Zhang, L. et al. A robust immunoassay for anti-interferon autoantibodies that is highly specific for patients with autoimmune polyglandular syndrome type 1. Clin. Immunol. 125, 131-137

Department of Medicine, University of California, San Francisco, San Francisco, California, United States
Clinical Immunology (Impact Factor: 3.67). 11/2007; 125(2):131-7. DOI: 10.1016/j.clim.2007.07.015
Source: PubMed


High titer antibodies to type 1 interferons have been recently reported as being highly specific for patients with autoimmune polyglandular syndrome type 1 (APS1) in Finnish and Norwegian patients with mutations in the AIRE gene. Those studies employed a complex neutralization assay to define the type 1 interferon autoantibodies. Here we have established a competitive europium time resolved fluorescence assay for IFN-α autoantibodies and measured sera from subjects with APS1, first degree relatives of APS1 patients, patients with Addison’s disease or Type 1 diabetes. The europium-based immunoassay utilizes plate bound human IFN-α incubated with sera with or without competition with fluid phase IFN-α, followed by anti-IgG biotinylated antibody and detection with streptavidin-europium. The index of IFN-α Ab was calculated as (CPS (Counts per second) without competition − CPS with competition) / (CPS positive standard sera without competition − CPS positive standard sera with competition). Results are reported for raw CPS and indices and are compared across the different subjects.

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