products for dental restoration are produced from acrylic resins based on heat-cured Poly(Methyl MethAcrylate) (PMMA). The addition of metal nanoparticles to organic materials is known to increase the surface hydrophobicity and to reduce adherence to biomolecules. This paper describes the use of nanostructured materials, TiO2 and Fe2O3, for simultaneously coloring and/or improving the antimicrobial properties of PMMA resins. Nanoparticles of metal oxides were included during suspension polymerization to produce hybrid metal oxides-alginate-containing PMMA. Metal oxide nanoparticles were characterized by dynamic light scattering, and X-ray diffraction. Physicochemical characterization of synthesized resins was assessed by a combination of spectroscopy, scanning electron microscopy, viscometry, porosity, and mechanical tests. Adherence of Candida albicans cells and cellular compatibility assays were performed to explore biocompatibility and microbial adhesion of standard and novel materials. Our results show that introduction of biocompatible metal nanoparticles is a suitable means for the improvement of conventional acrylic dental resins.
    Full-text · Article · Jan 2011 · Journal of Nanomaterials
    0Comments 18Citations
    • "...in biomedical applications, such as tissue implants (Kannan et al. 2007), dental materials (Kim et al. 2007; Siang Soh et al. 2006), and drug delivery materials (McCusker et al. 2005). The incorporation of PO..."
      On the other hand, Pan et al. (2006) have summarized the recent progress in hybrid electroluminescent materials and hybrid liquid crystals based on POSS as well as the self-assembly behavior of POSS derivatives. Polymeric POSS have also shown significant promise for use in biomedical applications, such as tissue implants (Kannan et al. 2007), dental materials (Kim et al. 2007; Siang Soh et al. 2006), and drug delivery materials (McCusker et al. 2005). The incorporation of POSS derivatives into polymeric materials can lead to dramatic improvements in polymer properties which include, but are not limited to, increase in use temperature, oxidation resistance, surface hardening, and improved mechanical properties as well as reduction in flammability, heat evolution, and viscosity during processing.
    [Show abstract] [Hide abstract] ABSTRACT: Polyhedral oligomeric silsesquioxane (POSS) are hybrid nanostructures of about 1.5nm in size. These silicon (Si)-based polyhedral nanostructures are attached to a polystyrene (PS) backbone to produce a polymer nanocomposite (POSS–styrene). We have solution blended POSS–styrene of [`(M)]w = 14.5103g/mol\overline{M}_w =14.5\times 10^3\;\rm{g/mol} with commercial polystyrene (PS), [`(M)]w = 2.8105g/mol\overline{M}_w =2.8\times 10^5\;\rm{g/mol}, and studied the rheological behavior and thermal properties of the neat polymeric components and their blends. The concentration of POSS–styrene was varied from 3 up to 20wt.%. Thermal analysis studies suggest phase miscibility between POSS–styrene and the PS matrix. The blends displayed linear viscoelastic regime and the time–temperature superposition principle applied to all blends. The flow activation energy of the blends decreased gradually with respect to the matrix as the POSS–styrene concentration increased. Strikingly, it was found that POSS–styrene promoted a monotonic decrease of zero-shear rate viscosity, η 0, as the concentration increased. Rheological data analyses showed that the POSS–styrene increased the fractional free volume and decreased the entanglement molecular weight in the blends. In contrast, blending the commercial PS with a PS of [`(M)]w = 5103g/mol\overline{M}_w =5\times 10^3\;\rm{g/mol} did not show the same lubrication effect as POSS–styrene. Therefore, it is suggested that POSS particles are responsible for the monotonic reduction of zero-shear rate viscosity in the blends.
    Full-text · Article · Jul 2009 · Rheologica Acta
    0Comments 20Citations
    • "...pal inflammatory response. In the pulp, various cell types, such as odontoblasts [28], fibroblasts [29] and cells involved in the immune response, i.e. monocytes [30] could respond to these materials. Mo..."
      Components from resin materials released into the moist environment, such as dentine, could diffuse into dentinal tubules and reach the pulp tissue, eliciting a pulpal inflammatory response. In the pulp, various cell types, such as odontoblasts [28], fibroblasts [29] and cells involved in the immune response, i.e. monocytes [30] could respond to these materials. Monocytes/ macrophages are the main cells which immediately react to foreign materials.
    [Show abstract] [Hide abstract] ABSTRACT: The antimicrobial effect and biocompatibility of insoluble cross-linked quaternary ammonium polyethylenimine (PEI) nanoparticles incorporated at 1 or 2%w/w in a resin composite were assayed. The antimicrobial effect against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli was tested using the direct contact test (DCT), agar diffusion test (ADT) and scanning electron microscopy (SEM). Biocompatibility was tested by assessing macrophage viability, and TNFalpha secretion. Samples incorporating 2%w/w nanoparticles inhibited the growth of all bacterial strains tested. Reducing the amount of the added nanoparticles to 1%w/w resulted in complete inhibition of S. aureus and E. faecalis, and decreased growth of S. epidermidis, P. aeruginosa and E. coli (p<0.0001). The DCT results were confirmed by SEM. However, ADT showed no inhibition halo in all test bacteria, indicating the antimicrobial nanoparticles are not diffusing into the agar milieu. Biocompatibility tests revealed macrophage viability, and TNFalpha secretion was not altered by the presence of the nanoparticles in the resin. Incorporation of PEI nanoparticles in a resin composite had a long lasing antimicrobial effect against a wide range of bacteria with no measured effect on biocompatibility.
    Full-text · Article · Nov 2008 · Biomaterials
    0Comments 86Citations
Show more