Mixing in 384-Well Plates: Issues, Measurements, and Solutions

ArticleinAssay and Drug Development Technologies 5(2):265-75 · May 2007with79 Reads
Impact Factor: 1.53 · DOI: 10.1089/adt.2006.042 · Source: PubMed


    Mixing in standard 384-well plates is different from mixing in 96-well formats. The aspect ratio of a typical well, the balance of surface tension and mass of the fluids, and the scale of diffusion all add to the increased difficulty in mixing fluids in higher-density plates. Here we examine two methods to measure mixing and some common techniques for mixing in 384-well plates. While conventional shaking can suffice, alternative methods can accelerate and improve the efficiency of mixing in 384-well plates.