Article

Molecular Cloning and the Allergenic Characterization of Tropomyosin from Tyrophagus putrescentiae

Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine, Seoul, Korea.
Protein and Peptide Letters (Impact Factor: 1.07). 02/2007; 14(5):431-6. DOI: 10.2174/092986607780782777
Source: PubMed

ABSTRACT

Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.

1 Follower
 · 
9 Reads
  • Source
    • "It can vector pathogens, such as Aspergillus flavus Link and Fusarium poae (Peck) Wollenw to sterile grain and mushroom beds, and can also promote nonbeneficial fungal growth, increasing aflatoxin and deoxynivalenol production (Franzolin et al. 1999, Hubert et al. 2013b). T. putrescentiae infests the mycelia and fruiting bodies of edible mushrooms, decreasing production and causing mildews on dried mushrooms, human allergic dermatitis , pulmonary acariasis, and intestinal acariasis (Li et al. 2003, Jeong et al. 2007 ). The mite seriously affects both the economic efficiency of mushroom production and the health of mushroom growers (Zhang et al. 1992). "
    [Show abstract] [Hide abstract]
    ABSTRACT: China is the largest producer, consumer, and exporter of mushrooms in the world. The storage mite, Tyrophagus putrescentiae Schrank, is one of the most important arthropod pests in mushroom cultivation. This study investigated the development and reproductive traits of this mite reared on four mushroom species: Agaricus bisporus Lange, Pleurotus ostreatus Kumm, Auricularia polytricha (Mont.) Sacc., and Flammulina velutipes (Fr.) Sing., at seven constant temperatures ranging from 16 to 34°C at 80% relative humidity. Development time for the immature stages decreased with increasing temperature, and was also significantly affected by mushroom species. The shortest immature developmental period (7.0 ± 0.2 d) was observed at 31°C when reared on F. velutipes, while the longest development was at 16°C (36.0 ± 0.3 d) reared on P. ostreatus. The effects of temperature and mushroom hosts on the development, female longevity, and reproduction were also significant. The lower threshold temperatures from egg-to-adult for the four mushroom species were 11.97, 12.02, 10.80, and 11.57°C, for A. bisporus, P. ostreatus, Au. polytricha, and F. velutipes, and the thermal constants were 133.3, 136.8, 165.2, and 135.9 degree days (°C d), for the same mushroom species, respectively. Life table parameters at 25°C were estimated as follows: net reproductive rates (R0), 59.16, 28.94, 42.62, and 62.93, and intrinsic rate of natural increase (rm), 0.24, 0.13, 0.17, and 0.24, respectively. These results suggest that these mushrooms are suitable hosts for T. putrescentiae, and the storage mite may be able to adapt to higher temperatures.
    Full-text · Article · Apr 2015 · Environmental Entomology
  • Source
    • "Microorganisms growing on plant debris can supply the lacking nitrogen. Synanthropic mites are regarded as pests because they produce many compounds contaminating the indoor environment and cause allergic reaction in humans [5], [6]. The mites also interact and vector microorganisms of medical importance [7], [8]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Tyrophagus putrescentiae (Acari: Astigmata) and Fusarium sp. co-occur in poorly managed grain. In a laboratory experiment, mite grazing resulted in significant reduction of fungal mycelium on cultivation plates. The destruction of mycelium appeared to be a result of an interaction between the mites, fungi and associated bacteria. A laboratory experiment was performed to simulate a situation of grain multiinfested by mites and Fusarium fungi. Changes of mite-associated bacterial community in T. putrescentiae were described in 3 habitats: (i) T. putrescentiae mites from a rearing diet prior to their transfer to fungal diet; (ii) fungal mycelium before mite introduction; (iii) mites after 7 day diet of each Fusarium avenaceum, F. culmorum, F. poae and F. verticillioides. Bacterial communities were characterized by 16 S rRNA gene sequencing. In total, 157 nearly full-length 16 S rRNA gene sequences from 9 samples representing selected habitats were analyzed. In the mites, the shift from rearing to fungal diet caused changes in mite associated bacterial community. A diverse bacterial community was associated with mites feeding on F. avenaceum, while feeding on the other three Fusarium spp. led to selection of a community dominated by Bacillaceae. The work demonstrated changes of bacterial community associated with T. putrescentiae after shift to fungal diets suggesting selection for Bacillaceae species known as chitinase producers, which might participate in the fungal mycelium hydrolysis.
    Full-text · Article · Oct 2012 · PLoS ONE
  • Source

    Full-text · Article · Jan 2009
Show more