Surface reaction of Bacillus cereus biomass and its biosorption for lead and copper ions

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Journal of Environmental Sciences (Impact Factor: 2). 02/2007; 19(4):403-8. DOI: 10.1016/S1001-0742(07)60067-9
Source: PubMed


In this study, the surface chemical functional groups of Bacillus cereus biomass were identified by Fourier transform infrared (FTIR) analytical technique. It had been shown that the B. cereus cells mainly contained carboxyl, hydroxyl, phosphate, amino and amide functional groups. The potentiometric titration was conducted to explain the surface acid-base properties of aqueous B. cereus biomass. The computer program FITEQL 4.0 was used to perform the model calculations. The optimization results indicated that three sites-three pKas model, which assumed the cell surface to have three distinct types of surface organic functional groups based on the IR analysis results, simulated the experimental results very well. Moreover, batch adsorption experiments were performed to investigate biosorption behavior of Cu(II) and Pb(II) ions onto the biomass. Obviously, the adsorption equilibrium data for the two ions were reasonably described by typical Langmuir isotherm.

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    • "The cause of these shifts is difficult to be determined, but the results revealed interactions between the Pb(II) ions, hydroxyl, amino and carboxyl groups on the biomass surface similar to those reported by other authors [8] [13] [23]. "
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    • "The shifting of wave number as well as disappearance and reappearance of spectral bands from raw biomass to metal-loaded biomass was used to illustrate the binding of metal ions on biomass surface. Fourier transform infrared (FTIR) spectroscopy results revealed that carboxylic, amine, amide, phosphate, hydroxyl, carbonyl , phosphoryl, sulphonate, aldehyde, and amide sites of bacterial cells are the key functional groups for metal ion interaction (Guo et al. 2012; Hasan et al. 2012; Masood and Malik 2011; Aryal et al. 2010; Gabr et al. 2008; Jian-hua et al. 2007; Kang et al. 2007; Tunali et al. 2006; Sar et al. 1999). Oliveira et al. (2014) showed that alginate carboxylate groups are responsible for La(III) complexation on Sargassum sp. "
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    • "The peak at 1633.4 cm À 1 may attribute to functional groups such as CQC, CQO, and CQN. The band at 1426.7 cm À 1 is assigned to stretching of R–C–H groups whereas the band at 1373.6 assigned to stretching of R–C–H, C–O and C–N groups (Pan et al., 2007 "
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