Anti- inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia mill. J Ethnopharmacol

Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Journal of Ethnopharmacology (Impact Factor: 3). 12/2003; 89(1):67-71. DOI: 10.1016/S0378-8741(03)00234-4
Source: PubMed

ABSTRACT

Extracts obtained from the leaves of Lavandula angustifolia Mill. (Lamiaceae) are used in Iranian folk medicine as remedies for the treatment of various inflammatory diseases. For evaluation of its probable analgesic and anti-inflammatory effects, hydroalcoholic extract, polyphenolic fraction and essential oil of the leaves of the herb were prepared and their analgesic effects were studied in mice using formalin and acetic acid-induced writhing tests. Carrageenan test in rats was used for assessment of anti-inflammatory activity of above-mentioned fractions. Results showed that while the hydroalcoholic extract (400-1600 mg/kg, p.o.) inhibited only the second phase of formalin test, the polyphenolic fraction (800 and 1600 mg/kg, p.o.) and essential oil (100 and 200 mg/kg, p.o.) suppressed both phases. In acetic acid-induced writhing test, polyphenolic fraction (400 and 800 mg/kg, p.o.) and essential oil (100 and 200 mg/kg, p.o.) reduced the number of abdominal constrictions. Essential oil at a dose of 200mg/kg also inhibited carrageenan-induced paw edema. Results of the present study confirm the traditional use of Lavandula angustifolia for the treatment of painful and inflammatory conditions and calls for further investigations to determine the active chemical constituent(s).

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Available from: Alireza Ghannadi, Feb 09, 2014
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    • "test, but only the phenolic and alcoholic extracts had been able to prevent the first phase of the formalin test. Also application of this plant could not prevent the edema evoked by carrageenin administration (Hajhashemi and Ghannadi, 2003). It was proved that inhaling the leaves of the Lavandula officinalis could attenuate pain evoked by hotplate test, and stomach graze induced by high dose administration of ethanol and ascetic acid (Barocelli et al., 2004). "
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    ABSTRACT: Lavandula officinalis Chaix, Lamiaceae, extracts can inhibit inflammation and also pain induced by formalin in mice. This study evaluated the effects of L. officinalis hydro-alcoholic extract on pain induced by formalin and also cyclooxygenase (COX) type 1 and 2 activity in mice. To evaluate probable analgesic and anti-inflammatory effects of the extract, flowers were prepared by maceration and extraction in alcohol and their analgesic effects were studied in male mice, using formalin and hot plate tests. The effect of intraperitoneal hydro-alcoholic extracts of L. officinalis (100, 200, 250, 300, 400 and 800mg/kg), subcutaneous morphine (10mg/kg), dexamethasone (10mg/kg; i.p.) and indomethacin (10mg/kg; i.p.) on formalin induced pain were studied. Our results indicated that administration of the extract (100, 200, 250, 300, 400 and 800mg/kg; i.p.) has inhibitory effects on inflammation induced by formalin injection into the animals hind paw. Moreover, this inhibitory effect was equal to the effects of morphine, dexamethasone and indomethacin. The extract in100, 200 and 300mg/kg; significantly reduced heat-induced pain. The extract also reduced COX activity in dose dependent manner, where the inhibitory effect on COX1 activity was 33% and on COX2 activity was 45%. Here for the first time we show that L. officinialis extract can modulate pain and inflammation induced by formalin by inhibition of COX enzymes.
    Full-text · Article · Nov 2015 · Revista Brasileira de Farmacognosia
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    • "The ethanolic extract was prepared by mechanic maceration of the plant's powder, with ethanol in PA (IMPEX, Brazil) (at a concentration of 1:10 [p/v]) for 24 h at room temperature. The extract was then filtered at low pressure under vacuum, a methodology similar to the one performed by Rutherford and Powrie (1993), Hajhashemi et al. (2003) and Boligon et al. (2009). "

    Preview · Article · Mar 2015 · African journal of agricultural research
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    • "In India, it has been successfully introduced for its cultivation in temperate regions of Kashmir Valley (Tajjudin et al. 1983; Shawl and Kumar 2000). In addition to its perfumery value, lavender oil is also used in aromatherapy to relieve depression, insomnia, migraine headache, nerve sprains and joint pains (Cavanagh and Wilkinson 2002; Hajhashemi et al. 2003; Sasannejad et al. 2012). Lavandula species are conventionally propagated through seeds or stem cuttings. "
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    ABSTRACT: A protocol for long-term in vitro conservation of multiple shoot cultures of Lavandula officinalis and regeneration of true-to-type plants from them is described here. Multiple shoots were developed from apical bud explants on a modified vitamin-enriched Murashige and Skoog’s basal medium supplemented with 2.5 mg/l Kinetin. The cultures were subsequently conserved in vitro for 6 years under slow growth conditions imposed by lowering the sucrose level from 3 to 2.0 % and increasing the agar concentration from 0.8 to 1.2 % in this medium. Complete plant regeneration from in vitro conserved shoots was achieved through axillary shoot proliferation on original 2.5 mg/l Kinetin containing medium followed by rooting of individual shoots in a hormone-free half strength MS basal medium. The genetic fidelity of the regenerated plants was tested by random amplified polymorphic DNA analysis. Twenty one arbitrary decamer primers produced a total of 64 scorable bands (1–6 bands/primer) in the size range of 100–5,148 bp. All DNA fingerprints with these primers displayed monomorphic band profiles indicating homogeneity among the regenerated progeny and their uniformity with the donor parent. Head-space gas chromatographic analysis of the leaf tissue excised from mother as well as regenerated plants also revealed a similar qualitative and quantitative profile of volatile terpenes. The utility of the developed protocol for long-term ex situ conservation and quality plant production for cultivation of this high value aromatic herb is discussed.
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