Article

The generation of adipocytes by the neural crest

Institut de Recherche, Signalisation, Biologie du Développement et Cancer, CNRS UMR 6543, Centre de Biochimie, Faculté des Sciences, Université Nice Sophia-Antipolis, Nice, France.
Development (Impact Factor: 6.46). 07/2007; 134(12):2283-92. DOI: 10.1242/dev.002642
Source: PubMed

ABSTRACT

Fat cells (adipocytes) develop from adipocyte precursor cells (preadipocytes) that themselves derive from mesenchymal progenitors. Although the events controlling preadipocyte differentiation into mature adipocytes have been largely explored, the mechanisms that direct mesenchymal progenitors down the adipocyte pathway remain unknown. Similarly, although adipocytes are generally thought to derive from mesoderm, key information is lacking regarding the origin and the development of the adipose tissue during embryogenesis. The aim of this study was to gain insight into the ontogeny of fat cells, both in mouse embryonic stem (mES) cell-derived cultures and during normal development. We first used genetically engineered mES cells to produce and select ES cell-derived neuroepithelial progenitors and showed that neuroectoderm, rather than mesoderm, may be a source of adipocytes in mES cell-derived cultures. We then used primary and secondary cultures of developing quail neural crest (NC) cells to demonstrate that NC cells are able, upon stimulation with defined factors, to differentiate into adipocytes, thus providing a powerful system to study the earliest stages of adipocyte differentiation. Finally, we mapped NC derivatives in vivo using Cre-mediated recombination in transgenic mice and demonstrated that a subset of adipocytes originates from the NC during normal development.

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    • "However, while NCDASC maintain their predilection towards an adipogenic lineage, they show reduced osteogenic and chondrogenic potential[75]. Another group also confirmed that a subset of adipocytes originate from the neural crest; as well, the neural-crest derived subpopulation under the proper stimulation will differentiate into adipocytes[77]. Taken together, neck-derived ASC and NCDASC show multipotentiality, but reduced osteogenic and chondrogenic potential compared to ASC derived from other sites. "

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    • "These results are in accordance with previous reports [42], [43]. In our study, both cNCCs and tNCCs differentiated into adipocytes, as shown in a previous study [44], although trunk EGFP− cells produced the highest number of adipocytes. This result is quite reasonable, because trunk EGFP− cells include a relatively large proportion of mesodermal stem cells that can differentiate into osteocytes, chondrocytes and adipocytes. "
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    ABSTRACT: The outstanding differentiation capacities and easier access from adult tissues, cells derived from neural crest cells (NCCs) have fascinated scientists in developmental biology and regenerative medicine. Differentiation potentials of NCCs are known to depend on their originating regions. Here, we report differential molecular features between craniofacial (cNCCs) and trunk (tNCCs) NCCs by analyzing transcription profiles and sphere forming assays of NCCs from P0-Cre/floxed-EGFP mouse embryos. We identified up-regulation of genes linked to carcinogenesis in cNCCs that were not previously reported to be related to NCCs, which was considered to be, an interesting feature in regard with carcinogenic potentials of NCCs such as melanoma and neuroblastoma. Wnt signal related genes were statistically up-regulated in cNCCs, also suggesting potential involvement of cNCCs in carcinogenesis. We also noticed intense expression of mesenchymal and neuronal markers in cNCCs and tNCCs, respectively. Consistent results were obtained from in vitro sphere-forming and differentiation assays. These results were in accordance with previous notion about differential potentials of cNCCs and tNCCs. We thus propose that sorting NCCs from P0-Cre/floxed-EGFP mice might be useful for the basic and translational research of NCCs. Furthermore, these newly-identified genes up-regulated in cNCC would provide helpful information on NC-originating tumors, developmental disorders in NCC derivatives, and potential applications of NCCs in regenerative medicine.
    Full-text · Article · Jan 2014 · PLoS ONE
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    • "We identified only a few NC-derived GFP+ adipocytes in the head and almost none in the trunk despite their efficient adipogenic potential in vitro. This is consistent with in vivo fate mapping of either Sox10-Cre or Wnt1-Cre lineage analysis [13], [41]. One possible explanation is that NCDASCs colonize earlier but are largely replaced by non-NC derivatives before in vivo adipogenesis occurs. "
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    ABSTRACT: Recent studies have shown that adipose-derived stromal/stem cells (ASCs) contain phenotypically and functionally heterogeneous subpopulations of cells, but their developmental origin and their relative differentiation potential remain elusive. In the present study, we aimed at investigating how and to what extent the neural crest contributes to ASCs using Cre-loxP-mediated fate mapping. ASCs harvested from subcutaneous fat depots of either adult P0-Cre/or Wnt1-Cre/Floxed-reporter mice contained a few neural crest-derived ASCs (NCDASCs). This subpopulation of cells was successfully expanded in vitro under standard culture conditions and their growth rate was comparable to non-neural crest derivatives. Although NCDASCs were positive for several mesenchymal stem cell markers as non-neural crest derivatives, they exhibited a unique bipolar or multipolar morphology with higher expression of markers for both neural crest progenitors (p75NTR, Nestin, and Sox2) and preadipocytes (CD24, CD34, S100, Pref-1, GATA2, and C/EBP-delta). NCDASCs were able to differentiate into adipocytes with high efficiency but their osteogenic and chondrogenic potential was markedly attenuated, indicating their commitment to adipogenesis. In vivo, a very small proportion of adipocytes were originated from the neural crest. In addition, p75NTR-positive neural crest-derived cells were identified along the vessels within the subcutaneous adipose tissue, but they were negative for mural and endothelial markers. These results demonstrate that ASCs contain neural crest-derived adipocyte-restricted progenitors whose phenotype is distinct from that of non-neural crest derivatives.
    Full-text · Article · Dec 2013 · PLoS ONE
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