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Microbial biosensors

Division of Chemical and Biomolecular Engineering and Centre of Biotechnology, Nanyang Technological University, Singapore 637722, Singapore.
Analytica chimica acta (Impact Factor: 4.51). 06/2006; 568(1-2):200-10. DOI: 10.1016/j.aca.2005.11.065
Source: PubMed

ABSTRACT

A microbial biosensor is an analytical device that couples microorganisms with a transducer to enable rapid, accurate and sensitive detection of target analytes in fields as diverse as medicine, environmental monitoring, defense, food processing and safety. The earlier microbial biosensors used the respiratory and metabolic functions of the microorganisms to detect a substance that is either a substrate or an inhibitor of these processes. Recently, genetically engineered microorganisms based on fusing of the lux, gfp or lacZ gene reporters to an inducible gene promoter have been widely applied to assay toxicity and bioavailability. This paper reviews the recent trends in the development and application of microbial biosensors. Current advances and prospective future direction in developing microbial biosensor have also been discussed.

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Available from: Yu Lei, Mar 12, 2014
    • "Recently, whole-cell microbial biosensors were developed to detect various toxic metal ions (Lei et al., 2006). The regulatory elements of heavy metal resistance operons isolated from several bacterial strains have been harnessed to construct bacterial biosensors for heavy metals such as cadmium and lead (Raja and Selvam, 2011; Shetty et al., 2003). "
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    ABSTRACT: Multiple copies of a cadC homolog encoding a heavy metal-responsive transcription factor were found in the genome of a bacterium isolated from ocean sediment, and the heavy metal responses of the encoded proteins were characterized using a fluorescence reporter assay. Each CadC regulator exhibited distinct specificity in response to heavy metal ions, indicating their potential use as modular heavy metal biosensors. Next, we constructed CadC-controlled T7 RNA transcription systems for intracellular signal amplification, i.e., higher sensitivity. Flow cytometry revealed that cadmium and lead ions could be recognized specifically by CadC-T7 biosensors, which could be combined with a microfluidic platform to generate heavy metal biosensor devices with increased sensitivity. Our results demonstrate the successful development of synthetic CadC-T7 genetic circuitry for use in improved heavy metal biosensor microfluidic devices. http://dx.doi.org/10.1016/j.bios.2015.12.101
    No preview · Article · May 2016 · Biosensors & Bioelectronics
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    • "Such biosensors can be used in environmental monitoring and control[7], and toxicity tests[2,89101112. Among many different types of recognition elements (e. g. enzymes, antibodies, receptors, micro-organisms, animal or plant cells and tissues), the micro-organisms have some advantages including sensitivity towards various chemical substances for a wide range of pH and temperature[13]. Yeast cells are attractive as a recognition element of cellbased biosensors, because they can remain viable in adverse conditions especially if they are immobilized on membranes and hydrogels[14]. "
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    ABSTRACT: Yeast cells Saccharomyces cerevisiae were investigated by scanning electrochemical microscopy (SECM) at generation-collection mode. A two redox mediator based system was designed and adapted in order to evaluate redox activity of yeast cells. One redox mediator was 9,10-phenanthrenequinone (PQ), which was lipophilic and was incorporated within the cell membrane. The second redox mediator was K3[Fe(CN)6] (potassium ferricyanide), which is hydrophilic and was acting as an electron shuttle between PQ, which was incorporated within cell membrane, and the SECM electrode. The most efficient concentrations of redox mediators suitable for the visualization of the redox process of yeast cells were determined as 0.6 mmol L-1 for potassium ferricyanide and 0.04 mmol L-1 for PQ in the presence of 60 mmol L-1 of glucose.
    Full-text · Article · Jan 2016 · Sensors and Actuators B Chemical
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    • "In contrast to enzyme-based biosensors, whole-cell biosensors are more resistant to loss of activity as their many enzymes and cofactors are optimized by nature. On top of that, their co-existence results in the ability of the cells to cooperate and compromise their selectivity in detecting the different types of pollutants in the environment [18] [19]. These features make them the ideal candidates for incorporation into biosensors. "

    Full-text · Article · Jul 2015
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