Article

Detection and analysis of bovine foamy virus infection by an indicator cell line1

Laboratory of Molecular Virology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Acta Pharmacologica Sinica (Impact Factor: 2.91). 08/2007; 28(7):994-1000. DOI: 10.1111/j.1745-7254.2007.00563.x
Source: PubMed

ABSTRACT

To determine the infectivity and replication strategy of bovine foamy virus (BFV) in different cultured cells using the BFV indicator cell line (BICL) system.
BFV infection was induced by the co-culture method or the transient transfection of the infectious BFV plasmid [pCMV (cytomegalovirus) - BFV] clone. The infectivity of BFV was monitored by the percentage of green fluorescent protein-positive cells in the BICL. The effect of reverse transcriptase inhibitor zidovudine (AZT) on BFV replication was also evaluated in the BICL.
The titer of BFV in fetal bovine lung cells was 4-5-folds more than that in either 293T or HeLa (Cells from Henrietta lacks) cells using the co-culture method, and in the meantime was significantly higher than that produced by the infectious clone pCMV-BFV in the same cells. AZT had only a minor effect on viral titers when added to cells prior to the virus infection. In contrast, viral titers reduced sharply to the level of the negative control when the virus was produced from cells in the presence of AZT.
BICL can be used for the titration of the BFV viral infection in non-cytopathic condition. In addition, we provide important evidence to show that reverse transcription is essential for BFV replication at a late step of viral infection.

Full-text preview

Available from: nature.com
  • [Show abstract] [Hide abstract]
    ABSTRACT: Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BIV long terminal repeat was constructed. The BIV indicator cell line, designated BIVE, can detect BIV infection more easily and effectively than the established method, which involves the observation of cell cytopathic effects. Furthermore, viral titration using an assay based on the indicator cells is 100 times more sensitive than the assay based on cytopathic effect. The finding that BIV can infect the hamster cell line expands the known host range of BIV in vitro. The BIV indicator cell line could also be used for the evaluation of the inhibitory effect of antiviral agents. The fusion inhibition effect of the heptad repeat 2 region of the BIV envelope protein could also be quantified.
    No preview · Article · Aug 2009 · Journal of virological methods
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Foamy viruses (FVs) are complex retroviruses under the genus Spumavirus of family Retroviridae. They cause induction of multinucleated giant cell formation which presents numerous vacuoles, giving the monolayer culture a foamy appearance. FVs can infect animals as well as humans. In case of the human foamy virus (HFV), a defective variant (named ΔHFV or HFVΔTas) negatively interferes with replication of parental counterpart. Some species, such as rhesus macaques, African green monkeys, chimpanzees and cats harbor closely related yet serologically distinct FV subtypes. Unanticipated FV pathogenicity may warrant appropriate attention to biosafety practices to prevent occupational infections, and the importance of additional studies to better define clinical outcome of these zoonotic infections. During cross-species infection and subsequent passages a rapid and fatal disease can occur, with changes from nonpathogenic to pathogenic potentials. In persons occupationally exposed to non-human primates, simian foamy virus (SFV) infection occurs persistently showing that simian retroviruses cross into humans more frequently. Simian immunodeficiency viruses (SIV), mostly are nonpathogenic in their natural hosts but during cross-species infection a rapid and fatal disease can occur. Enzyme immuno assay (EIA), Western blot analysis, and polymerase chain reaction (PCR) amplification are the important diagnostic tests for FVs. FVs are also being exploited as potential vectors that can be used for gene therapy which is gaining much attention of the researchers worldwide. Strengthening sero-epidemiological as well as molecular investigations, and public health surveillance programme along with extra precautions while transferring xenograft are some of the approaches to prevent these viral infections.
    Full-text · Article · Apr 2014 · Asian Journal of Animal and Veterinary Advances