Metallothionein responses in the Asiatic clam (Corbicula fluminea) after exposure to trivalent arsenic. Biomarkers

Instituto do Mar, Dept de Ciências e Engenharia do Ambiente, Caparica, Portugal.
Biomarkers (Impact Factor: 2.26). 11/2007; 12(6):589-98. DOI: 10.1080/13547500701507701
Source: PubMed


The main objective of this work was to evaluate arsenic effects on metallothionein (MT) induction by exposing a freshwater Asiatic clam (Corbicula fluminea) to different concentrations of this metalloid. The presence of MT-like proteins was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis and compared with a standard rabbit MT. In addition, the polarographic response showed good correspondence between standard MT and MT-like curves from C. fluminea, allowing MT quantification. The results show that clams exposed to different concentrations of arsenic are able to induce significant levels of MTs. Although variability was found in MT induction, significant differences in MT levels were found after 28 days of exposure in all treatments in comparison with the controls, suggesting that exposure to arsenic induced MT-like proteins in C. fluminea.

Download full-text


Available from: Pedro M Costa, Jul 10, 2014
  • Source
    • "responding to water contamination, for instance, in the national French program ECODYN (Diniz et al., 2007). Furthermore , C. fluminea is a convenient model in toxicological studies provided the mentioned features as well as considering that the species is of easy maintenance and testing under laboratory conditions, and can be used as an adequate representative of freshwater benthic bivalves. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The Asian clam Corbicula fluminea is an invasive bivalve that has recently spread in Europe and currently represents a large portion of the aquatic biomass in specific areas. Because of the impacts that the species may have in invaded ecosystems, increased knowledge on the physiologic features of the species life-cycle under different environmental scenarios (e.g., contamination events) is critical to understand the dynamics of the invasion and resulting ecosystem imbalance. The presence of pharmaceutical residues in the aquatic environment has recently received great attention since high levels of contamination have been found, not only in sewage treatment plant effluents, but also in open waters. The present article reports toxicological biochemical effects of paracetamol to Corbicula fluminea following short- and long-term exposures. Oxidative stress parameters were specially focused namely catalase (CAT), glutathione S-transferases (GSTs), and glutathione reductase (GRed). The effect of tested substances on lipid peroxidation was also investigated. Paracetamol did not induce alterations on CAT activity, caused a significant decrease of GSTs activity following short- and long-term exposure (LOEC values of 532.78 mg L(-1) and 30.98 μg L(-1) , respectively), and was responsible for a significant and dose-dependent decrease of GRed activity in short- and long-term exposures. These results indicate that exposure to paracetamol can provoke significant alterations on the cellular redox status of C. fluminea. 2011 Wiley Periodicals, Inc. Environ Toxicol, 2011.
    Full-text · Article · Jan 2014 · Environmental Toxicology
  • Source
    • "Marine organisms, in general, accumulate contaminants from the ambient environment, and therefore, have been extensively used in marine pollution monitoring programmes (UNEP, 1993). The effect of metals at sub-cellular and biochemical levels had been studied in several marine organisms (Diniz et al., 2007). When accumulated, metals in tissues reach the threshold level, detoxification mechanisms may be activated in order to prevent toxicity to the organisms. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Tissue samples of marine organisms from the coastal w aters of Agatti Island were subjected to analysis of metals (cadmium, cobalt, copper, iron, magnesium, manganese, nickel, lead and zinc), using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) for the assessment of the present condition of the Island ecosystem and comp iling the baseline data for future monitoring, with respect of metal accu-mulation of marine orga nisms. Tissue samples of fish, shellfish and seaweed revealed that the metals have different levels of accumulation viz. Cd = 0.08–0.1 4, Co = 0.01–0.02, Cu = 0.16–0.98, Fe = 3.19–5.3, Mg = 86.73–152.45, Mn = 0.17–0.55, Ni = 0.06–0.26, Pb = 0.11–0.46 and Zn = 3.26–14.2 lgg �1 dry wt. Metal concentrations were more in shellfish and less in finfish. Concentrations of toxic metals such as Cd, Co, Ni and Pb were well below the permissible limits proposed by the World Health Organization.
    Full-text · Article · Apr 2012 · Food Chemistry
  • Source
    • "The high affinity of arsenic for cellular debris, which includes tissue, cell membranes and intact cell fractions, has been attributed to the resemblance of arsenate to phosphate, which facilitates the binding of arsenic in phospholipids that constitute cell membranes (Vijver et al. 2006), whereas the preferential binding of arsenic to the MT fraction, which includes proteins like metallothionein, tripeptides as glutathione and other small proteins containing sulphidryl-rich aminoacids (Wallace et al. 2003), is attributed to the affinity of arsenic for sulphur in glutathione in the cytosol as previously demonstrated for terrestrial oligochaetes (Langdon et al. 2005). Terrestrial oligochaetes (Langdon et al. 2005) and freshwater clams Corbicula fluminea (Diniz et al. 2007) are also capable of inducing MT after arsenic assimilation. In this study we could observe a net increase in the concentration of arsenic in the MRG and the MT fractions after removal from arsenic exposure (Table 1). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The accumulation, subcellular distribution and speciation of arsenic in the polychaete Arenicola marina were investigated under different laboratory exposure conditions representing a range of metal bioavailabilities, to gain an insight into the physiological mechanisms of how A. marina handles bioaccumulated arsenic and to improve our understanding of the potential ecotoxicological significance of bioaccumulated arsenic in this deposit-feeder. The exposure conditions included exposure to sublethal concentrations of dissolved arsenate, exposure to sublethal concentrations of sediment-bound metal mining mixtures, and exposure to lethal concentrations of sediment-bound metal mining mixtures and arsenic- and multiple metal-spiked sediments. The sub-lethal exposures indicate that arsenic bioaccumulated by the deposit-feeding polychaete A. marina is stored in the cytosol as heat stable proteins (~50%) including metallothioneins, possibly as As (III)-thiol complexes. The remaining arsenic is mainly accumulated in the fraction containing cellular debris (~20%), with decreasing proportions accumulated in the metal-rich granules, organelles and heat-sensitive proteins fractions. A biological detoxified metal compartment including heat stable proteins and the fraction containing metal-rich granules is capable of binding arsenic coming into the cells at a constant rate under sublethal arsenic bioavailabilities. The remaining arsenic entering the cell is bound loosely into the cellular debris fraction, which can be subsequently released and diverted to an expanding detoxified pool. Our results suggest that a metal sensitive compartment comprising the cellular debris, enzymes and organelles fractions may be more representative of the toxic effects observed.
    Full-text · Article · Nov 2011 · Ecotoxicology
Show more