Free Radical Scavenging Activity of a Novel Antioxidative Peptide Isolated from In Vitro Gastrointestinal Digests of Mytilus coruscus

Department of Chemistry, Pukyong National University, Busan, Republic of Korea.
Journal of Medicinal Food (Impact Factor: 1.63). 04/2007; 10(1):197-202. DOI: 10.1089/jmf.2006.101
Source: PubMed


A low-molecular-weight peptide with potent antioxidative activity was obtained from Mytilus coruscus muscle protein using an in vitro gastrointestinal digestion system. The potent antioxidant peptide, which was identified as Leu-Val-Gly-Asp-Glu-Gln-Ala-Val-Pro-Ala-Val-Cys-Val-Pro (1.59 kDa), exhibited higher protective activity against polyunsaturated fatty acid (PUFA) peroxidation than the native antioxidants, ascorbic acid and alpha-tocopherol. In a free radical scavenging assay using electron spin resonance spectroscopy, hydroxyl radical formation was quenched by 75.04% in the presence of M. coruscus peptide (50 microg/mL), which was similar to ascorbic acid. In addition, the purified peptide could also quench super-oxide and carbon-centered radicals, but those activities were weaker than for ascorbic acid. This study showed that the low-molecular-weight peptide released from in vitro gastrointestinal digestion of mussel exhibited potent antioxidant potential by inhibiting the formation of reactive oxygen species formed by the peroxidation of PUFAs.

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    • "These methods have been used to evaluate the antioxidant effects of anthocyanins [14] [15], phenolics [16] and flavanones [17]. Some food proteins and protein hydrolysates have been reported to have antioxidant capacity [18] [19] [20]. Proteins in raw and processed food can possess antioxidant peptide sequences and structural domains and the active fragments are released during the GI digestion process [21]. "

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    • "With the advantage that the formed peptides will resist physiological digestion after oral intake, the digestion by gastrointestinal proteases can be useful as a production process for antioxidant peptides. Some recent studies have reported that in vitro gastrointestinal digests of marine organisms possessed biological activities as potent as that of other natural antioxidants (Jung et al., 2007; Qian et al., 2008). Abalone is a marine gastropod, and one of the most commercially important fishery resources in Asian cultures (Zhu et al., 2008). "
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    ABSTRACT: Abalone (Haliotis discus hannai), mostly distributed and maricultured in southwestern coastal areas of South Korea, is recognized as an economically important species in the fishery industry. Abalone intestines are one of the by-products of abalone processing. To investigate abalone intestines as bioactive substances, abalone intestine gastrointestinal digests (AIGIDs) of various molecular weights (MWs) were prepared using in vitro gastrointestinal digestion and an ultrafiltration system, and tested for inhibitory effects against reactive oxygen species (ROS) and oxidative stress in macrophage cells treated with hydrogen peroxide (). In our results, among AIGIDs, AIGID-III (MW=5-10 kDa) showed potent inhibitory activities for lipid peroxidation and free radicals. Additionally, the results clearly indicated that AIGID-III treatment could prevent cytotoxic damage of macrophages by -induced oxidative stress due to its potent scavenging ability against cellular ROS. These results suggest that AIGIDs may have protective and therapeutic potential for oxidative stress syndromes and immune diseases through ROS inhibition in macrophage cells.
    Full-text · Article · Sep 2010 · Fisheries and Aquatic Science
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    • "Proteins in raw and processed foods can possess antioxidant peptide sequences and structural domains; the active fragments are released during the GI digestion process. Reported highefficiency radical scavenging peptides released through in vitro pepsin and pancreatin digestion include those from casein (Hernandez-Ledesma, Amigo, Ramos, & Recio, 2004), maize zein (Zhu, Chen, Tang, & Xiong, 2008), oyster protein (Crassostrea gigas) (Qian, Jung, Byun, & Kim, 2008), and mussel protein (Mytilus coruscus) (Jung et al., 2007). "
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    ABSTRACT: Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2 h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2'-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS(+•)) and hydroxyl ((•)OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS(+•) scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralizing (•)OH and was 86 and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominant peptides/amino acid in these fractions.
    Full-text · Article · Feb 2010 · Food Chemistry
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