Yeast Cell-Surface Expression of Chitosanase from Paenibacillus fukuinensis

ArticleinBioscience Biotechnology and Biochemistry 71(11):2845-7 · December 2007with25 Reads
Impact Factor: 1.06 · DOI: 10.1271/bbb.70315 · Source: PubMed

    Abstract

    To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.