The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection

Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Fertility and sterility (Impact Factor: 4.59). 08/2008; 90(1):60-4. DOI: 10.1016/j.fertnstert.2007.05.054
Source: PubMed


To investigate the association between the levels of two steroid hormones and eight cytokines in fluids from individual follicles and the fertilization outcome of the oocytes derived from the same follicles.
Prospective study.
University hospital.
Forty-three women participating in intracytoplasmic sperm injection (ICSI)/ET cycles.
The ovarian stimulation followed the multidose GnRH antagonist protocol. ICSI was performed in mature oocytes. The concentrations of estradiol, progesterone, tumor necrosis factor-alpha, interleukin (IL) -1beta, IL-6, vascular endothelial growth factor, leptin, basic fibroblast growth factor, epidermal growth factor, and insulin-like growth factor-I were measured by immunoassay methods in the follicles from which the mature oocytes were derived.
The concentrations of the above hormones and cytokines in individual follicles and the fertilization outcome of the oocytes derived from the same follicles.
The intrafollicular concentrations of the above factors were not significantly associated with the fertilization outcome. These factors were not correlated with embryo quality, with the exception of leptin, which was weakly associated with embryo score (R = 0.276).
The intrafollicular concentrations of the above factors cannot predict the fertilization outcome after ICSI.

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    • "Other studies suggested that leptin levels in FF could not be considered a marker of oocyte quality and developmental potential [111]. In recent studies, leptin was shown to be positively correlated with the fertilization rate [112], weekly associated with embryo morphological score, but not with the IVF outcome [61]. At present, leptin concentration in FF does not appear to precisely reflect oocyte quality and to be useful for oocyte selection. "
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