The calcium-binding protein S100P in normal and malignant human tissues

Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
BMC Clinical Pathology 02/2008; 8(1):2. DOI: 10.1186/1472-6890-8-2
Source: PubMed


S100P is a Ca2+ binding protein overexpressed in a variety of cancers, and thus, has been considered a potential tumor biomarker. Very little has been studied about its normal expression and functions.
We examined S100P expression in normal human tissues by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. S100P protein expression was also studied in a series of tumors, consisting of 74 ovarian, 11 pancreatic, 56 gastric, 57 colorectal, 89 breast and 193 prostate carcinomas using a novel anti-S100P monoclonal antibody.
Among the normal tissues, the highest S100P mRNA levels were observed in the placenta and esophagus. Moderate signals were also detected in the stomach, duodenum, large intestine, prostate and leukocytes. At the protein level, the highest reactions for S100P were seen in the placenta and stomach. Immunostaining of tumor specimens showed that S100P protein is expressed in all the tumor categories included in the study, being most prevalent in gastric tumors.
Based on our observations, S100P is widely expressed in both normal and malignant tissues. The high expression in some tumors suggests that it may represent a potential target molecule for future diagnostic and therapeutic applications.

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    • "Nuclear, cytoplasmic, or membrane localization has been recognized in different experimental settings [7] [10]. Parkkila et al. [7] showed strong nuclear staining in breast carcinoma sections, with some apparently cytoplasmic staining also present similar to our observation in Fig. 3E. Maciejczyk et al. [6] reported nuclear staining in 73% of breast cancers and cytoplasmic staining in 69% (the majority classified as borderline or weak). "
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    • "c o m / l o c a t e / y e x m p angiogenesis, cytoskeleton interactions, protein phosphorylation, regulation of transcriptional factors, autoimmunity, chemotaxis, inflammation and pluripotency and metastasis (Chen et al., 2014; Arumugam et al., 2004; Wang et al., 2006). Overexpression of S100P has been reported in many cancers (Parkkila et al., 2008; Jiang et al., 2012). Several studies have investigated S100P in the carcinogenesis and development of breast cancer (Guerreiro Da Silva et al., 2000; Schor et al., 2006). "
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    • "A few of these genes seemed to be particularly interesting. For example, S100P calcium binding protein (which expression is regulated by androgens and IL6 – another up-regulated gene in the most malignant canine mammary tumours) is though as a new prognostic factor (Parkkila et al. 2008). A correlation was found between its increased expression and poor survival, cancer proliferation and increased resistance to chemotherapy (Maciejczyk et al. 2013). "
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