Wardle J, Burgers P M, Cann I K, et al. Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya. Nucleic Acids Res, 2008

Institute for Cell and Molecular Biosciences (ICaMB), University of Newcastle, Newcastle upon Tyne NE2 4HH, UK.
Nucleic Acids Research (Impact Factor: 9.11). 03/2008; 36(3):705-11. DOI: 10.1093/nar/gkm1023
Source: PubMed


Family B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures approximately 100 degrees C, specifically recognize uracil in DNA templates and stall replication in response to this base. Here it is demonstrated that interaction with uracil is not restricted to hyperthermophilic archaea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical behaviour. The family B DNA polymerases replicate the genomes of archaea, one of the three fundamental domains of life. This publication further shows that the DNA replicating polymerases from the other two domains, bacteria (polymerase III) and eukaryotes (polymerases delta and epsilon for nuclear DNA and polymerase gamma for mitochondrial) are also unable to recognize uracil. Uracil occurs in DNA as a result of deamination of cytosine, either in G:C base-pairs or, more rapidly, in single stranded regions produced, for example, during replication. The resulting G:U mis-pairs/single stranded uracils are promutagenic and, unless repaired, give rise to G:C to A:T transitions in 50% of the progeny. The confinement of uracil recognition to polymerases of the archaeal domain is discussed in terms of the DNA repair pathways necessary for the elimination of uracil.


Available from: Erik Johansson
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    • "Using these methods, only the PCR products that contain uracil are enzymatically digested; therefore, any contaminating PCR products can be digested with no risk of destroying the target DNA about to be amplified. Unfortunately, uracilated DNA is not amplified well with widely-used emulsion or cluster PCR kits, due to the use of uracil-illiterate polymerases in most next generation sequencing platforms [2]. "
    [Show abstract] [Hide abstract] ABSTRACT: We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a "DNA diode", where DNA can advance in a laboratory in only one direction and cannot feedback into upstream assays. Here we describe aspects of this method that enable consecutive amplification with the introduction of a 5th and 6th base while simultaneously providing methylation dependent mitochondrial DNA enrichment. These additional nucleotides enable a novel DNA decontamination technique that generates ephemeral and easy to decontaminate DNA.
    Full-text · Article · May 2014 · PLoS ONE
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    • "The catalytic core of yeast Pol d aligns with RB69 DNA Pol in the general plan of organization with unique structural features [Swan et al., 2009]. All Pols possess remnants of the uracil recognizing domain but do not sense uracil like their archael homologs [Wardle et al., 2008]. The C-terminal domain of Pols (CTD) has two cysteine-rich metal binding motifs (MBM1 and MBM2) critical for the assembly of the holoenzymes [Dua et al., 1998; Sanchez Garcia et al., 2004; Klinge et al., 2009; Tahirov et al., 2009]. "
    [Show abstract] [Hide abstract] ABSTRACT: The rate of mutations in eukaryotes depends on a plethora of factors and is not immediately derived from the fidelity of DNA polymerases (Pols). Replication of chromosomes containing the anti-parallel strands of duplex DNA occurs through the copying of leading and lagging strand templates by a trio of Pols α, δ and ϵ, with the assistance of Pol ζ and Y-family Pols at difficult DNA template structures or sites of DNA damage. The parameters of the synthesis at a given location are dictated by the quality and quantity of nucleotides in the pools, replication fork architecture, transcription status, regulation of Pol switches, and structure of chromatin. The result of these transactions is a subject of survey and editing by DNA repair. Environ. Mol. Mutagen., 2012. © 2012 Wiley Periodicals, Inc.
    Full-text · Article · Dec 2012 · Environmental and Molecular Mutagenesis
    • "Here we present one more example of site-directed chemical modification selectively changing enzyme properties. The archaeal DNA polymerases of family B have N-terminally located highly conserved U-binding pocket that carries out a template-checking function during replication [9][10][11]. Mutations of key-role amino acids in U-binding pocket of the polymerase confer the ability to read through template-strand uracils [9,13] . "
    [Show abstract] [Hide abstract] ABSTRACT: We present site-directed chemical modification (SDCM), a tool for engineering U-resistant archaeal DNA polymerases of family B. The Thermococcus litoralis Sh1B DNA polymerase (GenBank: GQ891548) was chosen as the object of the study. Similar to D.Tok, Kod1, Pfu, Tgo and other archaeal members of this family, the T. litoralis Sh1B DNA polymerase is a domain structured, proofreading-proficient enzyme that has the polymerization and 3'-->5' DNA exonucleolytic activities and contains N-terminally located highly conserved template-strand U-binding pocket. The tight binding of template uracil in the enzyme pocket during polymerization blocks the replication of DNA containing uracils. This effect can be alleviated by mutations in key amino acids of the U-binding pocket. We altered T. litoralis Sh1B DNA polymerase's ability to read through the template-strand uracils by applying SDCM. Specific modification of individual cysteine residues in U-binding pocket - targets introduced into certain positions by site-directed mutagenesis - enables the enzyme to effectively replicate DNA containing uracils. We demonstrate that the acquired resistance of chemically modified T. litoralis Sh1B DNA polymerase to DNA uracil correlates with its decreased affinity for template-strand uracil.
    No preview · Article · Feb 2010 · Biochimica et Biophysica Acta
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