Nanodisk Codes

Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113, USA.
Nano Letters (Impact Factor: 13.59). 01/2008; 7(12):3849-53. DOI: 10.1021/nl072606s
Source: PubMed


We report a new encoding system based upon dispersible arrays of nanodisks prepared by on-wire lithography and functionalized with Raman active chromophores. These nanodisk arrays are encoded both physically (in a "barcode" pattern) and spectroscopically (Raman) along the array. These structures can be used in covert encoding strategies because of their small size or as biological labels with readout by scanning confocal Raman spectroscopy. As proof-of-concept, we demonstrate their utility in DNA detection in a multiplexed format at target concentrations as low as 100 fM.

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    • "Other multiplex SERS methods have also been developed for biosensing based on a microarray approach [114,115], multiple dye-labeling [116,117], or even on “bar-coded” noble metal NPs [118–120]. An example of the latter, are the gold barcode nanodisks fabricated by Mirkin’s group using on-wire lithography to create a barcode with 2 nm separated patterns of nanodisks over nickel wires [119]. The gaps located between the gold nanodisks acted both as “hot-spots”, to enhance the SERS signal from the Raman-active dyes, and as a physical encoded “barcode” pattern, for multiplex detection of biomolecules using confocal Raman spectroscopy. "
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