Defined reprogramming: A vehicle for changing the differentiated state

Article · Literature ReviewinDifferentiation 75(7):577-9 · October 2007with12 Reads
DOI: 10.1111/j.1432-0436.2007.00213.x · Source: PubMed
  • ... Most human iPSCs derive from fibroblasts and they are reprogrammed using the forced expression of transcription factors, which are known oncogenes[37]. For this reason, the clinical application of iPSCs is currently being investigated with great caution, given the high potential for oncogenicity that may result from the use of these factors[38]. However, it should be noticed that the use of iPSCs rather than hESCs avoids an ethical issue since the genetic information contained in iPSCs derives from the patient's own genome and is also less likely to induce an immune response[39]. ...
  • ... Takahashi and Yamanaka in 2006 first reported the [4] concept of induced pluripotent stem cells (iPS cells) . However because of the teratogenic potential of induced pluripotent stem cells, the technique is yet in the frame of [5,6] question mark for its commercial use in the mass . However, in contrast, a variety of multipotent adult stem cells exist in almost all tissues of the organisms which reside in a specific niche in vivo where various microenvironmental cues form an intertwined signaling regulatory network that maintains stem cells fate and functions. ...
  • ... If they are injected in an undifferentiated state, they cause teratomas, and mice generated from iPS cells show high rates of tumors. This oncogenicity may be due to the transcription factors used for dedifferentiation which are known to be oncogenes, due to the insufficient epigenetic remodeling or due to the oncogenic retroviruses used for transfection [15]. The use of adult stem cells raises less ethical concerns and has proved to be much safer than pluripotent stem cells. ...
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