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Temporal progression and extent of the return of sensation in the foot provided by the saphenous nerve after sciatic nerve transection and repair in the rat - Implications for nociceptive assessments

Chair of General Pathology & Neuropathology, Institute of Veterinary Pathology, Ludwig-Maximilians University, Munich, Germany.
Somatosensory and Motor Research (Impact Factor: 0.64). 03/2007; 24(1-2):1-13. DOI: 10.1080/08990220601116329
Source: PubMed

ABSTRACT

Sensory testing, by providing stimuli for nociceptors of the foot, is a popular method of evaluating sensory regeneration after damage to the sciatic nerve in the rat. In the following study, 20 rats were submitted to double transection of the sciatic nerve. The subsequent 14 mm gap was repaired through guidance interponation. In order to evaluate nerve regeneration, sensory testing was performed additionally to other methods, which included motor testing, morphometry, and electron microscopic assessments of nerves. Somatosensory testing revealed that all animals exhibited next to the same amount of sensory reinnervation on their foot regardless of their experimental group. In motor tests, however, two out of the three experimental groups did not improve at all. These groups also failed to show neural regrowth in morphometric and electron microscopic assessments of the associated nerve. Retrograde tracing was able to prove the saphenous nerve as an alternative source of sensory reinnervation in animals with failed sciatic regeneration. This means that results of sensory testing in the rat should be treated with caution, taking into account the areas tested and the likelihood that in these areas saphenous sprouting could have taken place. Furthermore, it is strongly advised that somatosensory testing should be conducted only on toe 5.

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    • "Such motor dependency can be circumvented if the response to the stimulus can be elicited from an area of the body which was unaffected by the nerve injury (Nichols et al., 2005) Animals display a hypersensitive withdrawal response following nerve injuries that is associated with sensitization of cutaneous nociceptors to mechanical and thermal stimuli. An important consideration for the validity of these behavioural tests is to ensure that evaluation is done in areas of the foot that are definitely not affected by sprouting of neighbouring intact nerves, taking into account the territories of innervation of the sciatic and saphenous nerves in the hindpaw (Fig. 4C), and of median and ulnar nerves in the forepaw (Rupp et al., 2007; Cobianchi et al., 2014). On the other hand, selective stimulation applied to specific sites in the paw allow assessment in parallel of nerve regeneration and collateral sprouting effects on pain and reinnervation. "
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    ABSTRACT: Peripheral nerve injuries usually lead to severe loss of motor, sensory and autonomic functions in the patients. Due to the complex requirements for adequate axonal regeneration, functional recovery is often poorly achieved. Experimental models are useful to investigate the mechanisms related to axonal regeneration and tissue reinnervation, and to test new therapeutical strategies to improve functional recovery. Therefore, objective and reliable evaluation methods should be applied for the assessment of regeneration and function restitution after nerve injury in animal models. This review gives an overview of the most useful methods to assess nerve regeneration, target reinnervation and recovery of complex sensory and motor functions, their values and limitations. The selection of methods has to be adequate to the main objective of the research study, either enhancement of axonal regeneration, improving regeneration and reinnervation of target organs by different types of nerve fibers, or increasing recovery of complex sensory and motor functions. It is generally recommended to use more than one functional method for each purpose, and to perform also morphological studies of the injured nerve and the reinnervated targets. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    No preview · Article · Jul 2015 · European Journal of Neuroscience
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    • "The surgical approach for exposing the sciatic nerve, the length and location of the segment excised from the sciatic nerve, the type and location of closing-up sutures, anaesthesia and perioperative management were identical to those applied in the previous study (Rupp et al., 2007b). "
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    ABSTRACT: Striking inconsistencies between the results of morphometric and electrophysiologic examinations of the regenerating nerve were observed in a previous study featuring the bridging of a 14 mm gap in the rat sciatic nerve. To shed light on this dichotomy, seven further rats were subjected to permanent sciatic nerve transection and assessed electrophysiologically, histologically and by retrograde axonal tracing at various postoperative intervals (1 h to 8 weeks). The results of the histological examinations and retrograde tracing revealed that in spite of the fact that compound muscle action potentials could be recorded in the gastrocnemius muscle, no reinnervation of the gastrocnemius muscle, either physiological or aberrant, had actually taken place. Furthermore, it was established that the electrical activity recorded in the gastrocnemius muscle after stimulation of the proximal or distal stump is generated by surrounding hind limb muscles unaffected by denervation. These are stimulated either directly, or indirectly due to spreading of the impulse. It is therefore strongly recommended that caution should be exercised when interpreting recordings from the gastrocnemius muscle after stimulation of a regenerating sciatic nerve in laboratory rodents.
    Full-text · Article · Dec 2007 · Journal of Neuroscience Methods
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