Molecular Cloning and the Allergenic Characterization of Tropomyosin from Tyrophagus putrescentiae

ArticleinProtein and Peptide Letters 14(5):431-6 · February 2007with11 Reads
DOI: 10.2174/092986607780782777 · Source: PubMed
Abstract
Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.
    • "It can vector pathogens, such as Aspergillus flavus Link and Fusarium poae (Peck) Wollenw to sterile grain and mushroom beds, and can also promote nonbeneficial fungal growth, increasing aflatoxin and deoxynivalenol production (Franzolin et al. 1999, Hubert et al. 2013b). T. putrescentiae infests the mycelia and fruiting bodies of edible mushrooms, decreasing production and causing mildews on dried mushrooms, human allergic dermatitis , pulmonary acariasis, and intestinal acariasis (Li et al. 2003, Jeong et al. 2007 ). The mite seriously affects both the economic efficiency of mushroom production and the health of mushroom growers (Zhang et al. 1992). "
    [Show abstract] [Hide abstract] ABSTRACT: China is the largest producer, consumer, and exporter of mushrooms in the world. The storage mite, Tyrophagus putrescentiae Schrank, is one of the most important arthropod pests in mushroom cultivation. This study investigated the development and reproductive traits of this mite reared on four mushroom species: Agaricus bisporus Lange, Pleurotus ostreatus Kumm, Auricularia polytricha (Mont.) Sacc., and Flammulina velutipes (Fr.) Sing., at seven constant temperatures ranging from 16 to 34°C at 80% relative humidity. Development time for the immature stages decreased with increasing temperature, and was also significantly affected by mushroom species. The shortest immature developmental period (7.0 ± 0.2 d) was observed at 31°C when reared on F. velutipes, while the longest development was at 16°C (36.0 ± 0.3 d) reared on P. ostreatus. The effects of temperature and mushroom hosts on the development, female longevity, and reproduction were also significant. The lower threshold temperatures from egg-to-adult for the four mushroom species were 11.97, 12.02, 10.80, and 11.57°C, for A. bisporus, P. ostreatus, Au. polytricha, and F. velutipes, and the thermal constants were 133.3, 136.8, 165.2, and 135.9 degree days (°C d), for the same mushroom species, respectively. Life table parameters at 25°C were estimated as follows: net reproductive rates (R0), 59.16, 28.94, 42.62, and 62.93, and intrinsic rate of natural increase (rm), 0.24, 0.13, 0.17, and 0.24, respectively. These results suggest that these mushrooms are suitable hosts for T. putrescentiae, and the storage mite may be able to adapt to higher temperatures.
    Full-text · Article · Apr 2015
    S. X. QuS. X. QuH. P. LiH. P. LiL. MaL. Ma+1more author...[...]
    • "According to statistics, its mushroom hosts include Lentinula edodes (Berk), Agaricus bisporus (Lange), Pleurotus ostreatus (Kumm), Ganoderma sp., and Pleurotus pulmonarius (Fr.). It can carry and spread pathogens, reduce mushroom production, cause dried mushroom mildew, and cause human allergic dermatitis , pulmonary acariasis, and intestinal acariasis (Li et al., 2003; Jeong et al., 2007), seriously affecting the economic efficiency of mushroom production and the health of mushroom farmers. According to the Ministry of Agriculture, Chinese edible mushroom output has reached more than 10 million tons annually in recent years, accounting for over 70% of the total global production. "
    [Show abstract] [Hide abstract] ABSTRACT: The storage mite, Tyrophagus putrescentiae (Schrank), infests various stored products, plant seeds, chinese herbal medicines and edible mushrooms in China. To assess interactions between edible mushroom hosts and the mite, we investigated the effects of nine species on development and reproduction at 22 °Cand 85% humidity in the laboratory. We also demonstrated changes in the bacterial community of T. putrescentiae reared on four mushroom hosts. All the immature stages, female longevity and reproductive periods were significantly affected by the mushroom species, total immature developmental time varied from 4.41 ± 0.20 (reared on Auricularia polytricha) to 9.79 ± 0.92 days (when reared on Ganoderma lucidum). The egg stage was the longest period of the immature stages, whereas the protonymph and tritonymph stages were the shortest at about 2 days. The net reproductive rate (R0), and the intrinsic rate of increase (rm) were significantly different on the nine mushroom hosts. Both the maximum fecundity (212.85 eggs per female) and the highest rm were obtained on A. polytricha. The results indicated that T. putrescentiae had a wide range of adaptability to edible mushrooms, with development and reproduction of the mite being significantly affected by different species. Changes in the bacterial communities inhabiting the mites were influenced by mushroom hosts. A total of 30 full length sequences of 16s rRNA gene were obtained from the mite reared on four hosts. The bacterial community inhabiting T. putrescentiae on A. polytricha host was the most diverse with six different species, two of these genera occurred on Flammulina velutipes host. Consequently the interactions between T. putrescentiae and mushroom hosts can influence both the biological characteristics and bacterial community. The changes of bacterial community might participate in the different fungal mycelium hydrolysis.
    Full-text · Article · Dec 2014
    S.-X. QuS.-X. QuH.-P. LiH.-P. LiL. MaL. Ma+1more author...[...]
    • "The recombinant allergen was detected in 6.4% of sensitized patients [122]. Recombinant Tyr p 10 has 64%–94% shared aa identity with other allergenic tropomyosins and was recognized by 12.5% of sera from sensitized patients [123]. α-Tubulin is a putative allergen from a phage display cDNA library of T. putrescentiae. "
    [Show abstract] [Hide abstract] ABSTRACT: Allergic diseases triggered by mite allergens include allergic rhinoconjunctivitis, asthma, atopic dermatitis and other skin diseases. Since the early discovery of the allergenic role of mites of the genus Dermatophagoides in the mid 1960s, numerous species have been described as the source of allergens capable of sensitizing and inducing allergic symptoms in sensitized and genetically predisposed individuals. The main sources of allergens in house dust worldwide are the fecal pellets of the mite species D. pteronyssinus, D. farinae, Euroglyphus maynei and the storage mites Blomia tropicalis, Lepidoglyphus destructor and Tyropahgus putrescentiae. Group 1 and 2 allergens are major house dust mite allergens. The main allergens in storage mites include fatty acid-binding proteins, tropomyosin and paramyosin homologues, apolipophorin-like proteins, α-tubulins and others, such as group 2, 5 and 7 allergens. Cross-reactivity is an important and common immunological feature among mites. Currently, purified native or recombinant allergens, epitope mapping, proteomic approaches and T cell proliferation techniques are being used to assess cross-reactivity. Mites contain potent enzymes capable of degrading a wide range of substrates. Most mite allergens are enzymes. Advances in genomics and molecular biology will improve our ability to understand the genetics of specific IgE responses to mites. Mite allergen avoidance and immunotherapy are the only two allergen-specific ways to treat mite-induced respiratory and cutaneous diseases. © 2014 S. Karger AG, Basel.
    Full-text · Article · May 2014
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