Renal differentiation of amniotic fluid stem cells. Cell Prolif

Childrens Hospital Los Angeles, Saban Research Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
Cell Proliferation (Impact Factor: 3.12). 01/2008; 40(6):936-48. DOI: 10.1111/j.1365-2184.2007.00478.x
Source: PubMed


The role of stem cells in regenerative medicine is evolving rapidly. Here, we describe the application, for kidney regeneration, of a novel non-genetically modified stem cell, derived from human amniotic fluid. We show that these pluripotent cells can develop and differentiate into de novo kidney structures during organogenesis in vitro.
Human amniotic fluid-derived stem cells (hAFSCs) were isolated from human male amniotic fluid obtained between 12 and 18 weeks gestation. Green fluorescent protein and Lac-Z-transfected hAFSCs were microinjected into murine embryonic kidneys (12.5-18 days gestation) and were maintained in a special co-culture system in vitro for 10 days. Techniques of live microscopy, histology, chromogenic in situ hybridization and reverse transcriptase polymerase chain reaction were used to characterize the hAFSCs during their integration and differentiation in concert with the growing organ.
Green fluorescent protein and Lac-Z-transfected hAFSCs demonstrated long-term viability in organ culture. Histological analysis of injected kidneys revealed that hAFSCs were capable of contributing to the development of primordial kidney structures including renal vesicle, C- and S-shaped bodies. Reverse transcriptase polymerase chain reaction confirmed expression of early kidney markers for: zona occludens-1, glial-derived neurotrophic factor and claudin.
Human amniotic fluid-derived stem cells may represent a potentially limitless source of ethically neutral, unmodified pluripotential cells for kidney regeneration.

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Available from: Stefano Giuliani
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    • "Further, evidence indicated that transplanted allogeneic AFSCs have low immunogenicity and can escape immune rejection [19]. The potential advantages of AFSCs for treating POF are further suggested by their use in the studies of regenerative medicine to repair damaged tissues or organs, such as muscle [14], kidney [20], lung [21], or heart [22]. "
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    ABSTRACT: Chemotherapy used to treat cancer may cause irreversible premature ovarian failure (POF). Of late, amniotic fluid stem cells (AFSCs) provide a novel source for regenerative medicine because of their primitive stage, low immunogenicity, and easy accessibility. In this study, we isolated AFSCs from transgenic mice that ubiquitously express enhanced green fluorescence protein (EGFP). These AFSCs exhibited morphologies, immunophenotypes, and mesoderm trilineage differentiation potentials similar to mesenchymal stem cells (MSCs). Further, AFSCs proliferated faster than MSCs and expressed OCT4, a marker for pluripotency. To investigate their potential in recovering fertility in POF model, AFSCs were transplanted into the ovaries of mice with POF six weeks post induction using chemotherapeutic drugs, busulfan and cyclophosphamide. AFSCs could rescue the reproductive ability of mice with POF by preventing follicle atresia and sustaining the healthy follicles. Notably, the transplanted AFSCs did not differentiate into granulosa and germline cells in vivo. After one month, the decreased numbers of transplanted AFSCs accompanied with the reduced beneficial effects indicated that the therapeutic efficacy were directly from AFSCs. These findings demonstrated the therapeutic effects of AFSCs and suggested the promise of AFSCs for treating infertility and POF caused by chemotherapy.
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    • "There is a great controversy on HLA-I status in freshly isolated hAECs. Although according to some reports these cells express low levels of HLA-I immediately after isolation (8, 21, 22) there are some reports indicating that majority of these cell types express considerable amounts of HLA-I (15, 16). Equally important, in the studies reporting low levels of HLA-I on the isolated hACEs, the level of HLA-I expression has been found to be a function of culture period and after the first few passages, these cells expressed significant level of this cell surface antigen (22, 23). "
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    • "AFC contains many of the stem cells required for forming organs and epithelial tissues in early stage fetal development. Furthermore, it is known that applying AFC to fetal wounds allows for scarless healing, unlike the adult scar healing process [7-9]. "
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