Thirteen-lined ground squirrels (Spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria

Department of Plant Pathology, University of Wisconsin–Madison, Madison, Wisconsin, United States
Journal of the American Association for Laboratory Animal Science: JAALAS (Impact Factor: 1.12). 06/2007; 46(3):21-3.
Source: PubMed


Whether wild-caught animals used for biomedical research carry antibiotic-resistant bacteria is not well studied. Thirteen- lined ground squirrels (Spermophilus tridecemlineatus) are small mammals used to study hibernation. These animals are captured from the wild or are born in laboratory animal facilities to wild-caught mothers. Because microorganisms harbored by 13-lined ground squirrels may be pathogenic to their caretakers and other laboratory animals, learning more about antibiotic resistance in these animals could be useful. In this study, tetracycline- and chloramphenicol-resistant Morganella morganii and multidrug resistant Stenotrophomonas maltophilia were isolated from the ceca of four 13-lined ground squirrels. These findings support further study of antibiotic-resistant bacterial populations in wild-caught mammals used as laboratory models.

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    • "Although enzyme activity is relatively slow at the low temperatures of deep torpor (o10 1C), host-derived substrates can be rapidly degraded during interbout arousals and result in the production of SCFAs and other microbial metabolites (Carey et al., 2013; Duddleston et al., 2012; Stevenson, 2014). Previous culture-based studies in thirteen-lined ground squirrels indicated that microbes associated with cecal contents ( " luminal microbiota " ) were reduced in number and species composition during hibernation compared to summer active ( " summer " ) squirrels (Barnes and Burton, 1970), and bacteria could be readily cultured from mucosal tissue of hibernators (Cloud-Hansen et al., 2007). However, as noted previously, culture-based studies are limited because the majority of gut microbes are unculturable with current techniques. "
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    Full-text · Article · Aug 2014 · Journal of Thermal Biology
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    • "There is limited information relating to S. maltophilia infection in animals. Although isolated from fish and snakes (Hejnar et al., 2007), from bovine and ovine milk (Litopoulou-Tzanetaki and Vafopoulou-Mastrojiannaki, 1995), from rabbit faeces (Draper et al., 1981), and from the gastrointestinal tract of laboratory animals (Toledo-Pereyra et al., 1974; Cloud-Hansen et al., 2007), S. maltophilia has only once been reported as an equine pathogen (Albini et al., 2009). However, one in vitro study of S. maltophilia and influenza concluded that co-infection with S. maltophilia could enhance the pathogenicity of equine influenza virus (Mancini et al., 2005). "
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    ABSTRACT: Only little is known about normal oral bacterial flora in captive snakes containing Stenotrophomonas maltophilia. This microbe has been reported as a causative agent of numerous infections in reptiles. Therefore, the goal of the study was to detect its presence in the mouths of a significant number of healthy captive snakes and determining its susceptibility to antibiotics at 30 and 37 degrees C. The isolates were obtained in 1999-2005 from mouth swabs of 115 snakes of 12 genera and 22 species-most often Elaphe guttata (24 individuals; 20.9%). Susceptibility to 24 antibiotics was tested by the microdilution method. The microbe was demonstrated in 34 (29.6%) individuals. Overall, 47 strains of S. maltophilia were acquired. Evaluation using PFGE profiles and antibiograms resulted in confirmation of one strain of S. maltophilia in 23 (20.0%) individuals, two strains in nine (7.8%) and three in two (1.8%) snakes. All tested antibiotics were more effective at 37 degrees C, with the partial exception of cotrimoxazole and cefoperazone/sulbactam. At a temperature of 37 degrees C, the lowest frequency of resistance to levofloxacin (no resistant strains), cotrimoxazole and ofloxacin (97.9% of susceptible strains) was recorded. At 30 degrees C, the most active agents were cotrimoxazole (97.9% of susceptible strains), levofloxacin (91.5%) and ofloxacin (85.1%). In conclusion, S. maltophilia is present in the mouths of about one third of healthy captive snakes, showing good susceptibility to cotrimoxazole, some fluoroquinolones and aminoglycosides. The antibiotics (particularly aminoglycosides) are more effective at 37 degrees C.
    Full-text · Article · May 2007 · Veterinary Microbiology
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    ABSTRACT: Stenotrophomonas maltophilia (S. maltophilia) is a nonfermentative bacterium, which is naturally resistant against a panel of commonly-used antibiotics. It is frequently isolated from humans with chronic respiratory disease, e.g. cystic fibrosis or chronic obstructive pulmonary disease. In veterinary medicine S. maltophilia is perceived to be a mere coloniser. We herewith report 7 strains of S. maltophilia isolated from animals, of which 5 strains were harvested from 3 horses, a dog and a cat with chronic respiratory disease. The dog isolate showed resistance to trimethoprim / sulphamethoxazole, which was confirmed by detection of the sul 1 gene. Analysis with pulsed field gel electrophoresis revealed that 2 horses, which were boarded in the same clinic but two years apart, harboured the same strain of S. maltophilia. This is indicative of a hospital acquired colonisation / infection, which contradicts involvement in the pre-existing chronic disease.
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