Transcriptional profiling of chickpea genes differentially regulated in response to high-salinity, cold and drought. BMC Genomics 8:303

RMIT University, School of Applied Sciences, Biotechnology and Environmental Biology, Building 223, Level 1, Plenty Road, Bundoora, Victoria 3083, Australia.
BMC Genomics (Impact Factor: 3.99). 02/2007; 8(1):303. DOI: 10.1186/1471-2164-8-303
Source: PubMed


Cultivated chickpea (Cicer arietinum) has a narrow genetic base making it difficult for breeders to produce new elite cultivars with durable resistance to major biotic and abiotic stresses. As an alternative to genome mapping, microarrays have recently been applied in crop species to identify and assess the function of putative genes thought to be involved in plant abiotic stress and defence responses. In the present study, a cDNA microarray approach was taken in order to determine if the transcription of genes, from a set of previously identified putative stress-responsive genes from chickpea and its close relative Lathyrus sativus, were altered in chickpea by the three abiotic stresses; drought, cold and high-salinity. For this, chickpea genotypes known to be tolerant and susceptible to each abiotic stress were challenged and gene expression in the leaf, root and/or flower tissues was studied. The transcripts that were differentially expressed among stressed and unstressed plants in response to the particular stress were analysed in the context of tolerant/susceptible genotypes.
The transcriptional change of more than two fold was observed for 109, 210 and 386 genes after drought, cold and high-salinity treatments, respectively. Among these, two, 15 and 30 genes were consensually differentially expressed (DE) between tolerant and susceptible genotypes studied for drought, cold and high-salinity, respectively. The genes that were DE in tolerant and susceptible genotypes under abiotic stresses code for various functional and regulatory proteins. Significant differences in stress responses were observed within and between tolerant and susceptible genotypes highlighting the multiple gene control and complexity of abiotic stress response mechanism in chickpea.
The annotation of these genes suggests that they may have a role in abiotic stress response and are potential candidates for tolerance/susceptibility.

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Available from: Rebecca Ford, Jan 08, 2014
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    • "By combining these different datasets, a hybrid assembly with 46 369 transcript assembly contigs has been developed from RNA samples from >22 tissues representing a range of developmental stages and eight tissues challenged by different stresses collected from 17 different chickpea genotypes (Kudapa et al. 2014). Many studies reported several genes or ESTs to be involved in various stress responses based on transcriptomic and proteomic studies (Pandey et al. 2006, 2008; Mantri et al. 2007; Molina et al. 2008, 2011; Varshney et al. 2009a). However, gene discovery has been very limited in chickpea. "
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    • "legumes after beans and field beans in the world. It is considered a droughtresistant crop and is therefore mostly grown in arid and semiarid areas where salinity is a common problem (Saxena, 1984; Nitin et al., 2007). The crop is basically adapted to a neutral pH, and the sensitivity of legumes, including chickpea, to salinity is well documented (Marschner, 1995; Wang et al., 2003). "
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    • "From an agricultural point of view, such stresses are the most significant causes of losses in crop production that can be both substantial and unpredictable . The physiological mechanisms governing plant responses to salinity and drought are similar suggesting that both stresses are perceived by the plant cell as water deprivation (Mantri et al. 2007). High salt concentrations in the soil reduce water potential and consequently decrease water availability (Hasegawa et al. 2000, Munns and Tester 2008, Jenkins et al. 2012). "
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