Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene

Iwate University, Morioka, Iwate, Japan
Plant Cell Reports (Impact Factor: 3.07). 12/2007; 26(11):1967-75. DOI: 10.1007/s00299-007-0404-x
Source: PubMed


We transformed a construct containing the sense coat protein (CP) gene of Soybean dwarf virus (SbDV) into soybean somatic embryos via microprojectile bombardment to acquire SbDV-resistant soybean plants. Six independent T(0) plants were obtained. One of these transgenic lines was subjected to further extensive analysis. Three different insertion patterns of Southern blot hybridization analysis in T(1) plants suggested that these insertions introduced in T(0) plants were segregated from each other or co-inherited in T(1) progenies. These insertions were classified into two types, which overexpressed SbDV-CP mRNA and accumulated SbDV-CP-specific short interfering RNA (siRNA), or repressed accumulation of SbDV-CP mRNA and siRNA by RNA analysis prior to SbDV inoculation. After inoculation of SbDV by the aphids, most T(2) plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA. We discuss here the possible mechanisms of the achieved resistance, including the RNA silencing.

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    • "Efforts have been made to improve the quality and quantity of macronutrients such as protein/amino acids, oils/fats and carbohydrates as well as to increase the bioavailability of micronutrients, vitamins and antioxidants through transgenics and breeding. In soya bean, most of the work was carried out to improve seed protein quality (Cunha et al., 2011;Nishizawa et al., 2010;Qi et al., 2011), oil content (Kajikawa et al., 2008;Li et al., 2010), biotic resistance (Cunha et al., 2010;Dang and Wei, 2007;McLean et al., 2007;Tougou et al., 2007) and abiotic resistance (DeRonde et al., 2004;Valente et al., 2009). However, on the other hand, there have been limited efforts in the management of antinutrients and/or toxins, which are the byproducts of plant metabolism and considered as health hazards to humans and animals. "
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    ABSTRACT: Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known precursor of β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), a neurotoxin found in grass pea. Here, we report the reduction in OA level in soya bean (up to 73%) and grass pea (up to 75%) seeds by constitutive and/or seed-specific expression of an oxalate-degrading enzyme, oxalate decarboxylase (FvOXDC) of Flammulina velutipes. In addition, β-ODAP level of grass pea seeds was also reduced up to 73%. Reduced OA content was interrelated with the associated increase in seeds micronutrients such as calcium, iron and zinc. Moreover, constitutive expression of FvOXDC led to improved tolerance to the fungal pathogen Sclerotinia sclerotiorum that requires OA during host colonization. Importantly, FvOXDC-expressing soya bean and grass pea plants were similar to the wild type with respect to the morphology and photosynthetic rates, and seed protein pool remained unaltered as revealed by the comparative proteomic analysis. Taken together, these results demonstrated improved seed quality and tolerance to the fungal pathogen in two important legume crops, by the expression of an oxalate-degrading enzyme.
    Full-text · Article · Jan 2016 · Plant Biotechnology Journal
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    • "Advancements in epigenetic research have enabled researchers to unravel the molecular events in virus-infected plants. Plants systematically utilize the siRNA-mediated methylation strategy as defence mechanism towards various viruses, by methylating various viral genomic components such as intergenic and transcribed region (Bian et al. 2006; Tougou et al. 2007; Yadav and Chattopadhyay 2011; Emran et al. 2012; Sharma et al. 2012). Rodríguez-Negrete et al. (2009) correlated the symptoms of recovery after viral infection with a higher proportion of viral DNA methylation . "
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    ABSTRACT: Epigenetics has become one of the hottest topics of research in plant functional genomics since it appears promising in deciphering and imparting stress-adaptive potential in crops and other plant species. Recently, numerous studies have provided new insights into the epigenetic control of stress adaptation. Epigenetic control of stress-induced phenotypic response of plants involves gene regulation. Growing evidence suggest that methylation of DNA in response to stress leads to the variation in phenotype. Transposon mobility, siRNA-mediated methylation and host methyltransferase activation have been implicated in this process. This review presents the current status of epigenetics of plant stress responses with a view to use this knowledge towards engineering plants for stress tolerance.
    Full-text · Article · May 2013 · Plant Cell Reports
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    ABSTRACT: We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T(1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.
    No preview · Article · Mar 2009 · Plant Cell Reports
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