[RAPD analysis on genetic relationship among varieties of Rehmannia glutinosa].
To define the genetic relationship and other genetic characters of different varieties of Rehmannia glutinosa.
RAPD reactions were conducted on total 54 samples from 16 varieties and 2 populations of R. glutinosa. The data were analyzed by Phylips, Popgene and Arlequin software.
The majority rule consensus tree defined the 54 samples to 3 groups. Population genetics analysis and AMOVA showed that the genetic diversity among varieties/populations much greater than that within varieties/populations, but within group II the genetic diversity among varieties was similar to that within varieties.
Due to the long-time asexual propagation of R. glutinosa, some genetic differentiation has been accumulated and fixed within the species. It was shown that the genetic distance between wild population and cultivated varieties was greater than the genetic distance among cultivated varieties. The wild resource of the plant should be paid more attention and studies.
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- "Therefore, it is imperative to develop efficient molecular markers that identify genetic relationship of different cultivars and distinguish valuable wild germplasms. Although a few molecular markers, including AFLP (Yuan and Hong, 2003), RAPD (Chen et al., 2002; Zhang et al., 2006; Wu et al., 2007; Zhou et al., 2007; Wang et al., 2008), ISSR (Zhou et al., 2007, Wang et al., 2008), SRAP (Zhou et al., 2010) have been reported to identify genetic relationships in various R. glutinosa germplasms, their utilization has been restricted by limited polymorphic information content (Zhang et al., 2012). A recently published set of 15 polymorphic EST-SSR markers that were identified based on Rehmannia species are available (Liu et al., 2015). "
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ABSTRACT: SRAP analytic system was used to assess genetic diversity of Rehmnnia glutinosa. Twenty-three Rehmnnia glutinosa cultivars were screened with 288 primer combinations, of which 13 produced stable and reproducible amplification patterns in three repetitive experiments. Among a total of 338 amplified fragments, 306 (90.5%) were polymorphic, with an average of 23.5 fragments for each primer combination. The percentage of polymorphic bands for each primer combination varied from 58.3 to 100%. The cultivars had a similarity ranging from 0.335 to 0.713 with a mean of 0.518. Shannon's diversity index and expected heterozygosity were 0.3217 and 0.2008, respectively. Based on the cluster, which were conducted on the similarity matrix of SRAP marker data, the cultivars were divided into four groups at the 20 rescaled distance cluster combine. The results demonstrated that SRAP is a stable marker technique for the assessment of genetic diversity of Rehmannia glutinosa cultivars, and that the level of genetic diversity among them from different production areas was relatively high.
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