Molecular Cloning and the Allergenic Characterization of Tropomyosin from Tyrophagus putrescentiae

Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine, Seoul, Korea.
Protein and Peptide Letters (Impact Factor: 1.07). 02/2007; 14(5):431-6. DOI: 10.2174/092986607780782777
Source: PubMed


Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.

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    • "It can vector pathogens, such as Aspergillus flavus Link and Fusarium poae (Peck) Wollenw to sterile grain and mushroom beds, and can also promote nonbeneficial fungal growth, increasing aflatoxin and deoxynivalenol production (Franzolin et al. 1999, Hubert et al. 2013b). T. putrescentiae infests the mycelia and fruiting bodies of edible mushrooms, decreasing production and causing mildews on dried mushrooms, human allergic dermatitis , pulmonary acariasis, and intestinal acariasis (Li et al. 2003, Jeong et al. 2007 ). The mite seriously affects both the economic efficiency of mushroom production and the health of mushroom growers (Zhang et al. 1992). "
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    • "Microorganisms growing on plant debris can supply the lacking nitrogen. Synanthropic mites are regarded as pests because they produce many compounds contaminating the indoor environment and cause allergic reaction in humans [5], [6]. The mites also interact and vector microorganisms of medical importance [7], [8]. "
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