Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node

Départment d'Immunologie, Institut Pasteur, Paris, France.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2007; 380:355-63. DOI: 10.1007/978-1-59745-395-0_22
Source: PubMed


Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.

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