Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Molecular &amp Cellular Proteomics (Impact Factor: 6.56). 02/2008; 7(1):163-9. DOI: 10.1074/mcp.M700115-MCP200
Source: PubMed


Dissociable antibody microarray (DAMA) staining is a technology that combines protein microarrays with traditional immunostaining techniques. It can simultaneously determine the expression and subcellular location of hundreds of proteins in cultured cells and tissue samples. We developed this technology and demonstrated its application in identifying potential biomarkers for breast cancer. We compared the expression profiles of 312 proteins among three normal breast cell lines and seven breast cancer cell lines and identified 10 differentially expressed proteins by the data analysis program DAMAPEP (DAMA protein expression profiling). Among those proteins, RAIDD, Rb p107, Rb p130, SRF, and Tyk2 were confirmed by Western blot and statistical analysis to have higher expression levels in breast cancer cells than in normal breast cells. These proteins could be potential biomarkers for the diagnosis of breast cancer.

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Available from: Wayne Zhou, Feb 27, 2015
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    • "Moreover, protein expression depends on different cellular and developmental conditions, as well as different cell types [43]. We used only one standard genomic sequence (UCSC hg19) and two different cell cultures not directly related to that genome. "
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    Full-text · Article · Feb 2013 · BMC Genomics
    • "Zhou et al.[41] developed dissociable antibody microarray (DAMA) staining technology that combines protein microarrays with traditional immune-staining techniques and demonstrated its application in identifying potential biomarkers for breast cancer. DAMA can simultaneously determine the expression and subcellular location of hundreds of proteins in cultured cells and tissue samples. "
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    ABSTRACT: There are more than 1.15 million cases of breast cancer diagnosed worldwide annually. At present, only small numbers of accurate prognostic and predictive factors are used clinically for managing the patients with breast cancer. DNA microarrays have the potential to assess the expression of thousands of genes simultaneously. Recent preliminary researches indicate that gene expression profiling based on DNA microarray can offer potential and independent prognostic information in patients with newly diagnosed breast cancer. In this paper, an overview upon the applications of microarray techniques in breast cancer is presented.
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    • "Recently, some cell (9) and tissue microarrays (10) have emerged for the study of gene activity, protein expression, and cell-surface exploration and offer the potential to accelerate high-throughput functional genetic studies. A new focus in the cell microarray field has been the and adaptation of cell arrays for RNAi in a high-throughput format (11, 12) and the development of a new protein microarray platform, Dissociable Antibody MicroArray (DAMA) staining allowing global analysis of protein expression and subcellular localization (13). Although cell microarray technology has many possible experimental uses, several technical limitations need to be addressed before its full potential can be fulfilled (8, 12, 14). "
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