Impedance Spectroscopy and Biosensing

Biosystems Technology, Wildau University of Applied Sciences, Bahnhofstrasse 1, 15745 Wildau, Germany.
Advances in Biochemical Engineering/Biotechnology (Impact Factor: 1.66). 02/2008; 109:195-237. DOI: 10.1007/10_2007_081
Source: PubMed


This chapter introduces the basic terms of impedance and the technique of impedance measurements.
Furthermore, an overview of the application of this transduction method for analytical purposes will be
given. Examples for combination with enzymes, antibodies, DNA but also for the analysis of living cells
will be described. Special attention is devoted to the different electrode design and amplification schemes
developed for sensitivity enhancement. Finally, the last two sections will show examples from the label-free
determination of DNA and the sensorial detection of autoantibodies involved in celiac disease.

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    • "[2] [8] [9] [10] [11] "
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    ABSTRACT: To assist the design process of non-Faradic electrochemical sensors in realistic biological media, we examine multiple types of sensing operations in polyelectrolytes. By comparing the quasi-static transconductance, impedance spectroscopy, and capacitance-voltage (CV) measurements, we assess the physical contributions from the double layer composition, overall solution resistance, and sensing surface potential under various poly-electrolytic molarities. The mixture of NaCl and MgCl2 is chosen for illustration to provide insight into circuit model parameters for non-Faradaic sensing. Our finding also shed light on the dynamics of double-layer competition and correlation, which is critical for understanding the physical phenomena occurring at the sensing interface, and accurately interpreting sensor data.
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    • "The interdigitated electrodes allow the measurement of the change of conductivity in the region defined by field lines. The thickness probed by field lines is of the order of the interdigit distance (few tens of mm) (Pȁnke et al., 2008). As it has been modelled (Sheppard et al., 1996; Temple- Boyer et al., 2008), the observed steady-state response of the conductometric biosensor is the result of the reaction rate limited kinetics of the enzymatic reaction and the diffusive flux of urea hydrolysis products away from the transducer surface, in the boundary layer. "
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    ABSTRACT: Enzymatic conductometric biosensor, using immobilized Arthrospira platensis cells on gold interdigitated electrodes, for the detection of pesticides in water, was elaborated. Cholinesterase activity (AChE) was inhibited by pesticides and a variation of the local conductivity was measured after addition of the substrate acetylthiocholine chloride (AChCl). The Michaelis-Menten constant (Km) was evaluated to be 1.8 mM through a calibration curve of AChCl. Inhibition of AChE was observed with paraoxon-methyl, parathion-methyl, triazine and diuron with a detection limit of 10(-18) M, 10(-20) M, 10(-20) M and 10(-12) M, respectively and the half maximal inhibitory concentration (IC50) was determined at 10(-16) M, 10(-20) M, 10(-18) M and 10(-06) M, respectively. An important decrease of response time τ90% was recorded for AChE response towards AChCl after 30 min cell exposure to pesticides. Scanning electron microscopy images revealed a degradation of the cell surface in presence of pesticides at 10(-06) M.
    No preview · Article · Apr 2013 · Environmental Pollution
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    • "Electrochemical methods such as electrochemical impedance spectroscopy (EIS) are spreading rapidly due to their quick operation and simplicity in the field of chemical and biosensing [1]. Gold thin films are often used as working electrodes due to their several preferable properties, such as the relatively simple surface chemistry methods to chemically attach receptors on the electrode surface. "
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    ABSTRACT: In this work we investigate the CPE (constant phase element) behavior of gold thin films with EIS (electrochemical impedance spectroscopy). With results obtained on four technologically different gold thin films in different electrolytes we demonstrate that the consideration of capacitance dispersion of the working electrode is necessary for the precise determination of the double layer capacitance and charge transfer resistance in electrochemical sensor applications. We also investigate the effect of surface roughness of the electrodes on the CPE behavior.
    Full-text · Article · Oct 2011
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