Multiple Rare Nonsynonymous Variants in the Adenomatous Polyposis Coli Gene Predispose to Colorectal Adenomas

Institute of Medical Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom.
Cancer Research (Impact Factor: 9.33). 02/2008; 68(2):358-63. DOI: 10.1158/0008-5472.CAN-07-5733
Source: PubMed


It has been proposed that multiple rare variants in numerous genes collectively account for a substantial proportion of multifactorial inherited predisposition to a variety of diseases, including colorectal adenomas (CRA). We have studied this hypothesis by sequencing the adenomatous polyposis coli (APC) gene in 691 unrelated North American patients with CRAs and 969 matched healthy controls. Rare inherited nonsynonymous variants of APC were significantly overrepresented in patients who did not carry conventional pathogenic mutations in the APC or MutY homologue genes [non-familial adenomatous polyposis (FAP) non-MUTYH-associated polyposis (MAP) patients; 81 of 480, 16.9%] compared with patients with FAP or MAP (20 of 211, 9.5%, P = 0.0113), and this overrepresentation was highest in those non-FAP non-MAP patients with 11 to 99 CRAs (30 of 161, 18.6%, P = 0.0103). Furthermore, significantly more non-FAP non-MAP patients carried rare nonsynonymous variants in the functionally important beta-catenin down-regulating domain compared with healthy controls (32 of 480 versus 37 of 969, P = 0.0166). In silico analyses predicted that approximately 46% of the 61 different variants identified were likely to affect function, and upon testing, 7 of 16 nonsynonymous variants were shown to alter beta-catenin-regulated transcription in vitro. These data suggest that multiple rare nonsynonymous variants in APC play a significant role in predisposing to CRAs.

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    • "This is particularly important for addressing the effects of multiple rare variants that cause functional damage in a combinatorial manner. Previous studies identified rare predisposing variants that are significantly associated with complex traits such as colorectal adenomas,95 high-density lipoprotein cholesterol,96 and schizophrenia.97 There are two major types of variant association tests: (1) burden tests, which include collapsing methods such as CAST,98 CMC,99 RareCover,100 aSum,101 and aggregation methods such as WSS,102 KBAC, and103 RBT104) (2) non-burden test based methods such as VT,105 C-alpha,106 EREC,107 and SKAT.108 "
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    • "We previously predicted those 2000 amino acids as largely intrinsically disordered [18]. Interestingly, this segment contains the most frequently mutated stretch of APC, the mutation cluster region (MCR), which contains three β-catenin binding 20aa repeats [27,28]. It is unclear how the structural properties of APC-MCR relate to β-catenin recognition and regulation by phosphorylation. "
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