Microfluidic devices for size-dependent separation of liver cells

Department of Chemical Engineering, Osaka Prefecture University, Sakai, Ōsaka, Japan
Biomedical Microdevices (Impact Factor: 2.88). 11/2007; 9(5):637-45. DOI: 10.1007/s10544-007-9055-5
Source: PubMed


Liver is composed of various kinds of cells, including hepatic parenchymal cells (hepatocytes) and nonparenchymal cells, and separation of these cells is essential for cellular therapies and pharmacological and metabolic studies. Here, we present microfluidic devices for purely hydrodynamic and size-dependent separation of liver cells, which utilize hydrodynamic filtration. By continuously introducing cell suspension into a microchannel with multiple side-branch channels, cells smaller than a specific size are removed from the mainstream, while large cells are focused onto a sidewall in the microchannel and then separated into two or three groups. Two types of PDMS-glass hybrid microdevices were fabricated, and rat liver cells were successfully separated. Also, cell size, morphology, viability and several cell functions were analyzed, and the separation performances of the microfluidic devices were compared to that of a conventional centrifugal technique. The results showed that the presented microfluidic devices are low-cost and suitable for clinical use, and capable of highly functional separation with relatively high-speed processing.

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    • "Efforts to successfully separate these specific cells from the blood have resulted in the development of lab-on-chip technologies. Among the various ways to separate the target cells, the method of cell transportation by microfluidic systems has several advantages (Stott et al. 2010; Park and Jung 2009; Bhagat et al. 2011; Hou et al. 2010; Yamada et al. 2007; Gossett et al. 2010; McFaul et al. 2012; Tanaka et al. 2012), including low cost, small sample and reagent requirements, and device portability (Tsutsui and Ho 2009). However, because of the complex microfluidics of cell movement inside channels, high-yield or high-selectivity fluidic conditions for cell separation systems have not yet been achieved. "
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    ABSTRACT: We investigate the path selection (navigation) of a single moving vesicle in a microfluidic channel network using a lattice Boltzmann-immersed boundary method (IBM). The lattice Boltzmann method is used to determine incompressible fluid flow with a regular Eulerian grid. The IBM is used to study a vesicle with a Lagrangian grid. Previous studies of microchannels suggest that the path selection of a bubble at a T-shaped junction depends on the flow rates in downstream channels. We perform simulations to observe the path selection of a vesicle with three different capillary numbers at a tertiary junction. The hypothesis that higher flow rate determines path selection is not validated by our data on low capillary number (Ca ≤ 0.025) of a vesicle in tertiary downstream channels. We use the resultant velocity hypothesis to explain the path selection of a vesicle in microfluidic systems. Our results suggest that, for a low capillary number, instead of being affected by the viscous force from a high flow rate, a vesicle in a tertiary junction tends to follow the resultant velocity hypothesis. We analyze the change in hydrodynamic resistance caused by the movement of a vesicle to support the resultant velocity hypothesis. We also study the residence time of a vesicle at a junction for different cases and analyze the relationship between the residence time and the resultant velocity. The resultant velocity (rather than the flow rate in individual channels) can be used to predict the path selection of a vesicle in low capillary number. In addition, the residence time of vesicle is decided by average velocity of each channel.
    Full-text · Article · Dec 2014 · Microfluidics and Nanofluidics
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    • "In each trap, single microbeads are trapped based on the dynamic change in the hydraulic resistance (i.e., before and after microbead trapping) as a function of the cross-sectional shape of a microchannel. Briefly, depending on the relationship between the center position of a microbead and the flow pattern [i.e., a virtual width (W v )], the path of the introduced microbeads is determined (Yamada et al. 2007). First, to regulate the position of microbeads and to ensure the trapping of single microbeads, all introduced microbeads are aligned along one side wall using the buffer flow. "
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    ABSTRACT: A simple yet effective dynamic bead-based microarray is necessary for multiplexed high-throughput screening applications in the fields of biology and chemistry. This paper introduces a microfluidic-based dynamic microbead array system using pneumatically driven elastomeric valves integrated with a microchannel in a single polydimethylsiloxane (PDMS) layer that performs the following functions: single-microbead arraying with loading and trapping efficiencies of 100 %, sequential microbead release for selective retrieval of microbeads of interest, and rapid microarray resettability ( Keywords: Device resettability; Microfluidics; Sequential release; Single-layer pneumatic valve; Single-microbead array Document Type: Research Article DOI: Affiliations: 1: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Pohang, Kyungbuk, 790-784, Republic of Korea 2: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Pohang, Kyungbuk, 790-784, Republic of Korea, Email: Publication date: April 1, 2014 $(document).ready(function() { var shortdescription = $(".originaldescription").text().replace(/\\&/g, '&').replace(/\\, '<').replace(/\\>/g, '>').replace(/\\t/g, ' ').replace(/\\n/g, ''); if (shortdescription.length > 350){ shortdescription = "" + shortdescription.substring(0,250) + "... more"; } $(".descriptionitem").prepend(shortdescription); $(".shortdescription a").click(function() { $(".shortdescription").hide(); $(".originaldescription").slideDown(); return false; }); }); Related content In this: publication By this: publisher By this author: Kim, Hojin ; Kim, Joonwon GA_googleFillSlot("Horizontal_banner_bottom");
    Full-text · Article · Apr 2014 · Microfluidics and Nanofluidics
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    • "For these types of sensors, size-dependent particle separation would allow different-sized micro/nano particles to be guided to different sensing channels with corresponding sizes, thus greatly improving the sensitivity and dynamic range at a high throughput. To date various techniques for particle or cell separation have been studied including hydrodynamic filtration [6,7], deterministic lateral displacement [8,9], pinched flow fractionation [10,11], sedimentation [12], inertial [13,14], magnetophoresis [15,16], negative magnetophoresis [17,18], optical [19,20] and dielectrophoresis [21,22]. "
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    ABSTRACT: Particle separation is of great interest in many biological and biomedical applications. Flow-based methods have been used to sort particles and cells. However, the main challenge with flow based particle separation systems is the need for a sheath flow for successful operation. Existence of the sheath liquid dilutes the analyte, necessitates precise flow control between sample and sheath flow, requires a complicated design to create sheath flow and separation efficiency depends on the sheath liquid composition. In this paper, we present a microfluidic platform for sheathless particle separation using standing surface acoustic waves. In this platform, particles are first lined up at the center of the channel without introducing any external sheath flow. The particles are then entered into the second stage where particles are driven towards the off-center pressure nodes for size based separation. The larger particles are exposed to more lateral displacement in the channel due to the acoustic force differences. Consequently, different-size particles are separated into multiple collection outlets. The prominent feature of the present microfluidic platform is that the device does not require the use of the sheath flow for positioning and aligning of particles. Instead, the sheathless flow focusing and separation are integrated within a single microfluidic device and accomplished simultaneously. In this paper, we demonstrated two different particle size-resolution separations; (1) 3 μm and 10 μm and (2) 3 μm and 5 μm. Also, the effects of the input power, the flow rate, and particle concentration on the separation efficiency were investigated. These technologies have potential to impact broadly various areas including the essential microfluidic components for lab-on-a-chip system and integrated biological and biomedical applications.
    Full-text · Article · Dec 2012 · Sensors
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