Article

Microbacterium hatanonis sp nov., isolated as a contaminant of hairspray

Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 04/2008; 58(Pt 3):654-8. DOI: 10.1099/ijs.0.65160-0
Source: PubMed

ABSTRACT

An aerobic, rod-shaped, Gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain FCC-01(T), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. A 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Microbacterium and represented an evolutionary lineage that was distinct from recognized Microbacterium species. Cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars consisted of rhamnose and galactose. The main respiratory quinones were MK-12 (38 %) and MK-11 (35 %). The major cellular fatty acids were anteiso-C(15 : 0) (48 %), anteiso-C(17 : 0) (35 %) and iso-C(16 : 0) (11 %). The DNA G+C content was 69 mol%. The isolate showed <98 % 16S rRNA gene sequence similarity with respect to all of the Microbacterium species with validly published names. On the basis of the morphological, physiological and chemotaxonomic data and the results of the comparative 16S rRNA gene sequence analysis, this isolate represents a novel species of the genus Microbacterium, for which the name Microbacterium hatanonis sp. nov. is proposed. The type strain is FCC-01(T) (=JCM 14558(T) =DSM 19179(T)).

  • Source
    • "At the time of writing, the genus Microbacterium comprised 72 species of Gram-staining-positive bacteria (http://www. bacterio.cict.fr/m/microbacterium.html), which were isolated from diverse habitats, including soil, sea sediments, insects, human clinical specimens and plants (Evtushenko & Takeuchi, 2006; Park et al., 2008; Bakir et al., 2008; Takeuchi & Hatano, 1998a, b; Lee et al., 2006; Shivaji et al., 2007; Collins & Bradbury, 1992). Current members of the genus Microbacterium have N-glycolyl residues in their cell walls, menaquinones with 10–14 (mainly 11–13) completely unsaturated isoprenoid units as their respiratory quinones, and genomic DNA G+C contents of 64– 75 mol% (Lal et al., 2010). "
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-staining-positive, heterotrophic, aerobic, non-motile, non-endospore-forming, yellow-coloured rod, designated strain N5(T), was isolated from a soil sample collected at an industrial waste site in Noida, on the outskirts of Delhi, India. In phylogenetic analyses based on 16S rRNA gene sequences, strain N5(T) was most closely related to members of established species in the genus Microbacterium (with sequence similarities of approximately 94.0-97.6 %), particularly Microbacterium indicum LMG 23459(T) (97.59 %) and Microbacterium gubbeenense LMG 19263(T) (97.18 %). In DNA-DNA hybridization studies, however, none of the DNA-DNA relatedness values between strain N5(T) and members of the genus Microbacterium exceeded 11.3 %. The genomic DNA G+C content of the novel strain was 68 mol%. The chemotaxonomic characteristics of strain N5(T), which had MK-11 and MK-10 as its major menaquinones and anteiso-C(15 : 0) (45 %), anteiso-C(17 : 0) (37 %), iso-C(16 : 0) (8.5 %) and C(16 : 0) (4.5 %) as its predominant fatty acids, were consistent with classification in the genus Microbacterium. Peptidoglycan in the novel strain, which contained ornithine, alanine, glycine, homoserine, glutamic acid, 3-hydroxyglutamic acid, muramic acid and traces of N-glycolyl residues, was of type B2β. The polar lipid profile of strain N5(T) comprised diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The novel strain's major cell-wall sugars were glucose and galactose. Based on the phylogenetic, DNA-DNA hybridization, chemotaxonomic and phenotypic data, strain N5(T) represents a novel species within the genus Microbacterium for which the name Microbacterium amylolyticum sp. nov. is proposed; the type strain is N5(T) ( = DSM 24221(T) = CCM 7881(T)).
    Preview · Article · Oct 2011 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    • "Members of the genus Microbacterium can be isolated from a wide range of different environmental habitats from soil to insects to human clinical specimens to marine environments (Evtushenko & Takeuchi, 2006; Park et al., 2008; Bakir et al., 2008; Takeuchi & Hatano, 1998a, b; Lee et al., 2006; Shivaji et al., 2007; Collins & Bradbury, 1992) and plants, having been reported from the phyllosphere of sugar beet and spring wheat, and as endophytes in sweet corn and cotton (Thompson et al., 1993; Legard et al., 1994; McInroy & Kloepper, 1995). The genus Microbacterium , established by Orla-Jensen (1919) and emended by Collins et al. (1983), comprises a diverse collection of Gram-positive, non-spore-forming, rod-shaped bacteria. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Microbacterium strain AI-S262(T) was isolated from the rhizoplane of neem seedlings in the Botanical garden of Tamilnadu Agricultural University, Coimbatore, India, and subjected to phenotypic, chemotaxonomic and genetic characterization. Cells of this strain were Gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. Strain AI-S262(T) contained MK-12 and MK-13 as the main respiratory quinones, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as the predominant fatty acids, peptidoglycan-type B2beta with glycolyl residues, and had a DNA G+C content of 69.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed 98.0-98.6 % pair-wise similarity with respect to close relatives in the genus Microbacterium. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 39%) between strain AI-S262(T) and its closest relatives. Data from DNA-DNA hybridization and phenotypic analyses supported the conclusion that strain AI-S262(T) represents a novel species in the genus Microbacterium, for which the name Microbacterium azadirachtae sp. nov. is proposed. The type strain is AI-S262(T) (=JCM 15681(T) =LMG 24772(T) =KCTC 19668(T)).
    Full-text · Article · Oct 2009 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    • "The genus Microbacterium was proposed by Orla-Jensen (1919); at the time of writing, the genus comprised 50 species with validly published names, including several described since 2006: Microbacterium koreense (Lee et al., 2006), M. paludicola (Park et al., 2006), M. aoyamense, M. deminutum and M. pumilum (Kageyama et al., 2006), M. indicum (Shivaji et al., 2007), M. sediminicola and M. marinilacus (Kageyama et al., 2007), M. ginsengisoli (Park et al., 2008) and M. hatanonis (Bakir et al., 2008). Here we report on the taxonomic characterization of a Microbacterium-like strain, designated DS-66 T , which was isolated from soil of Dokdo, Korea. "
    [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-positive, non-motile, rod- or coccoid-shaped Microbacterium-like bacterium, designated strain DS-66(T), was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66(T) grew optimally at 30 degrees C and pH 6.5-7.0 in the presence of 0.5-1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-66(T) belonged to the genus Microbacterium. Strain DS-66(T) had a peptidoglycan type based on B2beta with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg-Gly-d-Orn), and galactose, rhamnose and ribose as whole-cell sugars. The acyl type was glycolyl. Strain DS-66(T) contained MK-13, MK-12 and MK-14 as predominant menaquinones and anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 69.9 mol%. Phylogenetic distinctiveness, DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain DS-66(T) is distinguishable from recognized Microbacterium species. On the basis of the data presented, strain DS-66(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium insulae sp. nov. is proposed. The type strain is DS-66(T) (=KCTC 19247(T)=CCUG 54523(T)).
    Preview · Article · Jul 2009 · International Journal of Systematic and Evolutionary Microbiology
Show more