An essential oil and its major constituent isointermedeol induce apoptosis by increased expression of mitochondrial cytochrome c and apical death receptors in human leukaemia HL-60 cells. Chem Biol Interact

Indian Institute of Integrative Medicine, Jammu, India.
Chemico-Biological Interactions (Impact Factor: 2.58). 03/2008; 171(3):332-47. DOI: 10.1016/j.cbi.2007.10.003
Source: PubMed


An essential oil from a lemon grass variety of Cymbopogon flexuosus (CFO) and its major chemical constituent sesquiterpene isointermedeol (ISO) were investigated for their ability to induce apoptosis in human leukaemia HL-60 cells because dysregulation of apoptosis is the hallmark of cancer cells. CFO and ISO inhibited cell proliferation with 48 h IC50 of approximately 30 and 20 microg/ml, respectively. Both induced concentration dependent strong and early apoptosis as measured by various end-points, e.g. annexinV binding, DNA laddering, apoptotic bodies formation and an increase in hypo diploid sub-G0 DNA content during the early 6h period of study. This could be because of early surge in ROS formation with concurrent loss of mitochondrial membrane potential observed. Both CFO and ISO activated apical death receptors TNFR1, DR4 and caspase-8 activity. Simultaneously, both increased the expression of mitochondrial cytochrome c protein with its concomitant release to cytosol leading to caspase-9 activation, suggesting thereby the involvement of both the intrinsic and extrinsic pathways of apoptosis. Further, Bax translocation, and decrease in nuclear NF-kappaB expression predict multi-target effects of the essential oil and ISO while both appeared to follow similar signaling apoptosis pathways. The easy and abundant availability of the oil combined with its suggested mechanism of cytotoxicity make CFO highly useful in the development of anti-cancer therapeutics.

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    • "Among plant-derived natural products, essential oils, which are concentrated hydrophobic liquids containing volatile aroma compounds from plants, have recently drawn scientific interest for their medicinal applications [19] [20] [21] [22]. Essential oils are endowed with various pharmacological effects, including antibacterial [23], antioxidant [24], antifungal [25], and antimutagenic [26]; and their assorted therapeutic potential has attracted the attention of many researchers to explore their anticancer activities [27] [28] [29] [30]. Schisandrae semen (Schisandra chinensis [Turcz.] "
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    ABSTRACT: The aim of this study was to evaluate the beneficial effects of Schisandrae semen essential oil (SSeo) on apoptosis events and the mechanisms associated with these effects in human leukemia U937 cells. The treatment of U937 cells with SSeo significantly inhibited survival and induced apoptosis. Schisandrae semen essential oil treatment increased the levels of death receptors and Fas, and activated caspases accompanied by proteolytic degradation of poly(ADP-ribose)-polymerase, which was associated with the downregulation of members of the inhibitor of apoptosis protein family protein expression; however, a pan-caspase inhibitor reversed SSeo-induced apoptosis. Treating the cells with SSeo also caused truncation of Bid, translocation of proapoptotic Bax to the mitochondria, and loss of mitochondrial membrane permeabilization, thereby inducing the release of cytochrome c into the cytosol. Subsequently, SSeo upregulated the translocation of mitochondrial apoptogenic factors, such as endonuclease G and apoptosis-inducing factor, into the nucleus during the apoptotic process. Notably, SSeo immediately increased the generation of intracellular reactive oxygen species (ROS); however, pretreatment with N-acetylcysteine, a common ROS quencher, almost completely blocked SSeo-induced apoptosis. Taken together, these findings indicate that SSeo caused ROS- and caspase-dependent cell death involving mitochondrial dysfunction and nuclear translocation of mitochondrial proapoptosis proteins. Based on our data, the consumption of Schisandrae semen or its essential oil is a good natural therapeutic agent for anticancer activity and regression. Copyright © 2015. Published by Elsevier Inc.
    Full-text · Article · Jul 2015 · Nutrition research
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    • "Simultaneously, both increase the expression of mitochondrial cytochrome c protein with its concomitant release to cytosol leading to caspase-9 activation. Further, Bax translocation and decrease in nuclear NFkappaB expression predict multi-target effects of isointermedeol while both appeared to follow similar signaling apoptosis pathways[80]. Furanodiene, a sesquiterpene extracted from Curcuma wenyujin, enhances mitochondrial transmembrane depolarization, release of mitochondrial cytochrome c, activation of cas- pases-3 and cleavage of PARP[81]. Furanodiene mediated mitochondria-caspase apoptotic pathway also involves activation of p38 and inhibition of ERK mitogen-activated protein kinase (MAPK) signaling[81]. "
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    ABSTRACT: Essential oils are widely used in pharmaceutical, sanitary, cosmetic, agriculture and food industries for their bactericidal, virucidal, fungicidal, antiparasitical and insecticidal properties. Their anticancer activity is well documented. Over a hundred essential oils from more than twenty plant families have been tested on more than twenty types of cancers in last past ten years. This review is focused on the activity of essential oils and their components on various types of cancers. For some of them the mechanisms involved in their anticancer activities have been carried out
    Full-text · Article · Nov 2014 · American Journal of Cancer Research
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    • "Of several prescription drugs in use for cancer treatment, almost 75% are derived from plant species (Craig, 1999). It is surprising to note that essential oils, which are found abundantly in nature, have never been exploited for their anticancer potential, although they have found extensive use in perfumery, aromatherapy, food and flavors, etc. since ages (Kumar et al., 2008). Many essential oils or their constituents are known to be the potent antibacterial as well as anti-fungal agents. "
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    ABSTRACT: The essential oils isolated from Tagetes minuta L. flowers and Ocimum basilicum L. herb were analyzed by GC/MS and assessed for antioxidant and in vitro and in vivo anticancer activities. Also biological effects of these essential oils on normal mice were studied. The major components of marigold essential oil were cis-β-ocimene (54.82%), cis-tagetone (11.50%) and trans-tagetenone (10.78%), cistagetenone (7.10%), dihydrotagetone (6.50%) and limonene (3.82%). The major components of basil essential oil were estragole (75.45%), 1,8-cineole (7.56%), linalool (5.01%), trans-anethole (3.72%) and methyleugenol (3.48%). The DPPH · scavenging activities of both essential oils were determined. 50% effective concentration (EC50) of marigold essential oil (86.35 �g/ml) was higher than basil essential oil (80.84 �g/ml). The anticancer activity of the two essential oils on two human promyelocytic leukemia cell lines (HL-60 and NB4) and experimental animals model cancer cell line (EACC) were investigated in vitro. The results indicated that the anticancer activity of marigold essential oil was higher than basil essential oil against NB4 and EACC cell lines, while basil essential oil was higher than marigold essential oil against HL-60 cell line. In in vivo study, pre-initiation treatments with the both essential oils were more effective than initiation and post-initiation treatments, respectively on the tumor (EACC) transplanted female mice. Biological effects of both essential oils on normal mice indicated that all the obtained values in all experimental animals were within the normal range.
    Full-text · Article · Mar 2013
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