Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene

ArticleinPlant Cell Reports 26(11):1967-75 · December 2007with9 Reads
DOI: 10.1007/s00299-007-0404-x · Source: PubMed
Abstract
We transformed a construct containing the sense coat protein (CP) gene of Soybean dwarf virus (SbDV) into soybean somatic embryos via microprojectile bombardment to acquire SbDV-resistant soybean plants. Six independent T(0) plants were obtained. One of these transgenic lines was subjected to further extensive analysis. Three different insertion patterns of Southern blot hybridization analysis in T(1) plants suggested that these insertions introduced in T(0) plants were segregated from each other or co-inherited in T(1) progenies. These insertions were classified into two types, which overexpressed SbDV-CP mRNA and accumulated SbDV-CP-specific short interfering RNA (siRNA), or repressed accumulation of SbDV-CP mRNA and siRNA by RNA analysis prior to SbDV inoculation. After inoculation of SbDV by the aphids, most T(2) plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA. We discuss here the possible mechanisms of the achieved resistance, including the RNA silencing.
    • "Efforts have been made to improve the quality and quantity of macronutrients such as protein/amino acids, oils/fats and carbohydrates as well as to increase the bioavailability of micronutrients, vitamins and antioxidants through transgenics and breeding. In soya bean, most of the work was carried out to improve seed protein quality (Cunha et al., 2011; Nishizawa et al., 2010; Qi et al., 2011), oil content (Kajikawa et al., 2008; Li et al., 2010), biotic resistance (Cunha et al., 2010; Dang and Wei, 2007; McLean et al., 2007; Tougou et al., 2007) and abiotic resistance (DeRonde et al., 2004; Valente et al., 2009). However, on the other hand, there have been limited efforts in the management of antinutrients and/or toxins, which are the byproducts of plant metabolism and considered as health hazards to humans and animals. "
    [Show abstract] [Hide abstract] ABSTRACT: Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known precursor of β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), a neurotoxin found in grass pea. Here, we report the reduction in OA level in soya bean (up to 73%) and grass pea (up to 75%) seeds by constitutive and/or seed-specific expression of an oxalate-degrading enzyme, oxalate decarboxylase (FvOXDC) of Flammulina velutipes. In addition, β-ODAP level of grass pea seeds was also reduced up to 73%. Reduced OA content was interrelated with the associated increase in seeds micronutrients such as calcium, iron and zinc. Moreover, constitutive expression of FvOXDC led to improved tolerance to the fungal pathogen Sclerotinia sclerotiorum that requires OA during host colonization. Importantly, FvOXDC-expressing soya bean and grass pea plants were similar to the wild type with respect to the morphology and photosynthetic rates, and seed protein pool remained unaltered as revealed by the comparative proteomic analysis. Taken together, these results demonstrated improved seed quality and tolerance to the fungal pathogen in two important legume crops, by the expression of an oxalate-degrading enzyme.
    Full-text · Article · Jan 2016
    • "Additionally, they contained SbDV-CPspecific small interfering RNAs (siRNAs) suggesting that the T 2 plants acquired resistance to SbDV through RNA silencing-mediated process (Tougou et al., 2006). The T 2 progenies derived from soybeans transformed with positive-sense SbDV-CP gene remained symptomless after inoculation with SbDV through aphids and additionally showed little SbDV-specific RNA (Tougou et al., 2007). These results show good prospects for generating genetically engineered SbDV-resistant soybean cultivars. "
    [Show abstract] [Hide abstract] ABSTRACT: Diverse array of food legume crops (Fabaceae: Papilionoideae) have been adopted worldwide for their protein-rich seed. Choice of legumes and their importance vary in different parts of the world. The economically important legumes are severely affected by a range of virus diseases causing significant economic losses due to reduction in grain production, poor quality seed, and costs incurred in phytosanitation and disease control. The majority of the viruses infecting legumes are vectored by insects, and several of them are also seed transmitted, thus assuming importance in the quarantine and in the epidemiology. This review is focused on the economically important viruses of soybean, groundnut, common bean, cowpea, pigeonpea, mungbean, urdbean, chickpea, pea, faba bean, and lentil and begomovirus diseases of three minor tropical food legumes (hyacinth bean, horse gram, and lima bean). Aspects included are geographic distribution, impact on crop growth and yields, virus characteristics, diagnosis of causal viruses, disease epidemiology, and options for control. Effectiveness of selection and planting with virus-free seed, phytosanitation, manipulation of crop cultural and agronomic practices, control of virus vectors and host plant resistance, and potential of transgenic resistance for legume virus disease control are discussed.
    Full-text · Article · Nov 2014
    • "Tougou et al. (2007) concluded that the level of transcript between homozygous and hemizygous had affected the degree of resistance in soybean against soybean dwarf virus. A small change in the transgene by methylation may affect the viral resistance via PTGS (Tougou et al. 2007). Another reason could be the implicated RNA threshold models for gene silencing at the level of gene expression rather than transgene copy number level (Meins and Kunz 1994; and Chareonpornwattana et al. 1999). "
    [Show abstract] [Hide abstract] ABSTRACT: The Yellow mosaic disease is caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus of the family Geminiviridae. Yellow mosaic disease (YMD) is a major constraint to the production of soybean in South-East Asia. In India, yield losses of 10–88% had been reported due to YMD of soybean. An effort has been made to generate resistant soybean plants, by a construct targeting replication initiation protein (Rep) gene sequences of MYMIV. A construct containing the sequences of Rep gene (566 bp) in antisense orientation was used to transform cotyledonary node explants of three soybean cultivars (JS 335, JS 95-60 and NRC 37). Transformation efficiencies of 0.2, 0.21 and 0.24% were obtained with three soybean cultivars, JS 335, JS 95-60 and NRC 37, respectively. The presence of transgene in T1 plants was confirmed by polymerase chain reaction (PCR) and sequence analysis. The level of resistance was observed by challenge inoculation with the virus in T1 lines. The inheritance of transgene showed classical Mendelian pattern in six transgenic lines.
    Full-text · Article · Nov 2013
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