Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res

Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon, Korea.
Transgenic Research (Impact Factor: 2.32). 09/2008; 17(4):705-15. DOI: 10.1007/s11248-007-9155-2
Source: PubMed


In plants, nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes. In this study, we developed transgenic potato plants (Solanum tuberosum L. cv. Atlantic) expressing Arabidopsis NDPK2 (AtNDPK2) gene in cytosols under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) or enhanced CaMV 35S promoter (EN plants) and evaluated their tolerance to various environmental stress, including methyl viologen (MV)-mediated oxidative stress, high temperature, and salt stress. When 250 muM MV was sprayed to whole plants, plants expressing NDPK2 showed significantly an enhanced tolerance compared to non-transgenic (NT) plants. SN plants and EN plants showed 51% and 32% less visible damage than NT plants, respectively. Transcript level of AtNDPK2 gene and NDPK2 activity in SN plants following MV treatment well reflected the plant phenotype. Ascorbate peroxidase (APX) activity was also increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to high temperature at 42 degrees C. The photosynthetic activity of SN plants after treatment of high temperature was decreased by about 10% compared to the plants grown at 25 degrees C, whereas that of NT plants declined by 30%. When treated with 80 mM NaCl onto the plantlets, both SN plants and EN plants also showed a significant reduced damage in root growth. These results indicate that overexpression of NDPK2 under the stress-inducible SWPA2 promoter might efficiently regulate the oxidative stress derived from various environmental stresses.

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Available from: Suk-Yoon Kwon, Dec 18, 2013
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    • "The expression of the GUS reporter gene driven by the SWPA2 promoter in transgenic tobacco plants is strongly induced in response to environmental stresses including H 2 O 2 , wounding and UV treatment (Kim et al., 2003). In addition, transgenic plants expressing various stress-tolerance genes under the control of the SWPA2 promoter exhibit increased tolerance to methyl viologen (MV)-induced oxidative stress as well as salt, drought, and temperature stresses (Tang et al., 2008; Kim et al., 2009, 2010, 2011). These results suggest that the SWPA2 promoter will be useful for the development of transgenic plants with enhanced tolerance to environmental stresses. "
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    • "Salt stress triggers oxidative stress in plant tissues; salt stress could reduce gas exchange and limit CO2 supply to the leaf [5], which causes an over-reduction of the photosynthetic electron transport chain [48] and concomitant production of ROS such as singlet oxygen (1O2), O2−, H2O2, and OH- [5], [49]. Thus, excessive ROS induced by salt stress must be removed in time to avoid plants suffered serious oxidative damage although they may also signal the induction of protection mechanisms [6], [50], [51]. In this study, we gradually increase the salt concentration to acclimate the plants to salt stress for cotton moderate tolerance [52], and our transgenic and control plants could flower and set seed. "
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    ABSTRACT: In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC, and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous genes (GhSOD1, GhAPX1, and GhCAT1) from Gossypium hirsutum. Overexpressing cassettes containing chimeric GhSOD1, GhAPX1, or GhCAT1 were introduced into cotton plants by Agrobacterium transformation, and overexpressed products of these genes were transported into the chloroplasts by transit peptide, as expected. The five types of transgenic cotton plants that overexpressed GhSOD1, GhAPX1, GhCAT1, GhSOD1 and GhAPX1 stack (SAT), and GhSOD1 and GhCAT1 stack (SCT) were developed. Analyses in the greenhouse showed that the transgenic plants had higher tolerance to methyl viologen (MV) and salinity than WT plants. Interestingly, SCT plants suffered no damage under stress conditions. Based on analyses of enzyme activities, electrolyte leakage, chlorophyll content, photochemical yield (Fv/Fm), and biomass accumulation under stresses, the SCT plants that simultaneously overexpressed GhSOD1 and GhCAT1 appeared to benefit from synergistic effects of two genes and exhibited the highest tolerance to MV and salt stress among the transgenic lines, while the SAT plants simultaneously overexpressing GhSOD1 and GhAPX1 did not. In addition, transgenic plants overexpressing antioxidant enzymes in their chloroplasts had higher tolerance to salt stress than those expressing the genes in their cytoplasms, although overall enzyme activities were almost the same. Therefore, the synergistic effects of GhSOD1 and GhCAT1 in chloroplasts provide a new strategy for enhancing stress tolerance to avoid yield loss.
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    • "It follows that there is similarity among many of the molecular entities that mediate ion homeostasis and salt stress signaling in all plants (Hasegawa et al. 2000b) as, for example, ion homeostasis that facilitates plant salt tolerance resembles that described for yeast (Bressan et al. 1998; Serrano et al. 1999). According to another finding of genomics, there is remarkable colinearity (gene synteny and homology) of gene sequences among different grass species, including cultivated crops (Tang et al. 2008; Zahn et al. 2008), and strongly suggests their resemblance in evolution and probably in functions. The facts stated above have made it feasible to use a model system for the dissection of the plant salt stress response (Bressan et al. 1998; Hasegawa et al. 2000a; Sanders et al. 1999; Serrano et al. 1999; Zhu 2000, 2001b). "

    Full-text · Chapter · Jan 2013
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